Construct: sgRNA BRDN0001162239
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTTGCACACTGATCAATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRIM33 (51592)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77098
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
114424747 |
+ |
TRIM33 |
NNNTGCACACTGATCAATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
119633328 |
+ |
ZNF697 |
NNNTGCACACTGATTAATCA |
NGG |
2 |
0.0625 |
Tier II |
| 3 |
NC_000021.9 |
21 |
38772338 |
- |
LINC00114 |
NNNTGCACACTGAACAATCA |
NGG |
2 |
0.5804 |
Tier III |
| 4 |
NC_000004.12 |
4 |
186308195 |
- |
F11-AS1 |
NNNTGCACACTGACCTATCG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
103330089 |
- |
Trim33 |
NNNTGCACACTGATCAATCT |
NGG |
1 |
0.7 |
Tier I |
| 2 |
NC_000070.6 |
4 |
12070478 |
+ |
Tmem67 |
NNNTGCACACTGATCAATCC |
NGC |
2 |
0.0095 |
Tier I |
| 3 |
NC_000075.6 |
9 |
16140296 |
+ |
Fat3 |
NNNTGCACATTGATCAATAG |
NGG |
2 |
0.4034 |
Tier II |
| 4 |
NC_000070.6 |
4 |
76560986 |
+ |
Ptprd |
NNNTGCACACTGACCAATTG |
NGG |
2 |
0.1319 |
Tier II |
| 5 |
NC_000076.6 |
10 |
58274273 |
- |
Gcc2 |
NNNTTCACACTGATCATTCG |
NGG |
2 |
0.04 |
Tier II |
| 6 |
NC_000068.7 |
2 |
132146045 |
+ |
Gm14051 |
NNNAGCACACTGATCAATAG |
NGG |
2 |
0.2041 |
Tier III |
| 7 |
NC_000077.6 |
11 |
26113099 |
- |
5730522E02Rik |
NNNTGCACACTGATCCATCT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
