Construct: sgRNA BRDN0001162254
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTGCGGTCTGCTTGTAGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80209
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
61982857 |
+ |
MED13 |
NNNGGGGTCTGCATGTAGAT |
NGG |
2 |
0.4154 |
Tier I |
| 2 |
NC_000012.12 |
12 |
110869538 |
+ |
CCDC63 |
NNNGCCGTCTGCTTCTAGAT |
NGG |
2 |
0.186 |
Tier II |
| 3 |
NC_000006.12 |
6 |
33732863 |
- |
IP6K3 |
NNNGAGGTCTGCTCGTAGAT |
NGG |
2 |
0.1633 |
Tier II |
| 4 |
NC_000004.12 |
4 |
108060685 |
- |
LEF1 |
NNNGGGGTCTGCTTTTAGAT |
NGG |
2 |
0.0857 |
Tier II |
| 5 |
NC_000007.14 |
7 |
132432596 |
+ |
PLXNA4 |
NNNGAGGTCTGCTTGTTGAT |
NGG |
2 |
0.0762 |
Tier II |
| 6 |
NC_000003.12 |
3 |
153490847 |
+ |
LINC02877 |
NNNGCAGTCTGCCTGTAGAT |
NGG |
2 |
0.7895 |
Tier III |
| 7 |
NC_000003.12 |
3 |
153490847 |
+ |
LINC02006 |
NNNGCAGTCTGCCTGTAGAT |
NGG |
2 |
0.7895 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
83767851 |
- |
Ankrd53 |
NNNGTGGTCTGCTTGTAGAT |
NGG |
1 |
0.6364 |
Tier I |
| 2 |
NC_000069.6 |
3 |
135618840 |
+ |
Nfkb1 |
NNNGCCTTCTGCTTGTAGAT |
NGG |
2 |
0.3896 |
Tier I |
| 3 |
NC_000074.6 |
8 |
84087743 |
- |
Rfx1 |
NNNGCCGTCTGCGTGTAGAT |
NGG |
2 |
0.1779 |
Tier I |
| 4 |
NC_000068.7 |
2 |
168679497 |
- |
Atp9a |
NNNGCGCTCTGCTTGTTGAT |
NGG |
2 |
0.0917 |
Tier II |
| 5 |
NC_000069.6 |
3 |
79219857 |
+ |
Rapgef2 |
NNNGCGGTCTGCTTGTGGGT |
NGG |
2 |
0.0662 |
Tier II |
| 6 |
NC_000067.6 |
1 |
194262423 |
+ |
Gm31988 |
NNNTCTGTCTGCTTGTAGAT |
NGG |
2 |
0.2424 |
Tier III |
| 7 |
NC_000069.6 |
3 |
79219857 |
+ |
4921511C10Rik |
NNNGCGGTCTGCTTGTGGGT |
NGG |
2 |
0.0662 |
Tier III |
Other clones with same target sequence:
(none)
