Construct: sgRNA BRDN0001162256
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCCCGGGAGTATTACAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMK2A (815)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76929
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
150256782 |
- |
CAMK2A |
NNNCCGGGAGTATTACAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
140451046 |
+ |
TRAPPC9 |
NNNCCGGGAGTCATACAGTG |
NGG |
2 |
0.1822 |
Tier I |
| 3 |
NC_000002.12 |
2 |
133543851 |
+ |
NCKAP5 |
NNNCTTGGAGTATTACAGTG |
NGG |
2 |
0.4242 |
Tier II |
| 4 |
NC_000019.10 |
19 |
4175376 |
+ |
SIRT6 |
NNNCGGGGAGTATCACAGTG |
NGG |
2 |
0.1714 |
Tier II |
| 5 |
NC_000007.14 |
7 |
33403256 |
- |
BBS9 |
NNNCTGGGAGTATTAGAGTG |
NGG |
2 |
0.0979 |
Tier II |
| 6 |
NC_000020.11 |
20 |
17381295 |
+ |
PCSK2 |
NNNCCGGGAGTATTATGGTG |
NGG |
2 |
0.0543 |
Tier II |
| 7 |
NC_000001.11 |
1 |
70729182 |
- |
LINC01788 |
NNNCCTGGGGTATTACAGTG |
NGG |
2 |
0.4444 |
Tier III |
| 8 |
NC_000006.12 |
6 |
70363233 |
- |
LOC105377848 |
NNNCCTGGAGTATTACAGTG |
NGT |
2 |
0.0108 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
60952184 |
+ |
Camk2a |
NNNCCGGGAGTATTACAGTG |
NAG |
1 |
0.2593 |
Tier I |
| 2 |
NC_000075.6 |
9 |
49353368 |
- |
Drd2 |
NNNCCTGGAGTTTTACAGTG |
NGG |
2 |
0.2222 |
Tier II |
Other clones with same target sequence:
(none)
