Construct: sgRNA BRDN0001162263
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGACGTTGCCTGCTTTGCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKQ (1609)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77379
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
965262 |
- |
DGKQ |
NNNCGTTGCCTGCTTTGCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
236894469 |
+ |
MTR |
NNNCGTTGCCTGCTTTGGGG |
NAG |
2 |
0.0346 |
Tier I |
| 3 |
NC_000017.11 |
17 |
41782477 |
- |
JUP |
NNNAGTTGCCTGCTTTGCTG |
NGG |
2 |
0.5614 |
Tier II |
| 4 |
NC_000011.10 |
11 |
11258253 |
- |
LOC124902631 |
NNNGGTTGCCTGCTCTGCGG |
NGG |
2 |
0.1364 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
12987889 |
+ |
Mcf2l |
NNNCGTTCCCTGCTTTGCGG |
NGG |
1 |
0.6154 |
Tier II |
| 2 |
NC_000073.6 |
7 |
127001438 |
- |
Mvp |
NNNCGCTGCCTGCTCTGCGG |
NGG |
2 |
0.2479 |
Tier II |
| 3 |
NC_000084.6 |
18 |
63708860 |
+ |
Wdr7 |
NNNCTTTGCCTTCTTTGCGG |
NGG |
2 |
0.1154 |
Tier II |
| 4 |
NC_000070.6 |
4 |
8790188 |
+ |
Chd7 |
NNNCGTTCCCTGCTTTGGGG |
NGG |
2 |
0.0821 |
Tier II |
| 5 |
NC_000071.6 |
5 |
107289188 |
- |
Tgfbr3 |
NNNCGGTGCCTGCTTTGCGG |
NGT |
2 |
0.0092 |
Tier II |
| 6 |
NC_000077.6 |
11 |
86382828 |
- |
Gm38937 |
NNNCGTTGCCTGCTTTCCGG |
NGC |
2 |
0.0052 |
Tier III |
Other clones with same target sequence:
(none)
