Construct: sgRNA BRDN0001162265
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCGGTATGGACTCTCCACGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PLXNA4 (91584)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76879
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
132223628 |
+ |
PLXNA4 |
NNNGTATGGACTCTCCACGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
141956447 |
- |
PCDH12 |
NNNGTATGAAGTCTCCACGC |
NGG |
2 |
0.1607 |
Tier I |
| 3 |
NC_000012.12 |
12 |
121295624 |
- |
CAMKK2 |
NNNGTTTGGAGTCTCCACGC |
NGG |
2 |
0.1786 |
Tier II |
| 4 |
NC_000005.10 |
5 |
141956447 |
- |
RNF14 |
NNNGTATGAAGTCTCCACGC |
NGG |
2 |
0.1607 |
Tier II |
| 5 |
NC_000009.12 |
9 |
98315991 |
+ |
GABBR2 |
NNNGTGTGGACTGTCCACGC |
NGG |
2 |
0.0974 |
Tier II |
| 6 |
NC_000016.10 |
16 |
19440021 |
- |
TMC5 |
NNNGTATGGACTCTTCACCC |
NGG |
2 |
0.0299 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
75030377 |
- |
Ltbp1 |
NNNGTATGGAATCACCACGC |
NGG |
2 |
0.4643 |
Tier II |
| 2 |
NC_000083.6 |
17 |
71790358 |
+ |
Clip4 |
NNNGTATGGAATCTCCACGG |
NGG |
2 |
0.0441 |
Tier II |
| 3 |
NC_000067.6 |
1 |
127455695 |
- |
Mgat5 |
NNNGTATGGATTCTCCAGGC |
NGG |
2 |
0.041 |
Tier II |
| 4 |
NC_000067.6 |
1 |
127455695 |
- |
Gm41947 |
NNNGTATGGATTCTCCAGGC |
NGG |
2 |
0.041 |
Tier III |
Other clones with same target sequence:
(none)
