Construct: sgRNA BRDN0001162273

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATCGTGCACAGGAATCTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMKV (79012)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75529

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 49861444 - CAMKV NNNCGTGCACAGGAATCTCA NGG 0 1.0 Tier I
2 NC_000006.12 6 16748172 + ATXN1 NNNAGTGCATAGGAATCTCA NGG 2 0.7926 Tier II
3 NC_000008.11 8 19083107 - PSD3 NNNCATGGACAGGAATCTCA NGG 2 0.5571 Tier II
4 NC_000012.12 12 26943989 + FGFR1OP2 NNNTGTGCCCAGGAATCTCA NGG 2 0.4571 Tier II
5 NC_000010.11 10 19714890 - MALRD1 NNNTGTGCACTGGAATCTCA NGG 2 0.2462 Tier II
6 NC_000009.12 9 109752343 + PALM2AKAP2 NNNCATGCACAGGAATCTCC NGG 2 0.197 Tier II
7 NC_000007.14 7 139712067 - HIPK2 NNNTGTGCACAGGAATCTCC NGG 2 0.1818 Tier II
8 NC_000022.11 22 25873956 + MYO18B NNNCGTGCACAGGAATCCCC NGG 2 0.0974 Tier II
9 NC_000004.12 4 184433235 + IRF2 NNNCGTGCACAGGAATCCCA NGT 2 0.0069 Tier II
10 NC_000004.12 4 98784652 - LOC105377343 NNNCGTGTACAGGAAACTCA NGG 2 0.7955 Tier III
11 NC_000002.12 2 129284896 - LOC105373612 NNNCGTGCAAAGGAATCTCG NGG 2 0.6627 Tier III
12 NC_000005.10 5 177931013 - LOC124901146 NNNCGTGCACAGAAATCTCT NGG 2 0.5538 Tier III
13 NC_000010.11 10 19714890 - MALRD1-AS1 NNNTGTGCACTGGAATCTCA NGG 2 0.2462 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 21309368 - Hbs1l NNNCGTGCACAAGAATCTCT NGG 2 0.56 Tier I
2 NC_000075.6 9 107946213 + Camkv NNNTGTGCACAGGAACCTCA NGG 2 0.5333 Tier I
3 NC_000076.6 10 44132626 - Crybg1 NNNAGTGCACAGGAAACTCA NGG 2 0.7656 Tier II
4 NC_000077.6 11 61159859 - Usp22 NNNAGGGCACAGGAATCTCA NGG 2 0.4812 Tier II
5 NC_000072.6 6 147558565 + Ccdc91 NNNAGTGCACAGGAATCTTA NGG 2 0.3887 Tier II
6 NC_000074.6 8 35466194 + Eri1 NNNCGTCCACAGGAATCTAA NGG 2 0.2946 Tier II
7 NC_000081.6 15 101738545 - Krt71 NNNCTTGCACAGGAAGCTCA NGG 2 0.1038 Tier II
8 NC_000070.6 4 97866735 + Nfia NNNCTTGCACAGGATTCTCA NGG 2 0.06 Tier II
9 NC_000068.7 2 181132660 + Kcnq2 NNNCGTGCTCAGGAATCTCA NGC 2 0.0133 Tier II
10 NC_000074.6 8 81903192 + Inpp4b NNNCGTGCACAGGAATCTTA NGT 2 0.0074 Tier II
11 NC_000076.6 10 72310008 - Gm9923 NNNCGTGCACAAGAATCTCT NGG 2 0.56 Tier III
12 NC_000083.6 17 35184100 - Ncr3-ps NNNCCTGCACACGAATCTCA NGG 2 0.416 Tier III
13 NC_000070.6 4 97866735 + Gm19417 NNNCTTGCACAGGATTCTCA NGG 2 0.06 Tier III
Download CSV

Other clones with same target sequence:

(none)

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