Construct: sgRNA BRDN0001162273
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATCGTGCACAGGAATCTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMKV (79012)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75529
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
49861444 |
- |
CAMKV |
NNNCGTGCACAGGAATCTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
16748172 |
+ |
ATXN1 |
NNNAGTGCATAGGAATCTCA |
NGG |
2 |
0.7926 |
Tier II |
| 3 |
NC_000008.11 |
8 |
19083107 |
- |
PSD3 |
NNNCATGGACAGGAATCTCA |
NGG |
2 |
0.5571 |
Tier II |
| 4 |
NC_000012.12 |
12 |
26943989 |
+ |
FGFR1OP2 |
NNNTGTGCCCAGGAATCTCA |
NGG |
2 |
0.4571 |
Tier II |
| 5 |
NC_000010.11 |
10 |
19714890 |
- |
MALRD1 |
NNNTGTGCACTGGAATCTCA |
NGG |
2 |
0.2462 |
Tier II |
| 6 |
NC_000009.12 |
9 |
109752343 |
+ |
PALM2AKAP2 |
NNNCATGCACAGGAATCTCC |
NGG |
2 |
0.197 |
Tier II |
| 7 |
NC_000007.14 |
7 |
139712067 |
- |
HIPK2 |
NNNTGTGCACAGGAATCTCC |
NGG |
2 |
0.1818 |
Tier II |
| 8 |
NC_000022.11 |
22 |
25873956 |
+ |
MYO18B |
NNNCGTGCACAGGAATCCCC |
NGG |
2 |
0.0974 |
Tier II |
| 9 |
NC_000004.12 |
4 |
184433235 |
+ |
IRF2 |
NNNCGTGCACAGGAATCCCA |
NGT |
2 |
0.0069 |
Tier II |
| 10 |
NC_000004.12 |
4 |
98784652 |
- |
LOC105377343 |
NNNCGTGTACAGGAAACTCA |
NGG |
2 |
0.7955 |
Tier III |
| 11 |
NC_000002.12 |
2 |
129284896 |
- |
LOC105373612 |
NNNCGTGCAAAGGAATCTCG |
NGG |
2 |
0.6627 |
Tier III |
| 12 |
NC_000005.10 |
5 |
177931013 |
- |
LOC124901146 |
NNNCGTGCACAGAAATCTCT |
NGG |
2 |
0.5538 |
Tier III |
| 13 |
NC_000010.11 |
10 |
19714890 |
- |
LOC101928834 |
NNNTGTGCACTGGAATCTCA |
NGG |
2 |
0.2462 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
21309368 |
- |
Hbs1l |
NNNCGTGCACAAGAATCTCT |
NGG |
2 |
0.56 |
Tier I |
| 2 |
NC_000075.6 |
9 |
107946213 |
+ |
Camkv |
NNNTGTGCACAGGAACCTCA |
NGG |
2 |
0.5333 |
Tier I |
| 3 |
NC_000076.6 |
10 |
44132626 |
- |
Crybg1 |
NNNAGTGCACAGGAAACTCA |
NGG |
2 |
0.7656 |
Tier II |
| 4 |
NC_000077.6 |
11 |
61159859 |
- |
Usp22 |
NNNAGGGCACAGGAATCTCA |
NGG |
2 |
0.4812 |
Tier II |
| 5 |
NC_000072.6 |
6 |
147558565 |
+ |
Ccdc91 |
NNNAGTGCACAGGAATCTTA |
NGG |
2 |
0.3887 |
Tier II |
| 6 |
NC_000074.6 |
8 |
35466194 |
+ |
Eri1 |
NNNCGTCCACAGGAATCTAA |
NGG |
2 |
0.2946 |
Tier II |
| 7 |
NC_000081.6 |
15 |
101738545 |
- |
Krt71 |
NNNCTTGCACAGGAAGCTCA |
NGG |
2 |
0.1038 |
Tier II |
| 8 |
NC_000070.6 |
4 |
97866735 |
+ |
Nfia |
NNNCTTGCACAGGATTCTCA |
NGG |
2 |
0.06 |
Tier II |
| 9 |
NC_000068.7 |
2 |
181132660 |
+ |
Kcnq2 |
NNNCGTGCTCAGGAATCTCA |
NGC |
2 |
0.0133 |
Tier II |
| 10 |
NC_000074.6 |
8 |
81903192 |
+ |
Inpp4b |
NNNCGTGCACAGGAATCTTA |
NGT |
2 |
0.0074 |
Tier II |
| 11 |
NC_000076.6 |
10 |
72310008 |
- |
Gm9923 |
NNNCGTGCACAAGAATCTCT |
NGG |
2 |
0.56 |
Tier III |
| 12 |
NC_000083.6 |
17 |
35184100 |
- |
Ncr3-ps |
NNNCCTGCACACGAATCTCA |
NGG |
2 |
0.416 |
Tier III |
| 13 |
NC_000070.6 |
4 |
97866735 |
+ |
Gm19417 |
NNNCTTGCACAGGATTCTCA |
NGG |
2 |
0.06 |
Tier III |
Other clones with same target sequence:
(none)
