Construct: sgRNA BRDN0001162276
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGCCACTTGAGTAAGGCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TBRG4 (9238)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77703
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
45104146 |
+ |
TBRG4 |
NNNCCACTTGAGTAAGGCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
1941825 |
- |
MSRB1 |
NNNCCACTTGAGAAAGGAGA |
NGG |
2 |
0.3728 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
6619603 |
+ |
Tbrg4 |
NNNCCACTTGAGGAAGGCAA |
NGG |
2 |
0.1863 |
Tier I |
| 2 |
NC_000071.6 |
5 |
138276516 |
- |
Stag3 |
NNNCCATTTGAGTAAGGAGA |
NGG |
2 |
0.4375 |
Tier II |
| 3 |
NC_000071.6 |
5 |
138276516 |
- |
Gpc2 |
NNNCCATTTGAGTAAGGAGA |
NGG |
2 |
0.4375 |
Tier II |
| 4 |
NC_000076.6 |
10 |
12653853 |
- |
Utrn |
NNNCCACTTAAGTAAGGCGA |
NAG |
2 |
0.242 |
Tier II |
| 5 |
NC_000081.6 |
15 |
98856497 |
+ |
Kmt2d |
NNNCCACTTGAGTAAGACGA |
NGT |
2 |
0.0151 |
Tier II |
| 6 |
NC_000082.6 |
16 |
25066312 |
- |
A230028O05Rik |
NNNCCACTTGAGTAATGAGA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
