Construct: sgRNA BRDN0001162277
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGACCACTTCTTACCACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERN2 (10595)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75848
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
23702626 |
+ |
ERN2 |
NNNACCACTTCTTACCACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
123159971 |
- |
CLMP |
NNNACAAATTCTTACCACCA |
NGG |
2 |
0.6036 |
Tier II |
| 3 |
NC_000014.9 |
14 |
52047584 |
- |
NID2 |
NNNAGCACTTCTTACCACCA |
NGG |
1 |
0.6 |
Tier II |
| 4 |
NC_000021.9 |
21 |
37719489 |
+ |
KCNJ6 |
NNNGCAACTTCTTACCACCA |
NGG |
2 |
0.5804 |
Tier II |
| 5 |
NC_000023.11 |
X |
148959795 |
- |
AFF2 |
NNNACCACGTCTTACCATCA |
NGG |
2 |
0.398 |
Tier II |
| 6 |
NC_000011.10 |
11 |
126901363 |
- |
KIRREL3 |
NNNACTACTTCTTACCACAA |
NGG |
2 |
0.398 |
Tier II |
| 7 |
NC_000001.11 |
1 |
30936161 |
+ |
PUM1 |
NNNACCACTGCTCACCACCA |
NGG |
2 |
0.3947 |
Tier II |
| 8 |
NC_000001.11 |
1 |
30949590 |
+ |
PUM1 |
NNNACCACTGCTCACCACCA |
NGG |
2 |
0.3947 |
Tier II |
| 9 |
NC_000012.12 |
12 |
99543287 |
+ |
ANKS1B |
NNNACCACTTCTCACCACTA |
NGG |
2 |
0.3644 |
Tier II |
| 10 |
NC_000006.12 |
6 |
42173210 |
- |
GUCA1ANB-GUCA1A |
NNNATCCCTTCTTACCACCA |
NGG |
2 |
0.2784 |
Tier II |
| 11 |
NC_000013.11 |
13 |
51625193 |
- |
WDFY2 |
NNNACCACCTCTTACCACCC |
NGG |
2 |
0.2098 |
Tier II |
| 12 |
NC_000001.11 |
1 |
10956212 |
- |
C1orf127 |
NNNACCACTTCCTACCTCCA |
NGG |
2 |
0.1263 |
Tier II |
| 13 |
NC_000006.12 |
6 |
16383972 |
+ |
ATXN1 |
NNNGCCACTTCTTACCAGCA |
NGG |
2 |
0.0833 |
Tier II |
| 14 |
NC_000023.11 |
X |
24627780 |
- |
PCYT1B |
NNNACCACTTCTTACCACCT |
NCG |
2 |
0.0643 |
Tier II |
| 15 |
NC_000010.11 |
10 |
102710759 |
- |
ARL3 |
NNNACCACTTCTGCCCACCA |
NGG |
2 |
0.0559 |
Tier II |
| 16 |
NC_000016.10 |
16 |
70541150 |
- |
SF3B3 |
NNNACCACTTCATACCACCA |
NTG |
2 |
0.0312 |
Tier II |
| 17 |
NC_000023.11 |
X |
66197010 |
+ |
HEPH |
NNNACCACTTCTTACCATCA |
NGC |
2 |
0.0143 |
Tier II |
| 18 |
NC_000016.10 |
16 |
87971052 |
+ |
BANP |
NNNACCACTTCTTACCGCCA |
NTG |
2 |
0.0069 |
Tier II |
| 19 |
NC_000006.12 |
6 |
39588842 |
+ |
KIF6 |
NNNACCACTTCTTACCACCA |
NCA |
2 |
0.0 |
Tier II |
| 20 |
NC_000007.14 |
7 |
137724500 |
- |
DGKI |
NNNACCACTTCTTACCACCA |
NCA |
2 |
0.0 |
Tier II |
| 21 |
NC_000016.10 |
16 |
73337019 |
+ |
ZFHX3 |
NNNACCACTTCTTACCACCA |
NTA |
2 |
0.0 |
Tier II |
| 22 |
NC_000021.9 |
21 |
37719489 |
+ |
KCNJ6-AS1 |
NNNGCAACTTCTTACCACCA |
NGG |
2 |
0.5804 |
Tier III |
| 23 |
NC_000011.10 |
11 |
126901363 |
- |
LOC105369559 |
NNNACTACTTCTTACCACAA |
NGG |
2 |
0.398 |
Tier III |
| 24 |
NC_000002.12 |
2 |
58675614 |
- |
LINC01122 |
NNNACCACTTCTTACCACAA |
NTG |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
9397487 |
+ |
Elapor2 |
NNNTCCAGTTCTTACCACCA |
NGG |
2 |
0.4091 |
Tier II |
| 2 |
NC_000073.6 |
7 |
101403916 |
- |
Arap1 |
NNNACCACTTAGTACCACCA |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000080.6 |
14 |
60205455 |
- |
Nupl1 |
NNNCCCATTTCTTACCACCA |
NGG |
2 |
0.3088 |
Tier II |
| 4 |
NC_000069.6 |
3 |
104911555 |
- |
St7l |
NNNACCTCTTCTTACCAACA |
NGG |
2 |
0.2356 |
Tier II |
| 5 |
NC_000078.6 |
12 |
112007157 |
- |
Tdrd9 |
NNNAACACTTCTTACTACCA |
NGG |
2 |
0.1758 |
Tier II |
| 6 |
NC_000069.6 |
3 |
121306857 |
- |
Alg14 |
NNNACCTCTTCTGACCACCA |
NGG |
2 |
0.1141 |
Tier II |
| 7 |
NC_000071.6 |
5 |
139086555 |
+ |
Prkar1b |
NNNACCACTTCTTACCACTC |
NGG |
2 |
0.1049 |
Tier II |
| 8 |
NC_000077.6 |
11 |
108235493 |
- |
Prkca |
NNNACCACTTCCTATCACCA |
NGG |
2 |
0.0632 |
Tier II |
| 9 |
NC_000073.6 |
7 |
55894381 |
- |
Cyfip1 |
NNNACCACTTCTTGCCTCCA |
NGG |
2 |
0.0622 |
Tier II |
| 10 |
NC_000081.6 |
15 |
58542645 |
+ |
Fer1l6 |
NNNACCCCTTCTTACCAGCA |
NGG |
2 |
0.0583 |
Tier II |
| 11 |
NC_000068.7 |
2 |
109118980 |
+ |
Mettl15 |
NNNACCACTTCATACCACCA |
NTG |
2 |
0.0312 |
Tier II |
| 12 |
NC_000076.6 |
10 |
88389613 |
+ |
Gnptab |
NNNACCACTCCTTACCACCA |
NGC |
2 |
0.0119 |
Tier II |
| 13 |
NC_000076.6 |
10 |
88502899 |
+ |
Chpt1 |
NNNACCACTTCTGACCACCA |
NTG |
2 |
0.0102 |
Tier II |
| 14 |
NC_000073.6 |
7 |
51609405 |
+ |
Gm33136 |
NNNACCCCTTCTTACCACCA |
NGG |
1 |
0.4375 |
Tier III |
| 15 |
NC_000073.6 |
7 |
44676367 |
- |
2310016G11Rik |
NNNCCCACTTCTCACCACCA |
NGG |
2 |
0.2786 |
Tier III |
| 16 |
NC_000081.6 |
15 |
57629546 |
+ |
Gm35682 |
NNNTCCCCTTCTTACCACCA |
NGG |
2 |
0.2784 |
Tier III |
| 17 |
NC_000072.6 |
6 |
48997587 |
+ |
4930563H07Rik |
NNNACCACTTCCTACCACCC |
NGG |
2 |
0.2153 |
Tier III |
| 18 |
NC_000072.6 |
6 |
48997587 |
+ |
Gm19173 |
NNNACCACTTCCTACCACCC |
NGG |
2 |
0.2153 |
Tier III |
| 19 |
NC_000074.6 |
8 |
126418991 |
+ |
Gm51604 |
NNNACCACTTCTTAACACCA |
NTG |
2 |
0.0087 |
Tier III |
Other clones with same target sequence:
(none)
