Construct: sgRNA BRDN0001162290
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCCTTCCTAGGCGATCCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DAPK3 (1613)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77557
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
3959632 |
- |
DAPK3 |
NNNCTTCCTAGGCGATCCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
131143044 |
- |
TCERG1L |
NNNCTTCCTAGGGGATCCAG |
NGG |
2 |
0.0974 |
Tier II |
| 3 |
NC_000004.12 |
4 |
92442214 |
+ |
GRID2 |
NNNCTTCCGAGGCGATCGGG |
NGG |
2 |
0.0825 |
Tier II |
| 4 |
NC_000008.11 |
8 |
55286505 |
+ |
XKR4 |
NNNCTTCCTAGGGGATACGG |
NGG |
2 |
0.0636 |
Tier II |
| 5 |
NC_000012.12 |
12 |
103225928 |
+ |
C12orf42 |
NNNCTTCCTAGGCGATCGGG |
NAG |
2 |
0.0346 |
Tier II |
| 6 |
NC_000010.11 |
10 |
6632846 |
+ |
LINC02648 |
NNNCTTCCGAGGCGATCCGG |
NAG |
2 |
0.1605 |
Tier III |
| 7 |
NC_000010.11 |
10 |
6800343 |
+ |
LINC00707 |
NNNCTTCCGAGGCGATCCGG |
NAG |
2 |
0.1605 |
Tier III |
| 8 |
NC_000012.12 |
12 |
10336177 |
+ |
LINC02617 |
NNNCTTCCGAGGCGATCCGG |
NAG |
2 |
0.1605 |
Tier III |
| 9 |
NC_000019.10 |
19 |
44539349 |
+ |
CEACAM22P |
NNNCTTCCTAGGCGAGCCCG |
NGG |
2 |
0.1552 |
Tier III |
| 10 |
NC_000023.11 |
X |
116815736 |
+ |
LOC100126447 |
NNNCTTCCGAGGCGATCGGG |
NGG |
2 |
0.0825 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
150693802 |
+ |
Hmcn1 |
NNNCTTCCTAGGCGATCCCG |
NGT |
2 |
0.0072 |
Tier II |
| 2 |
NC_000069.6 |
3 |
58818073 |
- |
Mindy4b-ps |
NNNCTTCCTAGGCCATCCAG |
NGG |
2 |
0.3061 |
Tier III |
Other clones with same target sequence:
(none)
