Construct: sgRNA BRDN0001162291
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCAGCGTGCCCATCCCGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRIM33 (51592)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77097
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
114510580 |
- |
TRIM33 |
NNNAGCGTGCCCATCCCGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
18854472 |
- |
TAS1R2 |
NNNAGCGTGCCCATCCCGGG |
NTT |
2 |
0.0 |
Tier I |
| 3 |
NC_000001.11 |
1 |
3477468 |
+ |
ARHGEF16 |
NNNAGCGTGGCCATCACGGG |
NGG |
2 |
0.3889 |
Tier II |
| 4 |
NC_000017.11 |
17 |
79837712 |
+ |
CBX4 |
NNNAGCGTGCCCATCCAGGC |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000023.11 |
X |
153577191 |
- |
ATP2B3 |
NNNAGCGTGCCCCTCCCGGA |
NGG |
2 |
0.1974 |
Tier II |
| 6 |
NC_000001.11 |
1 |
22743774 |
+ |
EPHB2 |
NNNACCGTGCCCAGCCCGGG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000017.11 |
17 |
1162496 |
- |
ABR |
NNNAGCGTGCACAGCCCGGG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000018.10 |
18 |
59273338 |
- |
RAX |
NNNAGCGTGCGCAGCCCGGG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000007.14 |
7 |
24025288 |
- |
LOC105375188 |
NNNAGCATGCTCATCCCGGG |
NGG |
2 |
0.3077 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
103280418 |
+ |
Trim33 |
NNNAGCGTGCCCATCCCTGG |
NGG |
1 |
0.6667 |
Tier I |
| 2 |
NC_000084.6 |
18 |
57878856 |
- |
Slc12a2 |
NNNCGCGTCCCCATCCCGGG |
NGG |
2 |
0.19 |
Tier I |
| 3 |
NC_000081.6 |
15 |
100488093 |
- |
Csrnp2 |
NNNAGCGTGCCCAGCCAGGG |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000074.6 |
8 |
109821517 |
+ |
Ap1g1 |
NNNAGGGTGCCCGTCCCGGG |
NGG |
2 |
0.3261 |
Tier II |
Other clones with same target sequence:
(none)
