Construct: sgRNA BRDN0001162300
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTGAATGCCGGAAGCAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PLXNA4 (91584)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76878
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
132187514 |
- |
PLXNA4 |
NNNGAATGCCGGAAGCAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
92114046 |
+ |
SLCO3A1 |
NNNGAATGCCAGAAGCAATG |
NGG |
2 |
0.4615 |
Tier II |
| 3 |
NC_000004.12 |
4 |
7728501 |
- |
SORCS2 |
NNNGGATGCCGGAAGCACCG |
NGG |
2 |
0.1371 |
Tier II |
| 4 |
NC_000007.14 |
7 |
94309603 |
- |
LOC112267858 |
NNNGAGTGCCGGAATCAACG |
NGG |
2 |
0.102 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
50842745 |
- |
Alg9 |
NNNGAATGCCCGAAGCAACT |
NGG |
2 |
0.3 |
Tier I |
| 2 |
NC_000071.6 |
5 |
67666297 |
- |
Atp8a1 |
NNNGAATGCCAGAAGCAAAG |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000071.6 |
5 |
124425257 |
+ |
Sbno1 |
NNNGAACGCCGGAACCAACG |
NGG |
2 |
0.1875 |
Tier II |
| 4 |
NC_000075.6 |
9 |
50676470 |
+ |
Dixdc1 |
NNNGAATGCCTGAAGCCACG |
NGG |
2 |
0.0679 |
Tier II |
| 5 |
NC_000077.6 |
11 |
114236499 |
- |
Gm11690 |
NNNGAATGTGGGAAGCAACG |
NGG |
2 |
0.3403 |
Tier III |
| 6 |
NC_000081.6 |
15 |
88483244 |
- |
Gm35831 |
NNNGGATGCCGGAAGCAACC |
NGG |
2 |
0.3086 |
Tier III |
Other clones with same target sequence:
(none)
