Construct: sgRNA BRDN0001162304
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCTCCCTGGAACAAGACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERN1 (2081)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76407
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
64055683 |
- |
ERN1 |
NNNTCCCTGGAACAAGACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
41696853 |
+ |
MGA |
NNNTCCCTGGAAAAAGAGGA |
NGG |
2 |
0.0513 |
Tier I |
| 3 |
NC_000007.14 |
7 |
32368082 |
+ |
PDE1C |
NNNTGCCTGGAACAAGACAA |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000006.12 |
6 |
12235100 |
- |
EDN1 |
NNNTCACTGGAACAAGACCA |
NGG |
2 |
0.4163 |
Tier II |
| 5 |
NC_000009.12 |
9 |
84720781 |
+ |
NTRK2 |
NNNTCCATGGAACAAGAAGA |
NGG |
2 |
0.4038 |
Tier II |
| 6 |
NC_000022.11 |
22 |
29297816 |
- |
EWSR1 |
NNNTCCCTGGAACAAGACAA |
NAG |
2 |
0.1852 |
Tier II |
| 7 |
NC_000009.12 |
9 |
76026403 |
+ |
PCSK5 |
NNNTACCTGGAACCAGACGA |
NGG |
2 |
0.1224 |
Tier II |
| 8 |
NC_000009.12 |
9 |
109772283 |
- |
PALM2AKAP2 |
NNNTCCCTGGAAGAAGACGG |
NGG |
2 |
0.1043 |
Tier II |
| 9 |
NC_000023.11 |
X |
43807365 |
- |
MAOB |
NNNTTCCTGGAAGAAGACGA |
NGG |
2 |
0.0868 |
Tier II |
| 10 |
NC_000005.10 |
5 |
150371995 |
- |
TCOF1 |
NNNCCCCTGGAACAAGAGGA |
NGG |
2 |
0.0863 |
Tier II |
| 11 |
NC_000007.14 |
7 |
96664247 |
+ |
SEM1 |
NNNTCCCTGGAACAACAGGA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000012.12 |
12 |
132463113 |
- |
LOC124903060 |
NNNTCTCTGGAACAAGACTA |
NGG |
2 |
0.619 |
Tier III |
| 13 |
NC_000015.10 |
15 |
40047699 |
+ |
SRP14-DT |
NNNTCCCTGGAACTGGACGA |
NGG |
2 |
0.3467 |
Tier III |
| 14 |
NC_000006.12 |
6 |
19810515 |
- |
LOC105374963 |
NNNTGCCTGGAACAAGGCGA |
NGG |
2 |
0.1059 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
121302127 |
+ |
Map1a |
NNNTCCCTGGAAGAAGACAA |
NGG |
2 |
0.0974 |
Tier I |
| 2 |
NC_000081.6 |
15 |
39659919 |
+ |
Rims2 |
NNNTCCCTGGAAGAAGATGA |
NGG |
2 |
0.0877 |
Tier I |
| 3 |
NC_000075.6 |
9 |
73628493 |
- |
Unc13c |
NNNTTCCTGGAACAAGACTA |
NGG |
2 |
0.4242 |
Tier II |
| 4 |
NC_000068.7 |
2 |
155708256 |
+ |
Edem2 |
NNNTCCCTGGAGCAAGAGGA |
NGG |
2 |
0.0963 |
Tier II |
| 5 |
NC_000083.6 |
17 |
32238808 |
- |
Brd4 |
NNNTCCCTGGGACAAGAGGA |
NGG |
2 |
0.0867 |
Tier II |
| 6 |
NC_000083.6 |
17 |
56371029 |
+ |
Kdm4b |
NNNTCCCTGGAACCAGAGGA |
NGG |
2 |
0.0286 |
Tier II |
| 7 |
NC_000070.6 |
4 |
3672837 |
- |
Gm42250 |
NNNTGCCTGGAGCAAGACGA |
NGG |
2 |
0.4333 |
Tier III |
Other clones with same target sequence:
(none)
