Construct: sgRNA BRDN0001162316
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAATGGAGCAAGCCATCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKD1 (5587)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77229
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
29927277 |
+ |
PRKD1 |
NNNTGGAGCAAGCCATCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
57108721 |
- |
CPNE2 |
NNNTGGAGCAACCCATCTCA |
NGG |
2 |
0.4963 |
Tier II |
| 3 |
NC_000003.12 |
3 |
37611372 |
+ |
ITGA9 |
NNNAGGAGCAAGCCATCTCC |
NGG |
2 |
0.2041 |
Tier II |
| 4 |
NC_000012.12 |
12 |
62233652 |
- |
TAFA2 |
NNNTGGAGCAGGCCATCTCG |
NGT |
2 |
0.0105 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
50648744 |
+ |
Prkd1 |
NNNTGGAACAAGCCATCTCC |
NGG |
2 |
0.4286 |
Tier I |
| 2 |
NC_000085.6 |
19 |
41559053 |
+ |
Lcor |
NNNTGGAGGAAGCCATCTCG |
NTG |
2 |
0.0241 |
Tier I |
| 3 |
NC_000071.6 |
5 |
115177808 |
- |
Cabp1 |
NNNTAGAACAAGCCATCTCG |
NGG |
2 |
0.8667 |
Tier II |
| 4 |
NC_000080.6 |
14 |
121488848 |
- |
Slc15a1 |
NNNAGGAGCAAGCCATCTCG |
NGG |
1 |
0.4762 |
Tier II |
| 5 |
NC_000078.6 |
12 |
51411748 |
- |
Scfd1 |
NNNTGGTGCAAACCATCTCG |
NGG |
2 |
0.4083 |
Tier II |
| 6 |
NC_000074.6 |
8 |
126453043 |
+ |
Tarbp1 |
NNNTGGAGCAAGTCAGCTCG |
NGG |
2 |
0.2423 |
Tier II |
| 7 |
NC_000085.6 |
19 |
37009826 |
- |
Btaf1 |
NNNTGGAGGAAGCCATCTCG |
NTG |
2 |
0.0241 |
Tier II |
| 8 |
NC_000073.6 |
7 |
61826445 |
+ |
A230057D06Rik |
NNNTGGATCAAGCCATCTCA |
NGG |
2 |
0.5859 |
Tier III |
Other clones with same target sequence:
(none)
