Construct: sgRNA BRDN0001162330

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACATATACAGTAGAGACCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK9 (221264)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77690

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 109659336 - AK9 NNNTATACAGTAGAGACCAG NGG 0 1.0 Tier I
2 NC_000003.12 3 122249090 - CASR NNNTACACAGTAAAGACCAG NGG 2 0.8392 Tier II
3 NC_000023.11 X 75160386 - UPRT NNNTATACAATAGAGACAAG NGG 2 0.5026 Tier II
4 NC_000004.12 4 44349934 + KCTD8 NNNTAGATAGTAGAGACCAG NGG 2 0.5 Tier II
5 NC_000002.12 2 124852810 + CNTNAP5 NNNTATTTAGTAGAGACCAG NGG 2 0.3828 Tier II
6 NC_000023.11 X 149623429 + TMEM185A NNNTAATCAGTAGAGACCAG NGG 2 0.3792 Tier II
7 NC_000005.10 5 90584524 - ADGRV1 NNNTATACAGCAGAGACAAG NGG 2 0.359 Tier II
8 NC_000005.10 5 139183502 + SIL1 NNNTATTCAGAAGAGACCAG NGG 2 0.3281 Tier II
9 NC_000005.10 5 81667461 + SSBP2 NNNCATTCAGTAGAGACCAG NGG 2 0.2831 Tier II
10 NC_000002.12 2 23401198 + KLHL29 NNNTCTACAGTAGAGACCTG NGG 2 0.2692 Tier II
11 NC_000005.10 5 14714890 - ANKH NNNTCTACAGTAGTGACCAG NGG 2 0.2667 Tier II
12 NC_000005.10 5 14714890 - OTULIN NNNTCTACAGTAGTGACCAG NGG 2 0.2667 Tier II
13 NC_000003.12 3 27193329 - NEK10 NNNTTTACAGTACAGACCAG NGG 2 0.1531 Tier II
14 NC_000006.12 6 136137011 + PDE7B NNNTATACACTAGATACCAG NGG 2 0.0571 Tier II
15 NC_000011.10 11 106083392 - AASDHPPT NNNTATACTGTAGAGAGCAG NGG 2 0.0353 Tier II
16 NC_000003.12 3 124641517 - KALRN NNNTATACAGTAGATAGCAG NGG 2 0.0084 Tier II
17 NC_000003.12 3 11579045 - VGLL4 NNNTATACAGTAGAGGCCAG NTG 2 0.0075 Tier II
18 NC_000001.11 1 33981625 + CSMD2 NNNTATACAGTAGAGACCAG NAC 2 0.0 Tier II
19 NC_000009.12 9 104130169 - SMC2 NNNTATACAGTAGAGTCAAG NGG 2 0.0 Tier II
20 NC_000022.11 22 36681844 + CACNG2 NNNTATACAGTAGATCCCAG NGG 2 0.0 Tier II
21 NC_000020.11 20 23333034 - LOC105372571 NNNTATAAAGTAGAGACCAA NGG 2 0.6094 Tier III
22 NC_000020.11 20 23333034 - NXT1-AS1 NNNTATAAAGTAGAGACCAA NGG 2 0.6094 Tier III
23 NC_000005.10 5 14714890 - LOC100130744 NNNTCTACAGTAGTGACCAG NGG 2 0.2667 Tier III
24 NC_000004.12 4 96530934 + LINC02267 NNNTATACAGTAGAGTCAAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 177142248 - Akt3 NNNTAAACAGTAGAGACCAA NGG 2 0.8125 Tier II
2 NC_000071.6 5 3457210 + Cdk6 NNNTGTACAGTAAAGACCAG NGG 2 0.6646 Tier II
3 NC_000076.6 10 122800438 + Ppm1h NNNCATACAGTAGAGACCAG NGG 1 0.6471 Tier II
4 NC_000067.6 1 174789196 + Fmn2 NNNTATAGAGTAGAGACCAA NGG 2 0.6027 Tier II
5 NC_000085.6 19 16533258 + Gna14 NNNTGTACATTAGAGACCAG NGG 2 0.585 Tier II
6 NC_000086.7 X 100514300 + Igbp1 NNNTAAACAGCAGAGACCAG NGG 2 0.5778 Tier II
7 NC_000080.6 14 115856099 - Gpc5 NNNTATGGAGTAGAGACCAG NGG 2 0.4538 Tier II
8 NC_000075.6 9 9751349 + Cntn5 NNNTATGCAGTAGAGACAAG NGG 2 0.3801 Tier II
9 NC_000081.6 15 39842580 - Dpys NNNCAGACAGTAGAGACCAG NGG 2 0.3697 Tier II
10 NC_000073.6 7 90650931 - Dlg2 NNNGCTACAGTAGAGACCAG NGG 2 0.3125 Tier II
11 NC_000069.6 3 32354991 - Zmat3 NNNAATACAGTAGAGAACAG NGG 2 0.2222 Tier II
12 NC_000071.6 5 100041366 + Enoph1 NNNATTACAGTAGAGACCAG NGG 2 0.1732 Tier II
13 NC_000069.6 3 133044240 + Gstcd NNNTATACAGGAGAGACCAC NGG 2 0.1714 Tier II
14 NC_000070.6 4 95847487 - Fggy NNNTAGACAGTAGAGGCCAG NGG 2 0.1099 Tier II
15 NC_000067.6 1 21485241 - Kcnq5 NNNTATACAGTAGAGGCCAG NAG 2 0.0499 Tier II
16 NC_000067.6 1 84243858 - Pid1 NNNTATACAGAAGAGAGCAG NGG 2 0.0441 Tier II
17 NC_000073.6 7 27925569 + Zfp974 NNNTATACAGTAGAGAACAG NGT 2 0.0075 Tier II
18 NC_000067.6 1 130047085 - Thsd7b NNNTGTACAGTAGAGTCCAG NGG 2 0.0 Tier II
19 NC_000072.6 6 3311040 + Gm8579 NNNTGTACAGTAAAGACCAG NGG 2 0.6646 Tier III
20 NC_000074.6 8 3089401 + Gm30220 NNNTGTACAGTAAAGACCAG NGG 2 0.6646 Tier III
21 NC_000079.6 13 3117319 + Gm17177 NNNTGTACAGTAAAGACCAG NGG 2 0.6646 Tier III
22 NC_000079.6 13 3117319 + Gm46400 NNNTGTACAGTAAAGACCAG NGG 2 0.6646 Tier III
23 NC_000077.6 11 52706850 - Gm39823 NNNTATACAGTAGAGACAAA NGG 2 0.5048 Tier III
24 NC_000067.6 1 121119500 - Celrr NNNTATACAATAGATACCAG NGG 2 0.1333 Tier III
25 NC_000067.6 1 121119500 - Gm46155 NNNTATACAATAGATACCAG NGG 2 0.1333 Tier III
26 NC_000067.6 1 106164658 + Gm20753 NNNTATACTGTAGAGAGCAG NGG 2 0.0353 Tier III
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Other clones with same target sequence:

(none)