Construct: sgRNA BRDN0001162335
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTCAGATGATAACTTCAACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK10 (152110)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75636
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
27174671 |
- |
NEK10 |
NNNAGATGATAACTTCAACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
51701215 |
+ |
SCG3 |
NNNAGATGATAACTCCAACC |
NAG |
2 |
0.0707 |
Tier I |
| 3 |
NC_000023.11 |
X |
71403126 |
- |
TAF1 |
NNNAGAGGATAACTTAAACC |
NGG |
2 |
0.5882 |
Tier II |
| 4 |
NC_000012.12 |
12 |
119193358 |
+ |
HSPB8 |
NNNAGGTGATGACTTCAACC |
NGG |
2 |
0.4643 |
Tier II |
| 5 |
NC_000003.12 |
3 |
109096057 |
+ |
MORC1 |
NNNAGATGAAAACTTCAACA |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000023.11 |
X |
16806408 |
- |
TXLNG |
NNNAGTTGTTAACTTCAACC |
NGG |
2 |
0.4286 |
Tier II |
| 7 |
NC_000003.12 |
3 |
62148067 |
+ |
PTPRG |
NNNAGATAATCACTTCAACC |
NGG |
2 |
0.4 |
Tier II |
| 8 |
NC_000018.10 |
18 |
76892057 |
+ |
ZNF236 |
NNNAGATGATAAATTAAACC |
NGG |
2 |
0.3846 |
Tier II |
| 9 |
NC_000013.11 |
13 |
27567307 |
+ |
LNX2 |
NNNAGATGATAATTTCAACA |
NGG |
2 |
0.35 |
Tier II |
| 10 |
NC_000007.14 |
7 |
111154855 |
- |
IMMP2L |
NNNAGAGGATCACTTCAACC |
NGG |
2 |
0.2353 |
Tier II |
| 11 |
NC_000019.10 |
19 |
48829286 |
- |
HSD17B14 |
NNNAGAGGATCACTTCAACC |
NGG |
2 |
0.2353 |
Tier II |
| 12 |
NC_000002.12 |
2 |
11818798 |
- |
LPIN1 |
NNNAGATGAGAACTCCAACC |
NGG |
2 |
0.1364 |
Tier II |
| 13 |
NC_000021.9 |
21 |
42489911 |
- |
RSPH1 |
NNNACATGATAACTTGAACC |
NGG |
2 |
0.1209 |
Tier II |
| 14 |
NC_000002.12 |
2 |
159330779 |
+ |
BAZ2B |
NNNAGAGGATAACTTGAACC |
NGG |
2 |
0.0905 |
Tier II |
| 15 |
NC_000017.11 |
17 |
80235454 |
- |
SLC26A11 |
NNNAGATGATAACTTGAGCC |
NGG |
2 |
0.0615 |
Tier II |
| 16 |
NC_000004.12 |
4 |
140937358 |
- |
RNF150 |
NNNAGATGATCACTTGAACC |
NGG |
2 |
0.0615 |
Tier II |
| 17 |
NC_000023.11 |
X |
97227998 |
- |
DIAPH2 |
NNNAGATGATCACTTGAACC |
NGG |
2 |
0.0615 |
Tier II |
| 18 |
NC_000001.11 |
1 |
89153697 |
+ |
GBP7 |
NNNAGATGATAACTTCTGCC |
NGG |
2 |
0.0533 |
Tier II |
| 19 |
NC_000012.12 |
12 |
94410403 |
- |
CEP83 |
NNNAGATGATAACTTCAACA |
NGT |
2 |
0.0081 |
Tier II |
| 20 |
NC_000008.11 |
8 |
127994952 |
- |
PVT1 |
NNNAGATTATCACTTCAACC |
NGG |
2 |
0.25 |
Tier III |
| 21 |
NC_000005.10 |
5 |
109294825 |
+ |
LOC285638 |
NNNAGCTGATAACTTCAACC |
NAG |
2 |
0.1178 |
Tier III |
| 22 |
NC_000001.11 |
1 |
89153697 |
+ |
LOC105378842 |
NNNAGATGATAACTTCTGCC |
NGG |
2 |
0.0533 |
Tier III |
| 23 |
NC_000022.11 |
22 |
34665375 |
+ |
LOC105373013 |
NNNAGATGATAAAGTCAACC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
75661283 |
- |
Scg3 |
NNNAGATGATAACTCCAACC |
NAG |
2 |
0.0707 |
Tier I |
| 2 |
NC_000076.6 |
10 |
20610756 |
+ |
Pde7b |
NNNAGTTGCTAACTTCAACC |
NGG |
2 |
0.4082 |
Tier II |
| 3 |
NC_000080.6 |
14 |
93306132 |
+ |
Pcdh9 |
NNNAGATAATAAATTCAACC |
NGG |
2 |
0.3846 |
Tier II |
| 4 |
NC_000071.6 |
5 |
50041190 |
+ |
Adgra3 |
NNNAGATGAGAAATTCAACC |
NGG |
2 |
0.1923 |
Tier II |
| 5 |
NC_000077.6 |
11 |
50203865 |
- |
Sqstm1 |
NNNAGATGAGAACTTGAACC |
NGG |
2 |
0.0769 |
Tier II |
| 6 |
NC_000083.6 |
17 |
67114923 |
- |
Ptprm |
NNNAGATGATAACTGCAGCC |
NGG |
2 |
0.02 |
Tier II |
| 7 |
NC_000072.6 |
6 |
5106299 |
+ |
Ppp1r9a |
NNNAGATGATAACTTCACCC |
NTG |
2 |
0.0074 |
Tier II |
| 8 |
NC_000067.6 |
1 |
175306352 |
- |
Rgs7 |
NNNAGATGATAACTTCCACC |
NTG |
2 |
0.0069 |
Tier II |
| 9 |
NC_000081.6 |
15 |
101023580 |
- |
Scn8a |
NNNAGATGATAACTTCCACC |
NTG |
2 |
0.0069 |
Tier II |
| 10 |
NC_000071.6 |
5 |
50041190 |
+ |
LOC115490186 |
NNNAGATGAGAAATTCAACC |
NGG |
2 |
0.1923 |
Tier III |
Other clones with same target sequence:
(none)
