Construct: sgRNA BRDN0001162341
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTATTCCGAAGTCCTCCGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80200
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
79521501 |
- |
MAF |
NNNATTCCGAAGTCCACCAC |
NGG |
2 |
0.6494 |
Tier II |
| 2 |
NC_000009.12 |
9 |
128798313 |
+ |
TBC1D13 |
NNNATGCCGAAGTCCTCAGC |
NGG |
2 |
0.3077 |
Tier II |
| 3 |
NC_000015.10 |
15 |
25225333 |
+ |
SNHG14 |
NNNATACCGAAGTCCCCCGC |
NGG |
2 |
0.5778 |
Tier III |
| 4 |
NC_000017.11 |
17 |
76240995 |
- |
LOC105371896 |
NNNTTTCCGAAGACCTCCGC |
NGG |
2 |
0.4406 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
13842338 |
- |
Runx1t1 |
NNNATTCCCAATTCCTCCGC |
NGG |
2 |
0.2071 |
Tier II |
| 2 |
NC_000079.6 |
13 |
45671781 |
- |
Atxn1 |
NNNATTCCGAAGTCCTCCTT |
NGG |
2 |
0.2 |
Tier II |
Other clones with same target sequence:
(none)
