Construct: sgRNA BRDN0001162353
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCGGGGCTTTACCAGTAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP4K4 (9448)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76266
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
101859078 |
- |
MAP4K4 |
NNNGGGCTTTACCAGTAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
148781646 |
+ |
EPC2 |
NNNGTTCTTTACCAGTAACA |
NGG |
2 |
0.2 |
Tier I |
| 3 |
NC_000007.14 |
7 |
78476756 |
+ |
MAGI2 |
NNNGGTCTTTAACAGTAACA |
NGG |
2 |
0.4762 |
Tier II |
| 4 |
NC_000011.10 |
11 |
12433826 |
+ |
PARVA |
NNNGGTCTTTAACAGTAACA |
NGG |
2 |
0.4762 |
Tier II |
| 5 |
NC_000004.12 |
4 |
5320694 |
- |
STK32B |
NNNGGGCTTTTGCAGTAACA |
NGG |
2 |
0.1368 |
Tier II |
| 6 |
NC_000020.11 |
20 |
23442806 |
+ |
CSTL1 |
NNNGGGCTTGACCAGTGACA |
NGG |
2 |
0.0882 |
Tier II |
| 7 |
NC_000006.12 |
6 |
122407005 |
- |
HSF2 |
NNNTGGCTTTACCAGTTACA |
NGG |
2 |
0.0485 |
Tier II |
| 8 |
NC_000002.12 |
2 |
111208559 |
+ |
MIR4435-2HG |
NNNGGGCTTTATCAGTAACA |
NGG |
1 |
0.5385 |
Tier III |
| 9 |
NC_000003.12 |
3 |
65181467 |
+ |
LINC02040 |
NNNGGGCTGTCCCAGTAACA |
NGG |
2 |
0.2476 |
Tier III |
| 10 |
NC_000009.12 |
9 |
21514586 |
- |
MIR31HG |
NNNGGGTTTTACCAGTAACC |
NGG |
2 |
0.1847 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
39999970 |
- |
Map4k4 |
NNNGGGCGTTACCAGTAACA |
NGG |
1 |
0.7333 |
Tier I |
| 2 |
NC_000067.6 |
1 |
92125307 |
+ |
Hdac4 |
NNNGGGCTTTACCTGAAACA |
NGG |
2 |
0.4848 |
Tier II |
| 3 |
NC_000079.6 |
13 |
58100804 |
- |
Klhl3 |
NNNGGGCTTGACTAGTAACA |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000083.6 |
17 |
71689766 |
+ |
Togaram2 |
NNNGGGCTTTACCAGGAACA |
NGG |
1 |
0.3462 |
Tier II |
| 5 |
NC_000071.6 |
5 |
102431963 |
- |
Arhgap24 |
NNNTGGCTTCACCAGTAACA |
NGG |
2 |
0.1939 |
Tier II |
| 6 |
NC_000082.6 |
16 |
87430044 |
- |
Ltn1 |
NNNGGGCTGTACCACTAACA |
NGG |
2 |
0.1688 |
Tier II |
| 7 |
NC_000067.6 |
1 |
118954628 |
- |
Gli2 |
NNNGGGCTTTACCAGTAGCC |
NGG |
2 |
0.0909 |
Tier II |
| 8 |
NC_000082.6 |
16 |
5114571 |
- |
Ppl |
NNNGGGCTTTAGCATTAACA |
NGG |
2 |
0.0635 |
Tier II |
| 9 |
NC_000071.6 |
5 |
34498235 |
- |
Tnip2 |
NNNGGGCTTTAGCAGTAACA |
NGA |
2 |
0.0309 |
Tier II |
| 10 |
NC_000080.6 |
14 |
75522421 |
- |
Siah3 |
NNNGGGCTTTACTAGTAACA |
NGC |
2 |
0.0156 |
Tier II |
| 11 |
NC_000084.6 |
18 |
21168610 |
+ |
Garem1 |
NNNGGGCTTTACCACTAACA |
NTG |
2 |
0.0106 |
Tier II |
| 12 |
NC_000069.6 |
3 |
52793903 |
- |
Gm30292 |
NNNGGGCCTTAGCAGTAACA |
NGG |
2 |
0.4444 |
Tier III |
| 13 |
NC_000079.6 |
13 |
58100804 |
- |
Gm33389 |
NNNGGGCTTGACTAGTAACA |
NGG |
2 |
0.35 |
Tier III |
| 14 |
NC_000082.6 |
16 |
87028967 |
+ |
Gm38477 |
NNNTGGCTTTACCAGAAACA |
NGG |
2 |
0.3306 |
Tier III |
| 15 |
NC_000067.6 |
1 |
118954628 |
- |
Gm51642 |
NNNGGGCTTTACCAGTAGCC |
NGG |
2 |
0.0909 |
Tier III |
| 16 |
NC_000080.6 |
14 |
75522421 |
- |
Gm35966 |
NNNGGGCTTTACTAGTAACA |
NGC |
2 |
0.0156 |
Tier III |
Other clones with same target sequence:
(none)
