Construct: sgRNA BRDN0001162354

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCGGCCAGTTTGCGATCGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAPK3 (1613)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77556

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 3964976 - DAPK3 NNNGCCAGTTTGCGATCGTG NGG 0 1.0 Tier I
2 NC_000015.10 15 63983739 - DAPK2 NNNGCCAGTTTGCCATCGTG NAG 2 0.1111 Tier I
3 NC_000016.10 16 9757578 - GRIN2A NNNGCCAGTTTGGGATTGTG NGG 2 0.0636 Tier II
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 66220550 + Dapk2 NNNGCCAGTTTGCCATCGTG NAG 2 0.1111 Tier I
Download CSV

Other clones with same target sequence:

(none)