Construct: sgRNA BRDN0001162355
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCGGACTTCCTCGAGTACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HK2 (3099)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76311
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
74882164 |
+ |
HK2 |
NNNGACTTCCTCGAGTACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
69250577 |
+ |
HKDC1 |
NNNGACTTCCTGGACTACAT |
NGG |
2 |
0.1212 |
Tier I |
| 3 |
NC_000019.10 |
19 |
8335675 |
- |
KANK3 |
NNNGACTTCCTCAAGTACAT |
NGA |
2 |
0.0641 |
Tier I |
| 4 |
NC_000019.10 |
19 |
45088474 |
+ |
GEMIN7 |
NNNGACTTCCACGAGTACAT |
NGT |
2 |
0.0121 |
Tier II |
| 5 |
NC_000023.11 |
X |
9840152 |
- |
SHROOM2 |
NNNGACTTCCTCTAGTACAT |
NTG |
2 |
0.0117 |
Tier II |
| 6 |
NC_000023.11 |
X |
80572845 |
- |
HK2P1 |
NNNGACTTCCTGGAGTACAT |
NGG |
1 |
0.4444 |
Tier III |
| 7 |
NC_000019.10 |
19 |
45088474 |
+ |
GEMIN7-AS1 |
NNNGACTTCCACGAGTACAT |
NGT |
2 |
0.0121 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
82734924 |
- |
Hk2 |
NNNGACTTCCTTGAGTACAT |
NGG |
1 |
0.5385 |
Tier I |
| 2 |
NC_000076.6 |
10 |
62284355 |
- |
Hk1 |
NNNGACTTCCTGGACTACAT |
NGG |
2 |
0.1212 |
Tier I |
| 3 |
NC_000076.6 |
10 |
62398661 |
- |
Hkdc1 |
NNNGACTTCCTGGACTACAT |
NGG |
2 |
0.1212 |
Tier I |
| 4 |
NC_000071.6 |
5 |
77016854 |
- |
Thegl |
NNNGAGTGCCTCGAGTACAT |
NGG |
2 |
0.3667 |
Tier II |
| 5 |
NC_000071.6 |
5 |
77016854 |
- |
1700112J05Rik |
NNNGAGTGCCTCGAGTACAT |
NGG |
2 |
0.3667 |
Tier III |
Other clones with same target sequence:
(none)
