Construct: sgRNA BRDN0001162362
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATCGACTGGGCTCTTACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PANK2 (80025)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76811
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
3889670 |
+ |
PANK2 |
NNNCGACTGGGCTCTTACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
42282364 |
- |
CIC |
NNNAGACTGGGCTCTTACAC |
NGG |
2 |
0.3609 |
Tier II |
| 3 |
NC_000020.11 |
20 |
3805165 |
- |
CDC25B |
NNNAGACTGGGCTCTTCCAG |
NGG |
2 |
0.1486 |
Tier II |
| 4 |
NC_000014.9 |
14 |
95463679 |
- |
SYNE3 |
NNNCGACTTGGCTCTTGCAG |
NGG |
2 |
0.0941 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
140593119 |
+ |
Arhgef10l |
NNNCGTCTGGGCTCTCACAG |
NGG |
2 |
0.4762 |
Tier I |
| 2 |
NC_000071.6 |
5 |
142892495 |
+ |
Fbxl18 |
NNNCAACTGAGCTCTTACAG |
NGG |
2 |
0.8089 |
Tier II |
| 3 |
NC_000083.6 |
17 |
71803760 |
+ |
Clip4 |
NNNTGACTGAGCTCTTACAG |
NGG |
2 |
0.7467 |
Tier II |
| 4 |
NC_000073.6 |
7 |
130464618 |
+ |
Ate1 |
NNNTGACTGTGCTCTTACAG |
NGG |
2 |
0.65 |
Tier II |
| 5 |
NC_000067.6 |
1 |
106655338 |
- |
Bcl2 |
NNNTGACAGGGCTCTTACAG |
NGG |
2 |
0.64 |
Tier II |
| 6 |
NC_000073.6 |
7 |
79744949 |
- |
Wdr93 |
NNNCCACTGGGCTCTTAAAG |
NGG |
2 |
0.4231 |
Tier II |
| 7 |
NC_000083.6 |
17 |
11744968 |
+ |
Prkn |
NNNCGATTGGGCTCTGACAG |
NGG |
2 |
0.2813 |
Tier II |
| 8 |
NC_000071.6 |
5 |
128933752 |
+ |
Rimbp2 |
NNNGTACTGGGCTCTTACAG |
NGG |
2 |
0.15 |
Tier II |
| 9 |
NC_000074.6 |
8 |
22694378 |
+ |
Ikbkb |
NNNCGACAGGGCTCTTCCAG |
NGG |
2 |
0.1412 |
Tier II |
| 10 |
NC_000077.6 |
11 |
107590056 |
- |
Helz |
NNNCCACTGGGCTCTTCCAG |
NGG |
2 |
0.1387 |
Tier II |
| 11 |
NC_000072.6 |
6 |
114458944 |
- |
Hrh1 |
NNNCTACTGGGGTCTTACAG |
NGG |
2 |
0.1333 |
Tier II |
| 12 |
NC_000073.6 |
7 |
46781952 |
- |
Hps5 |
NNNCGACTGGGCTCTTTCAA |
NGG |
2 |
0.125 |
Tier II |
| 13 |
NC_000067.6 |
1 |
190187506 |
+ |
Prox1os |
NNNCGTCTGGGCTCTTAGAG |
NGG |
2 |
0.0952 |
Tier III |
Other clones with same target sequence:
(none)
