Construct: sgRNA BRDN0001162363
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGGGTACCATGCCAGACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TBRG4 (9238)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77704
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
45105755 |
+ |
TBRG4 |
NNNGGTACCATGCCAGACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
42906207 |
+ |
HOOK3 |
NNNTGCACCATGCCAGACCG |
NGG |
2 |
0.3306 |
Tier I |
| 3 |
NC_000007.14 |
7 |
158444966 |
+ |
PTPRN2 |
NNNGGCACCATGCCAGACCA |
NGG |
2 |
0.8523 |
Tier II |
| 4 |
NC_000001.11 |
1 |
44639119 |
+ |
RNF220 |
NNNGGTACCATGCCAAACCT |
NGG |
2 |
0.7 |
Tier II |
| 5 |
NC_000022.11 |
22 |
25110425 |
+ |
KIAA1671 |
NNNGGTACCATGTCAGACCT |
NGG |
2 |
0.49 |
Tier II |
| 6 |
NC_000011.10 |
11 |
2423902 |
- |
TRPM5 |
NNNGGGACCAAGCCAGACCG |
NGG |
2 |
0.4286 |
Tier II |
| 7 |
NC_000005.10 |
5 |
94901501 |
- |
MCTP1 |
NNNGGTACCATCCCAGACAG |
NGG |
2 |
0.2269 |
Tier II |
| 8 |
NC_000005.10 |
5 |
435968 |
- |
AHRR |
NNNGGTTCCAGGCCAGACCG |
NGG |
2 |
0.175 |
Tier II |
| 9 |
NC_000003.12 |
3 |
12149233 |
+ |
SYN2 |
NNNGGTGCCATGCCAGAGCG |
NGG |
2 |
0.0941 |
Tier II |
| 10 |
NC_000001.11 |
1 |
59433564 |
- |
FGGY |
NNNGGTACCATGCCAGACAG |
NTG |
2 |
0.0167 |
Tier II |
| 11 |
NC_000017.11 |
17 |
4457941 |
- |
SPNS3 |
NNNGGTACCATGCCAGACCC |
NGC |
2 |
0.0095 |
Tier II |
| 12 |
NC_000001.11 |
1 |
44639119 |
+ |
LOC107984950 |
NNNGGTACCATGCCAAACCT |
NGG |
2 |
0.7 |
Tier III |
| 13 |
NC_000022.11 |
22 |
25110425 |
+ |
KIAA1671-AS1 |
NNNGGTACCATGTCAGACCT |
NGG |
2 |
0.49 |
Tier III |
| 14 |
NC_000001.11 |
1 |
94744492 |
+ |
SLC44A3-AS1 |
NNNGGTACCATGCCAGACCC |
NGG |
1 |
0.4286 |
Tier III |
| 15 |
NC_000013.11 |
13 |
32858037 |
- |
LINC00423 |
NNNGGTACCATCCCAGACCT |
NGG |
2 |
0.3706 |
Tier III |
| 16 |
NC_000005.10 |
5 |
435968 |
- |
PDCD6-AHRR |
NNNGGTTCCAGGCCAGACCG |
NGG |
2 |
0.175 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
116422285 |
+ |
Hspb8 |
NNNGGTACCATGCCAGACCT |
NAG |
2 |
0.1815 |
Tier I |
| 2 |
NC_000075.6 |
9 |
68745153 |
- |
Rora |
NNNTGTACCATGCCAGACCA |
NGG |
2 |
0.3409 |
Tier II |
| 3 |
NC_000075.6 |
9 |
103272872 |
+ |
1300017J02Rik |
NNNGGTACCATGCCTGACCC |
NGG |
2 |
0.0857 |
Tier II |
| 4 |
NC_000070.6 |
4 |
11371916 |
+ |
Esrp1 |
NNNGGTACCATGCCAGGCAG |
NGG |
2 |
0.0756 |
Tier II |
| 5 |
NC_000067.6 |
1 |
136097597 |
+ |
Cacna1s |
NNNGGTACCATGCCAGTCTG |
NGG |
2 |
0.0615 |
Tier II |
| 6 |
NC_000078.6 |
12 |
110211546 |
- |
Gm34895 |
NNNGGTAACATGCCAGACTG |
NGG |
2 |
0.3 |
Tier III |
Other clones with same target sequence:
(none)
