Construct: sgRNA BRDN0001162367
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GACGGGCCGAGTCATGCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME1-NME2 (654364)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75888
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
51161203 |
+ |
NME1 |
NNNGGGCCGAGTCATGCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
51161203 |
+ |
NME1-NME2 |
NNNGGGCCGAGTCATGCTCG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000014.9 |
14 |
74579983 |
+ |
LTBP2 |
NNNGGGCTGAGTCATGCTCG |
NGG |
1 |
0.875 |
Tier II |
| 4 |
NC_000009.12 |
9 |
77947388 |
- |
GNAQ |
NNNGGGCAGAGTCATGCTTG |
NGG |
2 |
0.3 |
Tier II |
| 5 |
NC_000002.12 |
2 |
46616559 |
- |
PIGF |
NNNGGGCCGGGTCATGCCCG |
NGG |
2 |
0.2381 |
Tier II |
| 6 |
NC_000009.12 |
9 |
137453018 |
+ |
NSMF |
NNNGGGCGGAGTCCTGCTCG |
NGG |
2 |
0.1378 |
Tier II |
| 7 |
NC_000018.10 |
18 |
79022785 |
+ |
LOC105372225 |
NNNGGGCCGAGTCCTGCCCG |
NGG |
2 |
0.0918 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
24601248 |
+ |
Slc39a6 |
NNNGGTCCGAGTGATGCTCG |
NGG |
2 |
0.0909 |
Tier I |
| 2 |
NC_000084.6 |
18 |
24601266 |
+ |
Slc39a6 |
NNNGGTCCGAGTGATGCTCG |
NGG |
2 |
0.0909 |
Tier I |
| 3 |
NC_000084.6 |
18 |
24601284 |
+ |
Slc39a6 |
NNNGGTCCGAGTGATGCTCG |
NGG |
2 |
0.0909 |
Tier I |
| 4 |
NC_000084.6 |
18 |
24601320 |
+ |
Slc39a6 |
NNNGGTCCGAGTGATGCTCG |
NGG |
2 |
0.0909 |
Tier I |
| 5 |
NC_000077.6 |
11 |
93951970 |
- |
Nme2 |
NNNGGGCCGAGTGATGCTGG |
NGG |
2 |
0.017 |
Tier I |
| 6 |
NC_000075.6 |
9 |
107262979 |
+ |
Mapkapk3 |
NNNGAGCAGAGTCATGCTCG |
NGG |
2 |
0.5633 |
Tier II |
| 7 |
NC_000073.6 |
7 |
45918027 |
+ |
Emp3 |
NNNGAGCCGAGTCAGGCTCG |
NGG |
2 |
0.0433 |
Tier II |
| 8 |
NC_000074.6 |
8 |
70010732 |
- |
Gm7730 |
NNNGGGCCGAGTGATGCTGG |
NGG |
2 |
0.017 |
Tier III |
Other clones with same target sequence:
(none)
