Construct: sgRNA BRDN0001162371
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTAACGGCAATGACGTGGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K7 (5609)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77823
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
7910357 |
- |
MAP2K7 |
NNNAACGGCAATGACGTGGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
80160231 |
- |
PARD6G |
NNNAACGGCATTGAGGTGGC |
NGG |
2 |
0.0154 |
Tier I |
| 3 |
NC_000008.11 |
8 |
133248253 |
+ |
NDRG1 |
NNNAAAGGCAATGACGTGCC |
NGG |
2 |
0.4163 |
Tier II |
| 4 |
NC_000023.11 |
X |
14671265 |
+ |
GLRA2 |
NNNAATGGCAATGATGTGGC |
NGG |
2 |
0.0619 |
Tier II |
| 5 |
NC_000007.14 |
7 |
156620714 |
- |
RNF32-DT |
NNNGACGGAAATGACGTGGC |
NGG |
2 |
0.5357 |
Tier III |
| 6 |
NC_000011.10 |
11 |
42248686 |
- |
LINC02740 |
NNNAATGGCAATGATGTGGC |
NGG |
2 |
0.0619 |
Tier III |
| 7 |
NC_000018.10 |
18 |
80160231 |
- |
PARD6G-AS1 |
NNNAACGGCATTGAGGTGGC |
NGG |
2 |
0.0154 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
105702990 |
+ |
Pard6a |
NNNAACGGCATTGAGGTGGC |
NGG |
2 |
0.0154 |
Tier I |
| 2 |
NC_000072.6 |
6 |
55382098 |
- |
Ghrhr |
NNNAAAGGCAATGACATGGC |
NGG |
2 |
0.9286 |
Tier II |
Other clones with same target sequence:
(none)
