Construct: sgRNA BRDN0001162403
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATTGCGATCAAACCAGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME1-NME2 (654364)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75887
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
51155695 |
+ |
NME1 |
NNNTGCGATCAAACCAGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
51155695 |
+ |
NME1-NME2 |
NNNTGCGATCAAACCAGATG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000003.12 |
3 |
123665949 |
+ |
MYLK |
NNNTGCTATCAAACCAGATG |
NGG |
1 |
0.5714 |
Tier II |
| 4 |
NC_000018.10 |
18 |
50968757 |
- |
ELAC1 |
NNNTGCGAGCAAACCAGATT |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000010.11 |
10 |
96976084 |
+ |
LCOR |
NNNTGCAATCAAACCAGGTG |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000011.10 |
11 |
17193572 |
+ |
PIK3C2A |
NNNTGCGATCAAGCCAGGTG |
NGG |
2 |
0.2609 |
Tier II |
| 7 |
NC_000018.10 |
18 |
49262506 |
- |
DYM |
NNNTGCGAACAAACCAGATG |
NGT |
2 |
0.015 |
Tier II |
| 8 |
NC_000008.11 |
8 |
27254112 |
- |
STMN4 |
NNNTGCCATCAAACCAGATG |
NGT |
2 |
0.0111 |
Tier II |
| 9 |
NC_000001.11 |
1 |
68162535 |
+ |
WLS |
NNNTGCGATCAAAGCCGATG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000011.10 |
11 |
17193572 |
+ |
RPL29P21 |
NNNTGCGATCAAGCCAGGTG |
NGG |
2 |
0.2609 |
Tier III |
| 11 |
NC_000004.12 |
4 |
148551208 |
+ |
LOC107986195 |
NNNTGGGATCAAACAAGATG |
NGG |
2 |
0.1111 |
Tier III |
| 12 |
NC_000014.9 |
14 |
55792986 |
- |
LINC00520 |
NNNTGCAATCAAACTAGATG |
NGG |
2 |
0.0667 |
Tier III |
| 13 |
NC_000001.11 |
1 |
68162535 |
+ |
GNG12-AS1 |
NNNTGCGATCAAAGCCGATG |
NGG |
2 |
0.0 |
Tier III |
| 14 |
NC_000001.11 |
1 |
68162535 |
+ |
CTBP2P8 |
NNNTGCGATCAAAGCCGATG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
26533353 |
+ |
Slmap |
NNNTGTGATCAAACCAGACG |
NGG |
2 |
0.2562 |
Tier I |
| 2 |
NC_000071.6 |
5 |
44668878 |
+ |
Ldb2 |
NNNTGCGTTCAAACCAGCTG |
NGG |
2 |
0.0816 |
Tier II |
Other clones with same target sequence:
(none)
