Construct: sgRNA BRDN0001162413
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCTGCACAGACCGAATATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCI (5584)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76402
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
170270453 |
+ |
PRKCI |
NNNTGCACAGACCGAATATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
24502572 |
- |
KIAA1217 |
NNNTGCACAGAGCAAATATG |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000001.11 |
1 |
201617878 |
+ |
NAV1 |
NNNTGCTCAGACAGAATATG |
NGG |
2 |
0.1683 |
Tier II |
| 4 |
NC_000011.10 |
11 |
117665539 |
- |
DSCAML1 |
NNNTGCACAGACCCAATCTG |
NGG |
2 |
0.0816 |
Tier II |
| 5 |
NC_000002.12 |
2 |
12739151 |
+ |
TRIB2 |
NNNTGCACAGACAGAAGATG |
NGG |
2 |
0.0452 |
Tier II |
| 6 |
NC_000023.11 |
X |
101538798 |
- |
PRKCIP1 |
NNNTGCACAGACTGAATATG |
NGG |
1 |
0.7 |
Tier III |
| 7 |
NC_000013.11 |
13 |
57216508 |
- |
LOC105370216 |
NNNTGCATAGACAGAATATG |
NGG |
2 |
0.3365 |
Tier III |
| 8 |
NC_000001.11 |
1 |
201617878 |
+ |
LOC124904482 |
NNNTGCTCAGACAGAATATG |
NGG |
2 |
0.1683 |
Tier III |
| 9 |
NC_000011.10 |
11 |
117665539 |
- |
LOC105369515 |
NNNTGCACAGACCCAATCTG |
NGG |
2 |
0.0816 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
31031116 |
+ |
Prkci |
NNNTGCACAGACAGAATCTG |
NGG |
2 |
0.0733 |
Tier I |
| 2 |
NC_000067.6 |
1 |
194625774 |
+ |
Plxna2 |
NNNGGCACAGACTGAATATG |
NGG |
2 |
0.4375 |
Tier II |
| 3 |
NC_000068.7 |
2 |
41329211 |
+ |
Lrp1b |
NNNTGCACAGACAGAAAATG |
NGG |
2 |
0.2051 |
Tier II |
| 4 |
NC_000077.6 |
11 |
100566778 |
+ |
Ttc25 |
NNNTGCACAGACCAAAGATG |
NGG |
2 |
0.0882 |
Tier II |
| 5 |
NC_000081.6 |
15 |
89552225 |
- |
Shank3 |
NNNTGCACAGACCAAAGATG |
NGG |
2 |
0.0882 |
Tier II |
| 6 |
NC_000069.6 |
3 |
148915291 |
+ |
Adgrl2 |
NNNTGCACAGAACGAAGATG |
NGG |
2 |
0.084 |
Tier II |
| 7 |
NC_000074.6 |
8 |
118264175 |
+ |
Cdh13 |
NNNTGCACAGACTGAATATG |
NGA |
2 |
0.0486 |
Tier II |
| 8 |
NC_000078.6 |
12 |
60216491 |
- |
Gm40431 |
NNNTGCACAGTCCAAATATG |
NGG |
2 |
0.2308 |
Tier III |
| 9 |
NC_000068.7 |
2 |
41329211 |
+ |
Gm30138 |
NNNTGCACAGACAGAAAATG |
NGG |
2 |
0.2051 |
Tier III |
Other clones with same target sequence:
(none)
