Construct: sgRNA BRDN0001162416

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGCCAACCTGTGTACCCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WNK2 (65268)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75671

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 93259286 + WNK2 NNNCCAACCTGTGTACCCAG NGG 0 1.0 Tier I
2 NC_000009.12 9 35563175 + CIMIP2B NNNCAAACCTGTGTACCCAG NGA 2 0.0397 Tier I
3 NC_000016.10 16 31462478 + ARMC5 NNNCCAACCTGTGTGCCCAG NGC 2 0.0144 Tier I
4 NC_000003.12 3 76677181 + ROBO2 NNNCCAAACAGTGTACCCAG NGG 2 0.5571 Tier II
5 NC_000009.12 9 93580995 + PHF2 NNNCCTGCCTGTGTACCCAG NGG 2 0.5042 Tier II
6 NC_000017.11 17 43880576 + MPP2 NNNCCAACCCGTGTACCCAA NGG 2 0.5 Tier II
7 NC_000010.11 10 124608298 + LHPP NNNCCAGCCTGTGTGCCCAG NGG 2 0.4588 Tier II
8 NC_000014.9 14 105397216 - PACS2 NNNCCATCCTGTGTAACCAG NGG 2 0.4375 Tier II
9 NC_000005.10 5 147690892 - JAKMIP2 NNNCCATCCTGTGTACCCAA NGG 2 0.4102 Tier II
10 NC_000003.12 3 46874859 + MYL3 NNNCCAACCAGTGTCCCCAG NGG 2 0.2338 Tier II
11 NC_000009.12 9 130892992 + QRFP NNNCCATCCTCTGTACCCAG NGG 2 0.1875 Tier II
12 NC_000001.11 1 234173547 - SLC35F3 NNNCCAACCTGTGACCCCAG NGG 2 0.1688 Tier II
13 NC_000004.12 4 119602566 + PDE5A NNNCCAAACTGTGTTCCCAG NGG 2 0.13 Tier II
14 NC_000001.11 1 111200624 + DENND2D NNNCCATCCTGTGCACCCAG NGG 2 0.125 Tier II
15 NC_000007.14 7 1852171 - MAD1L1 NNNCCAACCTGTGTCCCCAC NGG 2 0.1169 Tier II
16 NC_000021.9 21 41761274 - RIPK4 NNNCCAACCGGTGTTCCCAG NGG 2 0.1 Tier II
17 NC_000001.11 1 157830729 - CD5L NNNCCAACCTGTGTACCCAA NGA 2 0.0651 Tier II
18 NC_000005.10 5 132006512 + ACSL6 NNNCCAATCTGTGGACCCAG NGG 2 0.0 Tier II
19 NC_000003.12 3 59606650 - CFAP20DC-DT NNNCCATCCTTTGTACCCAG NGG 2 0.1683 Tier III
20 NC_000015.10 15 64718876 + LOC124903507 NNNCCAACCTGTGTTCCCAC NGG 2 0.0857 Tier III
21 NC_000005.10 5 132006512 + ACSL6-AS1 NNNCCAATCTGTGGACCCAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 49076313 - Wnk2 NNNCCAGCCTGTGTACCCAG NAG 2 0.183 Tier I
2 NC_000068.7 2 4896738 - Sephs1 NNNGCAACCTGTGTCCCCAG NGG 2 0.1364 Tier I
3 NC_000070.6 4 58069959 - Svep1 NNNCCAACCTGTGTACCCAT NGA 2 0.0486 Tier I
4 NC_000077.6 11 99949422 + Krtap9-5 NNNCCAACCTGTGTAACCAG NTG 2 0.039 Tier I
5 NC_000074.6 8 88664999 + Nod2 NNNCCAACCTGTGTATCCAG NGC 2 0.0068 Tier I
6 NC_000067.6 1 39923290 - Map4k4 NNNCCTACCTGTGTACCCAA NGG 2 0.6696 Tier II
7 NC_000070.6 4 95847000 - Fggy NNNACAGCCTGTGTACCCAG NGG 2 0.5944 Tier II
8 NC_000082.6 16 10619312 + Clec16a NNNCCAACTTGTGTACTCAG NGG 2 0.4083 Tier II
9 NC_000071.6 5 37393358 - Evc2 NNNCCAACCTGTGTGCCCTG NGG 2 0.35 Tier II
10 NC_000070.6 4 133851546 + Rps6ka1 NNNCCACCGTGTGTACCCAG NGG 2 0.2708 Tier II
11 NC_000077.6 11 120954603 + Slc16a3 NNNCCAACCTCTCTACCCAG NGG 2 0.1805 Tier II
12 NC_000079.6 13 20216794 - Elmo1 NNNCCTACCTGTGTTCCCAG NGG 2 0.1429 Tier II
13 NC_000083.6 17 10455167 - Pacrg NNNCCCACCTGTGTAGCCAG NGG 2 0.0699 Tier II
14 NC_000074.6 8 106297690 + Smpd3 NNNCCACCCTGTGTACCCAG NGA 2 0.0304 Tier II
15 NC_000069.6 3 106844663 + Gm35507 NNNTCTACCTGTGTACCCAG NGG 2 0.5714 Tier III
16 NC_000068.7 2 34030184 + C230014O12Rik NNNCCCACCTGTGTAACCAG NGG 2 0.4545 Tier III
17 NC_000085.6 19 57342216 + B230217O12Rik NNNCCAACCTCTGTACCCTG NGG 2 0.2308 Tier III
18 NC_000074.6 8 106297690 + 4930506A18Rik NNNCCACCCTGTGTACCCAG NGA 2 0.0304 Tier III
19 NC_000083.6 17 82946783 - Gm34265 NNNCCAACCTGTTTACCCAG NGC 2 0.0067 Tier III
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Other clones with same target sequence:

(none)