Construct: sgRNA BRDN0001162423
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACACCTGTCTTGAACGAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK1 (27102)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77048
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
6054568 |
+ |
EIF2AK1 |
NNNACCTGTCTTGAACGAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
130965136 |
- |
EPB41L2 |
NNNACTTGTCTTGAAAGAAG |
NGG |
2 |
0.9286 |
Tier II |
| 3 |
NC_000022.11 |
22 |
46888261 |
+ |
TBC1D22A |
NNNACCTGTCCTGAAAGAAG |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000003.12 |
3 |
7716079 |
+ |
GRM7 |
NNNACCTGTCTTGAATGCAG |
NGG |
2 |
0.0586 |
Tier II |
| 5 |
NC_000007.14 |
7 |
77007005 |
+ |
DTX2P1-UPK3BP1-PMS2P11 |
NNNCCCTGTCTTGAACGAAC |
NGG |
2 |
0.1513 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
71659344 |
- |
Cgnl1 |
NNNACTTGTCTTGAACAAAG |
NGG |
2 |
0.8667 |
Tier II |
| 2 |
NC_000076.6 |
10 |
3755733 |
+ |
Plekhg1 |
NNNACTTGTCTTGAACAAAG |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000068.7 |
2 |
32649676 |
+ |
Eng |
NNNACCTGTCTTGAGAGAAG |
NGG |
2 |
0.65 |
Tier II |
| 4 |
NC_000085.6 |
19 |
40734688 |
+ |
Entpd1 |
NNNACCTGTATTGTACGAAG |
NGG |
2 |
0.4622 |
Tier II |
| 5 |
NC_000084.6 |
18 |
53891339 |
- |
Csnk1g3 |
NNNCCCTGTCTTGAACAAAG |
NGG |
2 |
0.3294 |
Tier II |
| 6 |
NC_000083.6 |
17 |
27477723 |
- |
Grm4 |
NNNACCTGTTTTGAATGAAG |
NGG |
2 |
0.2896 |
Tier II |
| 7 |
NC_000071.6 |
5 |
89437604 |
- |
Gc |
NNNACCTGTCTTGAACCAAA |
NGG |
2 |
0.2206 |
Tier II |
| 8 |
NC_000082.6 |
16 |
56005301 |
+ |
Zbtb11 |
NNNAACTGTCTTGAATGAAG |
NGG |
2 |
0.1758 |
Tier II |
| 9 |
NC_000080.6 |
14 |
19899166 |
- |
Gng2 |
NNNACCTGTCTTGAACCAAT |
NGG |
2 |
0.1647 |
Tier II |
| 10 |
NC_000080.6 |
14 |
70616955 |
- |
Dmtn |
NNNACCTGTCTTCAAGGAAG |
NGG |
2 |
0.0648 |
Tier II |
| 11 |
NC_000074.6 |
8 |
83038373 |
- |
Rnf150 |
NNNACCTGTCTTGAACAAAG |
NGA |
2 |
0.0648 |
Tier II |
| 12 |
NC_000076.6 |
10 |
3755733 |
+ |
Gm30164 |
NNNACTTGTCTTGAACAAAG |
NGG |
2 |
0.8667 |
Tier III |
| 13 |
NC_000071.6 |
5 |
23891982 |
- |
Gm9534 |
NNNACCTGTCTTGAAAGAAG |
NGA |
2 |
0.0694 |
Tier III |
Other clones with same target sequence:
(none)
