Construct: sgRNA BRDN0001162447
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCAGCGGGGATATGGTGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80239
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
113113070 |
- |
MCC |
NNNGGCAGGGATATGGTGAA |
NGG |
2 |
0.625 |
Tier II |
| 2 |
NC_000015.10 |
15 |
62765044 |
- |
TLN2 |
NNNAGGGAGGATATGGTGAA |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000010.11 |
10 |
48362216 |
- |
MAPK8 |
NNNATGGGGGATATGGTGAA |
NGG |
2 |
0.15 |
Tier II |
| 4 |
NC_000002.12 |
2 |
219055986 |
- |
IHH |
NNNAGCTGGGATATGGTGAC |
NGG |
2 |
0.1299 |
Tier II |
| 5 |
NC_000023.11 |
X |
102721251 |
+ |
GPRASP3 |
NNNAGCGGGGAAATGGTGAA |
NTG |
2 |
0.0312 |
Tier II |
| 6 |
NC_000023.11 |
X |
102721251 |
+ |
ARMCX5-GPRASP2 |
NNNAGCGGGGAAATGGTGAA |
NTG |
2 |
0.0312 |
Tier II |
| 7 |
NC_000012.12 |
12 |
5325828 |
- |
LOC105369617 |
NNNAGGGGGGATATGGAGAA |
NGG |
2 |
0.2667 |
Tier III |
| 8 |
NC_000012.12 |
12 |
49913755 |
+ |
LINC02395 |
NNNAGGGGGGATCTGGTGAA |
NGG |
2 |
0.1053 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
53468526 |
+ |
Msx2 |
NNNAGCTGGGATGTGGTGAA |
NGG |
2 |
0.3727 |
Tier I |
| 2 |
NC_000072.6 |
6 |
127638702 |
- |
Cracr2a |
NNNAGCGGGGACATGGCGAA |
NGG |
2 |
0.6687 |
Tier II |
| 3 |
NC_000073.6 |
7 |
89708776 |
+ |
Me3 |
NNNTGTGGGGATATGGTGAA |
NGG |
2 |
0.5909 |
Tier II |
| 4 |
NC_000074.6 |
8 |
32089090 |
+ |
Nrg1 |
NNNAGAGGGGATATGGTGAA |
NAG |
2 |
0.2407 |
Tier II |
| 5 |
NC_000069.6 |
3 |
26659678 |
+ |
Spata16 |
NNNAGCGGGGAAATGGTGAA |
NAG |
2 |
0.2074 |
Tier II |
| 6 |
NC_000083.6 |
17 |
73904540 |
- |
Xdh |
NNNAGCAGGGATATGGTGAA |
NGC |
2 |
0.0222 |
Tier II |
| 7 |
NC_000073.6 |
7 |
96544160 |
- |
Tenm4 |
NNNAGCTGGGATAGGGTGAA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000079.6 |
13 |
53468526 |
+ |
Gm33763 |
NNNAGCTGGGATGTGGTGAA |
NGG |
2 |
0.3727 |
Tier III |
| 9 |
NC_000083.6 |
17 |
19489470 |
+ |
4930549P19Rik |
NNNAGCAGGGATAGGGTGAA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
