Construct: sgRNA BRDN0001162457
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GACACTCACATCATAGGCGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMK2A (815)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76930
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
150251758 |
+ |
CAMK2A |
NNNACTCACATCATAGGCGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
14200326 |
- |
TRIO |
NNNACTTACATCATGGGCGC |
NGG |
2 |
0.5281 |
Tier II |
| 3 |
NC_000006.12 |
6 |
36905317 |
- |
C6orf89 |
NNNACTCACATCAGAGGCGA |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000010.11 |
10 |
64008020 |
- |
LOC124902439 |
NNNATTCACATCATAGGCAC |
NGG |
2 |
0.4545 |
Tier III |
| 5 |
NC_000012.12 |
12 |
89989857 |
- |
LOC105369890 |
NNNACTCACAACATAGGAGC |
NGG |
2 |
0.4038 |
Tier III |
| 6 |
NC_000004.12 |
4 |
19405466 |
- |
LINC02438 |
NNNGCTCACATCATAGGCGA |
NGG |
2 |
0.3125 |
Tier III |
| 7 |
NC_000006.12 |
6 |
131963793 |
- |
CCN2-AS1 |
NNNACTCACATCATAGGGGT |
NGG |
2 |
0.04 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
31042958 |
+ |
Slc5a6 |
NNNGCTCACCTCATAGGCGC |
NGG |
2 |
0.2083 |
Tier I |
| 2 |
NC_000084.6 |
18 |
60957387 |
- |
Camk2a |
NNNACTCACATCATAGGCAC |
NAG |
2 |
0.1852 |
Tier I |
| 3 |
NC_000075.6 |
9 |
102218366 |
+ |
Ephb1 |
NNNACTCACATCATAGACAC |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000080.6 |
14 |
121199984 |
- |
Farp1 |
NNNACACACAGCATAGGCGC |
NGG |
2 |
0.3467 |
Tier II |
| 5 |
NC_000069.6 |
3 |
79510479 |
- |
Fnip2 |
NNNTCTCACATCATAGGCCC |
NGG |
2 |
0.2853 |
Tier II |
| 6 |
NC_000068.7 |
2 |
22628393 |
+ |
Gad2 |
NNNACTCACATCACAGGCAC |
NGG |
2 |
0.2041 |
Tier II |
| 7 |
NC_000067.6 |
1 |
40616972 |
- |
Slc9a4 |
NNNACTCACATCATAGGTGC |
NAG |
2 |
0.1667 |
Tier II |
| 8 |
NC_000075.6 |
9 |
102218366 |
+ |
Gm31233 |
NNNACTCACATCATAGACAC |
NGG |
2 |
0.6667 |
Tier III |
| 9 |
NC_000069.6 |
3 |
79510479 |
- |
Gm36442 |
NNNTCTCACATCATAGGCCC |
NGG |
2 |
0.2853 |
Tier III |
| 10 |
NC_000068.7 |
2 |
95460632 |
+ |
Gm13794 |
NNNGCTCACATCATAGGGGC |
NGG |
2 |
0.0833 |
Tier III |
Other clones with same target sequence:
(none)
