Construct: sgRNA BRDN0001162486
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCAACACCATTCAGTACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAOK2 (9344)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77250
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
29978320 |
+ |
TAOK2 |
NNNAACACCATTCAGTACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
55105029 |
+ |
USP24 |
NNNAACACCATTCAGTACAA |
NGG |
2 |
0.4018 |
Tier II |
| 3 |
NC_000007.14 |
7 |
99690375 |
+ |
ZSCAN25 |
NNNAATACCATTCAGTACAG |
NGG |
2 |
0.398 |
Tier II |
| 4 |
NC_000007.14 |
7 |
99690375 |
+ |
CYP3A7-CYP3A51P |
NNNAATACCATTCAGTACAG |
NGG |
2 |
0.398 |
Tier II |
| 5 |
NC_000012.12 |
12 |
56184098 |
- |
SMARCC2 |
NNNATCACCATTCAGTACCT |
NGG |
2 |
0.2545 |
Tier II |
| 6 |
NC_000006.12 |
6 |
136192068 |
+ |
PDE7B |
NNNAACTCCATTCAGTAACG |
NGG |
2 |
0.2356 |
Tier II |
| 7 |
NC_000004.12 |
4 |
121881076 |
+ |
TRPC3 |
NNNCACACCATTCAGTACCG |
NAG |
2 |
0.0915 |
Tier II |
| 8 |
NC_000007.14 |
7 |
99690375 |
+ |
CYP3A51P |
NNNAATACCATTCAGTACAG |
NGG |
2 |
0.398 |
Tier III |
| 9 |
NC_000013.11 |
13 |
66281724 |
- |
LOC124903179 |
NNNAACACCATTCAGGACCT |
NGG |
2 |
0.2423 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
126880086 |
- |
Taok2 |
NNNAATACCATTCAGTACCG |
NGG |
1 |
0.9286 |
Tier I |
| 2 |
NC_000068.7 |
2 |
43638889 |
- |
Kynu |
NNNAACACCATTGAGTACCA |
NGG |
2 |
0.1278 |
Tier II |
| 3 |
NC_000085.6 |
19 |
53774388 |
- |
Rbm20 |
NNNAACTCCATTCAGTCCCG |
NGG |
2 |
0.0772 |
Tier II |
| 4 |
NC_000086.7 |
X |
139640368 |
- |
Rnf128 |
NNNAACACCATTGAGTACCC |
NGG |
2 |
0.0584 |
Tier II |
| 5 |
NC_000084.6 |
18 |
60586763 |
- |
Myoz3 |
NNNAACACCATTGAGTACGG |
NGG |
2 |
0.017 |
Tier II |
| 6 |
NC_000069.6 |
3 |
95171956 |
- |
Sema6c |
NNNAACACCAATCAGTACCG |
NGC |
2 |
0.0167 |
Tier II |
| 7 |
NC_000071.6 |
5 |
23386957 |
- |
5031425E22Rik |
NNNAACACCAGTCAGTACCG |
NGG |
1 |
0.4 |
Tier III |
| 8 |
NC_000085.6 |
19 |
53774388 |
- |
Gm30890 |
NNNAACTCCATTCAGTCCCG |
NGG |
2 |
0.0772 |
Tier III |
Other clones with same target sequence:
(none)
