Construct: sgRNA BRDN0001162492
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAACACTACCAGAGAACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKFB1 (5207)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77632
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
54958896 |
- |
PFKFB1 |
NNNACACTACCAGAGAACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
66602370 |
+ |
MAST4 |
NNNTCACTACCAAAGAACGA |
NGG |
2 |
0.5874 |
Tier II |
| 3 |
NC_000002.12 |
2 |
175928289 |
- |
LNPK |
NNNACACTACCTGAAAACGA |
NGG |
2 |
0.3137 |
Tier II |
| 4 |
NC_000023.11 |
X |
152941209 |
- |
ZNF185 |
NNNCCACTACCTGAGAACGA |
NGG |
2 |
0.1176 |
Tier II |
| 5 |
NC_000003.12 |
3 |
25607541 |
- |
TOP2B |
NNNACACTACCAGATAACTA |
NGG |
2 |
0.0952 |
Tier II |
| 6 |
NC_000018.10 |
18 |
49180329 |
- |
DYM |
NNNTCACTACCAGAGAACGA |
NGA |
2 |
0.0442 |
Tier II |
| 7 |
NC_000011.10 |
11 |
6943024 |
- |
ZNF215 |
NNNACACTACCAGAGAAGGA |
NTG |
2 |
0.0052 |
Tier II |
| 8 |
NC_000015.10 |
15 |
95474938 |
- |
LINC00924 |
NNNACACAACCAGAGAACAA |
NGG |
2 |
0.5714 |
Tier III |
| 9 |
NC_000015.10 |
15 |
95474938 |
- |
LOC105370993 |
NNNACACAACCAGAGAACAA |
NGG |
2 |
0.5714 |
Tier III |
| 10 |
NC_000002.12 |
2 |
120552996 |
- |
LOC105373585 |
NNNACGCTACCAGAGGACGA |
NGG |
2 |
0.1374 |
Tier III |
| 11 |
NC_000023.11 |
X |
152941209 |
- |
LOC105373372 |
NNNCCACTACCTGAGAACGA |
NGG |
2 |
0.1176 |
Tier III |
| 12 |
NC_000007.14 |
7 |
149799242 |
- |
SSPOP |
NNNACACTACCAGAGACCCA |
NGG |
2 |
0.0791 |
Tier III |
| 13 |
NC_000011.10 |
11 |
6943024 |
- |
LOC107984019 |
NNNACACTACCAGAGAAGGA |
NTG |
2 |
0.0052 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
150613907 |
+ |
Pfkfb1 |
NNNACACTACCAGAGAACGA |
NGA |
1 |
0.0694 |
Tier I |
| 2 |
NC_000079.6 |
13 |
63562628 |
+ |
Ptch1 |
NNNACACTAACAAAGAACGA |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000082.6 |
16 |
49134702 |
+ |
Myh15 |
NNNACACTACAAGAGAACTA |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000067.6 |
1 |
62517614 |
+ |
Pard3b |
NNNACACTACCAAAGAAAGA |
NGG |
2 |
0.497 |
Tier II |
| 5 |
NC_000075.6 |
9 |
63685276 |
+ |
Smad3 |
NNNACACTACCAAAGAAAGA |
NGG |
2 |
0.497 |
Tier II |
| 6 |
NC_000075.6 |
9 |
34683747 |
- |
Kirrel3 |
NNNACACAACCAGAGAAAGA |
NGG |
2 |
0.4308 |
Tier II |
| 7 |
NC_000084.6 |
18 |
38691456 |
- |
Arhgap26 |
NNNACTCTACCAGAGAAAGA |
NGG |
2 |
0.3846 |
Tier II |
| 8 |
NC_000068.7 |
2 |
70062213 |
- |
Myo3b |
NNNACACTACCTGAGAATGA |
NGG |
2 |
0.2143 |
Tier II |
| 9 |
NC_000071.6 |
5 |
8985220 |
+ |
Crot |
NNNACAGTACCACAGAACGA |
NGG |
2 |
0.1981 |
Tier II |
| 10 |
NC_000082.6 |
16 |
72098324 |
+ |
Robo1 |
NNNACACTACCAGAGAATGA |
NAG |
2 |
0.1667 |
Tier II |
| 11 |
NC_000081.6 |
15 |
86175409 |
- |
Cerk |
NNNACACTACCAGAGAACCA |
NTG |
2 |
0.0175 |
Tier II |
| 12 |
NC_000071.6 |
5 |
26922621 |
+ |
Dpp6 |
NNNACACTAGCAGAGAACGA |
NTG |
2 |
0.0152 |
Tier II |
| 13 |
NC_000067.6 |
1 |
62517614 |
+ |
Gm34881 |
NNNACACTACCAAAGAAAGA |
NGG |
2 |
0.497 |
Tier III |
| 14 |
NC_000070.6 |
4 |
120194106 |
+ |
Gm36013 |
NNNACTCTAGCAGAGAACGA |
NGG |
2 |
0.2778 |
Tier III |
| 15 |
NC_000070.6 |
4 |
120194106 |
+ |
Gm36134 |
NNNACTCTAGCAGAGAACGA |
NGG |
2 |
0.2778 |
Tier III |
| 16 |
NC_000074.6 |
8 |
96382223 |
- |
Gm32122 |
NNNACACTACCAGAGAGAGA |
NGG |
2 |
0.095 |
Tier III |
Other clones with same target sequence:
(none)
