Construct: sgRNA BRDN0001162500

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTCTGGCATCCATTGACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK10 (6793)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75490

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 172093718 + STK10 NNNCTGGCATCCATTGACAC NGG 0 1.0 Tier I
2 NC_000006.12 6 84048633 + MRAP2 NNNGTGGCAACCATTGACAC NGG 2 0.4286 Tier II
3 NC_000003.12 3 14700624 + C3orf20 NNNGTTGCATCCATTGACAC NGG 2 0.3333 Tier II
4 NC_000017.11 17 76441198 + UBE2O NNNCAGGAATCCATTGACAC NGG 2 0.325 Tier II
5 NC_000019.10 19 11132245 - LDLR NNNCCGGCATTCATTGACAC NGG 2 0.3077 Tier II
6 NC_000001.11 1 110163200 + SLC6A17 NNNCTGGCTTCCTTTGACAC NGG 2 0.18 Tier II
7 NC_000012.12 12 124465594 - NCOR2 NNNCTGGCTTCCACTGACAC NGG 2 0.1714 Tier II
8 NC_000005.10 5 169052259 - SLIT3 NNNCTGGCATTCATTGACTC NGG 2 0.1657 Tier II
9 NC_000010.11 10 16861094 + CUBN NNNCTGGCATCCATTGAAAT NGG 2 0.1615 Tier II
10 NC_000013.11 13 32384085 - BRCA2 NNNCAGGCATCCATTGACCC NGG 2 0.1034 Tier II
11 NC_000001.11 1 44802628 + PLK3 NNNCTGGCATCCATTGTCCC NGG 2 0.0276 Tier II
12 NC_000013.11 13 70041621 + KLHL1 NNNCTGGCATCCACTGACAC NGT 2 0.0046 Tier II
13 NC_000015.10 15 27964484 - OCA2 NNNCTGCCATCCAGTGACAC NGG 2 0.0 Tier II
14 NC_000006.12 6 161670015 + PRKN NNNCTGGCATCCAGTGACAT NGG 2 0.0 Tier II
15 NC_000011.10 11 134403324 + B3GAT1 NNNCTGGCATCCCTTCACAC NGG 2 0.0 Tier II
16 NC_000001.11 1 39050274 - LOC124904088 NNNTTGGCCTCCATTGACAC NGG 2 0.4571 Tier III
17 NC_000003.12 3 12860338 + LOC124909346 NNNCTGGCATCCACTGGCAC NGG 2 0.0504 Tier III
18 NC_000013.11 13 28543390 - LOC124903142 NNNCTGGCAGCCATTGACAC NGA 2 0.0347 Tier III
19 NC_000013.11 13 53545772 - LOC105370210 NNNCTGGCACCCATGGACAC NGG 2 0.0267 Tier III
20 NC_000010.11 10 10446569 - CELF2-DT NNNCTGGCATCCATTGTCAC NGC 2 0.003 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 110866772 - Zfp839 NNNCTGGCATCCATTGGCAG NGG 2 0.0104 Tier I
2 NC_000077.6 11 32598679 - Stk10 NNNCTGGCATCCATGGACAG NGG 2 0.0029 Tier I
3 NC_000073.6 7 66801882 + Cers3 NNNCTGGCTTCCATTGACAC NGG 1 0.6 Tier II
4 NC_000075.6 9 114495074 - Ccr4 NNNCTGGCCTCCATTAACAC NGG 2 0.5714 Tier II
5 NC_000069.6 3 117837021 - Snx7 NNNCTGACATCCATTGACAA NGG 2 0.5 Tier II
6 NC_000068.7 2 139575313 - Sptlc3 NNNCTTGAATCCATTGACAC NGG 2 0.4333 Tier II
7 NC_000082.6 16 4048896 - Trap1 NNNCTGGAATCCATTGATAC NGG 2 0.4179 Tier II
8 NC_000076.6 10 9522946 - Samd5 NNNTTGGCATCCATTGACAA NGG 2 0.4 Tier II
9 NC_000084.6 18 45596854 + Kcnn2 NNNCTGGCATCTGTTGACAC NGG 2 0.3512 Tier II
10 NC_000067.6 1 32539706 + Khdrbs2 NNNCTGGAATCCATTGAAAC NGG 2 0.35 Tier II
11 NC_000081.6 15 99515131 + Faim2 NNNCTGGCCTCCATAGACAC NGG 2 0.3308 Tier II
12 NC_000083.6 17 48514373 - Unc5cl NNNCTGGCATCCACTGACAC NGG 1 0.2857 Tier II
13 NC_000082.6 16 41760685 + Lsamp NNNCTGGCATCTATTGACAA NGG 2 0.2692 Tier II
14 NC_000081.6 15 94334510 - Adamts20 NNNTTGGCATCCATTGACAC NAG 2 0.2074 Tier II
15 NC_000078.6 12 33191527 + Atxn7l1 NNNCTGGCATCCACAGACAC NGG 2 0.1654 Tier II
16 NC_000068.7 2 155833804 + Fam83c NNNCTGGCCTCCACTGACAC NGG 2 0.1633 Tier II
17 NC_000071.6 5 150787071 + Pds5b NNNCTGGCCTCCATTGACAC NAG 2 0.1481 Tier II
18 NC_000074.6 8 122625949 - Cbfa2t3 NNNCTGGCCTCCCTTGACAC NGG 2 0.1203 Tier II
19 NC_000067.6 1 110071779 + Cdh7 NNNCTGGCATTCACTGACAC NGG 2 0.0879 Tier II
20 NC_000077.6 11 113911844 + Sdk2 NNNCTGGCATCCATTGAGTC NGG 2 0.0718 Tier II
21 NC_000069.6 3 84929364 + Fbxw7 NNNCTGGCAGCCATTGAGAC NGG 2 0.0667 Tier II
22 NC_000086.7 X 168454003 - Frmpd4 NNNCTGGCATTCCTTGACAC NGG 2 0.0648 Tier II
23 NC_000086.7 X 83082913 + Dmd NNNCTGGCATCCATGGACAC NGG 1 0.05 Tier II
24 NC_000067.6 1 37232052 + Cnga3 NNNCTGGCATCCATAGACAG NGG 2 0.0341 Tier II
25 NC_000074.6 8 12984799 - Mcf2l NNNCTGGCTTCCATGGACAC NGG 2 0.03 Tier II
26 NC_000070.6 4 123537798 - Macf1 NNNCTGGCCTCCATGGACAC NGG 2 0.0286 Tier II
27 NC_000071.6 5 88959721 - Slc4a4 NNNCTGGCATCCATTGTCCC NGG 2 0.0276 Tier II
28 NC_000076.6 10 64827025 - Ctnna3 NNNCTGGCCTCCATTGACAC NTG 2 0.0223 Tier II
29 NC_000082.6 16 24760605 - Lpp NNNCTGGCATCCATGGACAC NAG 2 0.013 Tier II
30 NC_000071.6 5 65936026 - Chrna9 NNNCTGGCATCCATGGGCAC NGG 2 0.0088 Tier II
31 NC_000072.6 6 70866674 + Eif2ak3 NNNCTGGCATCCATGGGCAC NGG 2 0.0088 Tier II
32 NC_000071.6 5 130595304 - Caln1 NNNCTGGCATCCATTGGCAC NTG 2 0.0069 Tier II
33 NC_000067.6 1 127413740 + Mgat5 NNNCTGGCATCCATTGACAG NGA 2 0.0041 Tier II
34 NC_000072.6 6 28654476 - Snd1 NNNCTGGCATCCATGGACAC NGA 2 0.0035 Tier II
35 NC_000076.6 10 123041763 + Mon2 NNNCTGGCATCCATGGACAC NGA 2 0.0035 Tier II
36 NC_000070.6 4 121014165 + Smap2 NNNCTGGCATCCATGGACAC NGC 2 0.0011 Tier II
37 NC_000067.6 1 54840848 + Ankrd44 NNNCTGGCATCCATGGACAC NGT 2 0.0008 Tier II
38 NC_000070.6 4 103077749 + Wdr78 NNNCTGGCATCCATTTATAC NGG 2 0.0 Tier II
39 NC_000070.6 4 118898839 - Gm12865 NNNCTGCCATCCATTGACAT NGG 2 0.2063 Tier III
40 NC_000079.6 13 81034085 - 9330111N05Rik NNNCTGGCATCCACAGACAC NGG 2 0.1654 Tier III
41 NC_000086.7 X 168454003 - Gm7209 NNNCTGGCATTCCTTGACAC NGG 2 0.0648 Tier III
42 NC_000081.6 15 103096094 + Gm28876 NNNCTGGCTTCCATGGACAC NGG 2 0.03 Tier III
43 NC_000070.6 4 52026471 + C630028M04Rik NNNCTGGCCTCCATTGACAC NTG 2 0.0223 Tier III
44 NC_000067.6 1 127413740 + Gm51746 NNNCTGGCATCCATTGACAG NGA 2 0.0041 Tier III
45 NC_000074.6 8 96360687 - Gm32005 NNNCTGGCATTCATTTACAC NGG 2 0.0 Tier III
46 NC_000070.6 4 52286840 + Gm12467 NNNCTGGCCTCCAGTGACAC NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)