Construct: sgRNA BRDN0001162507
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGATACTCGGGGGTCCCACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMKV (79012)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75528
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
49861199 |
+ |
CAMKV |
NNNTACTCGGGGGTCCCACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
86064148 |
+ |
PSKH2 |
NNNTACTCTGGGGTCCCACA |
NAG |
2 |
0.1383 |
Tier I |
| 3 |
NC_000023.11 |
X |
11490486 |
- |
ARHGAP6 |
NNNTGCTCAGGGGTCCCACA |
NGG |
2 |
0.4629 |
Tier II |
| 4 |
NC_000010.11 |
10 |
79097183 |
+ |
ZMIZ1 |
NNNTGCTGGGGGGTCCCACA |
NGG |
2 |
0.4629 |
Tier II |
| 5 |
NC_000012.12 |
12 |
124310278 |
- |
RFLNA |
NNNTACTCGGGGGTCCTTCA |
NGG |
2 |
0.2333 |
Tier II |
| 6 |
NC_000012.12 |
12 |
124310278 |
- |
ZNF664-RFLNA |
NNNTACTCGGGGGTCCTTCA |
NGG |
2 |
0.2333 |
Tier II |
| 7 |
NC_000003.12 |
3 |
197769913 |
- |
FYTTD1 |
NNNTACTCGGGGGACCCACA |
NGT |
2 |
0.01 |
Tier II |
| 8 |
NC_000004.12 |
4 |
107363 |
+ |
WEE1P1 |
NNNCACCCGGGGGTCCCACA |
NGG |
2 |
0.4449 |
Tier III |
| 9 |
NC_000011.10 |
11 |
82003178 |
- |
MIR4300HG |
NNNGACTCGGGGGCCCCACA |
NGG |
2 |
0.1786 |
Tier III |
| 10 |
NC_000012.12 |
12 |
54991327 |
- |
LOC107984515 |
NNNTACTCTGGGGTGCCACA |
NGG |
2 |
0.0267 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
107946442 |
- |
Camkv |
NNNTACTCCGGGGTTCCACA |
NGG |
2 |
0.0359 |
Tier I |
| 2 |
NC_000075.6 |
9 |
29201624 |
+ |
Ntm |
NNNGACTCTGGGGTCCCACA |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000072.6 |
6 |
121375843 |
- |
Iqsec3 |
NNNTTCTAGGGGGTCCCACA |
NGG |
2 |
0.2364 |
Tier II |
| 4 |
NC_000067.6 |
1 |
38601354 |
- |
Aff3 |
NNNTTCTCGGGGGCCCCACA |
NGG |
2 |
0.1039 |
Tier II |
| 5 |
NC_000083.6 |
17 |
66120000 |
+ |
Washc1 |
NNNCACTCGGGGGTGCCACA |
NGG |
2 |
0.0324 |
Tier II |
| 6 |
NC_000072.6 |
6 |
60145788 |
+ |
Gm5570 |
NNNTACTCAGGGGTCCCAGA |
NGG |
2 |
0.0804 |
Tier III |
Other clones with same target sequence:
(none)
