Construct: sgRNA BRDN0001162514

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGAGGCCATCTCCCATGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMKV (79012)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75527

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 49860476 - CAMKV NNNAGGCCATCTCCCATGAG NGG 0 1.0 Tier I
2 NC_000001.11 1 222628967 - MIA3 NNNTGGTCATCTCCCATGAG NGG 2 0.517 Tier I
3 NC_000009.12 9 135791051 + KCNT1 NNNAGGCTGTCTCCCATGAG NGG 2 0.5833 Tier II
4 NC_000002.12 2 42767973 - HAAO NNNAGACCATCTCCCATGTG NGG 2 0.5385 Tier II
5 NC_000005.10 5 15844536 + FBXL7 NNNAGGCCATCTCTCATGAT NGG 2 0.5133 Tier II
6 NC_000008.11 8 6475361 - MCPH1 NNNAGCACATCTCCCATGAG NGG 2 0.5114 Tier II
7 NC_000005.10 5 172898827 - ERGIC1 NNNTGGCCATCTCCCATTAG NGG 2 0.4242 Tier II
8 NC_000010.11 10 418922 + DIP2C NNNAGGCCACCTTCCATGAG NGG 2 0.3733 Tier II
9 NC_000003.12 3 196308711 + DYNLT2B NNNAGGCAATCTCCCATGTG NGG 2 0.35 Tier II
10 NC_000002.12 2 70728695 - ADD2 NNNAGGGCTTCTCCCATGAG NGG 2 0.2824 Tier II
11 NC_000001.11 1 153172125 + SPRR2G NNNAAGCCATGTCCCATGAG NGG 2 0.2167 Tier II
12 NC_000002.12 2 49633946 - LOC124906005 NNNAGTCCATTTCCCATGAG NGG 2 0.2051 Tier II
13 NC_000004.12 4 76771569 + SHROOM3 NNNAGGGCATCTCACATGAG NGG 2 0.1647 Tier II
14 NC_000020.11 20 62419858 - RBBP8NL NNNAGGCCACCTCCCATGAG NAG 2 0.1383 Tier II
15 NC_000004.12 4 102251847 + SLC39A8 NNNAGGCCATCTCCCATGTG NGA 2 0.0374 Tier II
16 NC_000005.10 5 149030705 - SH3TC2 NNNAGGCCATCTCCCAGGAG NAG 2 0.0305 Tier II
17 NC_000022.11 22 38244890 + TMEM184B NNNAGGCCAGCTCCCATGAG NGC 2 0.0111 Tier II
18 NC_000001.11 1 44890127 - EIF2B3 NNNAGCCCATCTCCCATGAG NGT 2 0.011 Tier II
19 NC_000005.10 5 73697538 + ARHGEF28 NNNAGGCCATCTCACCTGAG NGG 2 0.0 Tier II
20 NC_000005.10 5 5414739 + LOC101929200 NNNAGTCCATCTCCCATGAG NGG 1 0.6667 Tier III
21 NC_000009.12 9 135791051 + LOC107987140 NNNAGGCTGTCTCCCATGAG NGG 2 0.5833 Tier III
22 NC_000008.11 8 6475361 - LOC101928016 NNNAGCACATCTCCCATGAG NGG 2 0.5114 Tier III
23 NC_000009.12 9 2472809 - LOC101930053 NNNAGCCCATCTCCCATGAT NGG 2 0.4773 Tier III
24 NC_000004.12 4 76771569 + SHROOM3-AS1 NNNAGGGCATCTCACATGAG NGG 2 0.1647 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 107947156 + Camkv NNNAGGCCATCTCCCACGAA NGG 2 0.6618 Tier I
2 NC_000068.7 2 26387258 + Entr1 NNNAGGCCATATCCCATGAG NTG 2 0.0292 Tier I
3 NC_000067.6 1 3397000 - Xkr4 NNNACTCCATCTCCCATGAG NGG 2 0.5238 Tier II
4 NC_000075.6 9 44266455 + Nlrx1 NNNGGGCCATCTCCCAAGAG NGG 2 0.3333 Tier II
5 NC_000081.6 15 7137394 + Lifr NNNAGGCCAACTCCCAGGAG NGG 2 0.1008 Tier II
6 NC_000085.6 19 10796539 + Cd6 NNNAGGCCACCTGCCATGAG NGG 2 0.0727 Tier II
7 NC_000080.6 14 33121563 - Wdfy4 NNNAGGCCATCTCCCATGAG NGA 1 0.0694 Tier II
8 NC_000085.6 19 34808674 + Pank1 NNNAGGCCATTTCCAATGAG NGG 2 0.0684 Tier II
9 NC_000067.6 1 127225740 + Mgat5 NNNAGGCCATATCCCATGAG NGA 2 0.0521 Tier II
10 NC_000073.6 7 46260576 + Otog NNNAGGCCATTTCCCATGAG NTG 2 0.012 Tier II
11 NC_000077.6 11 78975263 - Lgals9 NNNAGCCCATCTCCCATGAG NGT 2 0.011 Tier II
12 NC_000067.6 1 156289811 + Tdrd5 NNNAGGCCATCGCCCATGAG NGT 2 0.0081 Tier II
13 NC_000082.6 16 42249846 + Gap43 NNNAGGCAATCTCCCTTGAG NGG 2 0.0 Tier II
14 NC_000074.6 8 18800147 - Mcph1 NNNAGGCCATCTCCCATGAG NCA 2 0.0 Tier II
15 NC_000073.6 7 144612794 - Ano1 NNNAGGCCATGTCCCCTGAG NGG 2 0.0 Tier II
16 NC_000075.6 9 48418692 - Gm32131 NNNTGGCCATCTCCCATGAG NTG 2 0.0248 Tier III
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Other clones with same target sequence:

(none)