Construct: sgRNA BRDN0001162522
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGAACGAAGACGCACACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK10 (152110)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75635
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
27192098 |
- |
NEK10 |
NNNAACGAAGACGCACACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
15446576 |
+ |
CDRT4 |
NNNAACGAACACACACACAA |
NGG |
2 |
0.3692 |
Tier II |
| 3 |
NC_000017.11 |
17 |
15446576 |
+ |
TVP23C-CDRT4 |
NNNAACGAACACACACACAA |
NGG |
2 |
0.3692 |
Tier II |
| 4 |
NC_000010.11 |
10 |
113132101 |
- |
TCF7L2 |
NNNAAGGAAGACGAACACAA |
NGG |
2 |
0.175 |
Tier II |
| 5 |
NC_000012.12 |
12 |
15209408 |
- |
RERG |
NNNAATGAAGACGCACACAA |
NTG |
2 |
0.0362 |
Tier II |
| 6 |
NC_000023.11 |
X |
17429067 |
+ |
NHS |
NNNAACGAAGACGCACACAC |
NTG |
2 |
0.0089 |
Tier II |
| 7 |
NC_000015.10 |
15 |
58770983 |
+ |
MINDY2-DT |
NNNAAAGAAGCCGCACACAA |
NGG |
2 |
0.3714 |
Tier III |
| 8 |
NC_000007.14 |
7 |
112967 |
- |
LOC105375114 |
NNNAAAGAAGACGGACACAA |
NGG |
2 |
0.0 |
Tier III |
| 9 |
NC_000004.12 |
4 |
147578071 |
- |
LINC02507 |
NNNAACCAAGACGGACACAA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
123127013 |
+ |
Rhof |
NNNAACCAAGACGCAGACAA |
NGG |
2 |
0.1058 |
Tier II |
| 2 |
NC_000069.6 |
3 |
155172680 |
+ |
Lrriq3 |
NNNCACGAAGACGCACAGAA |
NGG |
2 |
0.0471 |
Tier II |
| 3 |
NC_000077.6 |
11 |
26167739 |
- |
5730522E02Rik |
NNNAACAAATACGCACACAA |
NGG |
2 |
0.8125 |
Tier III |
Other clones with same target sequence:
(none)
