Construct: sgRNA BRDN0001162524
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCCCGGACCCCGAATATGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK1 (27102)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77050
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
6058971 |
- |
EIF2AK1 |
NNNCCGGACCCCGAATATGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
12747857 |
- |
CAMK1D |
NNNCCCCACCCCGAATATGA |
NGG |
2 |
0.4687 |
Tier II |
| 3 |
NC_000002.12 |
2 |
233179578 |
+ |
INPP5D |
NNNCCGGGCCCGGAATATGA |
NGG |
2 |
0.3259 |
Tier II |
| 4 |
NC_000006.12 |
6 |
83386740 |
+ |
ME1 |
NNNCCTGACCCCCAATATGA |
NGG |
2 |
0.2807 |
Tier II |
| 5 |
NC_000004.12 |
4 |
15978145 |
+ |
PROM1 |
NNNCCGGACACCGAATCTGA |
NGG |
2 |
0.1529 |
Tier II |
| 6 |
NC_000008.11 |
8 |
4233435 |
+ |
CSMD1 |
NNNCCTGACCCCGAATGTGA |
NGG |
2 |
0.1176 |
Tier II |
| 7 |
NC_000022.11 |
22 |
34913539 |
- |
LINC02885 |
NNNCCTGACCCCGAATATGA |
NGG |
1 |
0.6667 |
Tier III |
| 8 |
NC_000011.10 |
11 |
102808044 |
- |
CSNK1A1P2 |
NNNCCGGGCCGCGAATATGA |
NGG |
2 |
0.1833 |
Tier III |
| 9 |
NC_000011.10 |
11 |
102808044 |
- |
WTAPP1 |
NNNCCGGGCCGCGAATATGA |
NGG |
2 |
0.1833 |
Tier III |
| 10 |
NC_000002.12 |
2 |
176638100 |
+ |
LINC01117 |
NNNCCGGACCCCGAATCTGA |
NAG |
2 |
0.0458 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
86287763 |
- |
Gpatch2l |
NNNCTGGACCCCCAATATGA |
NGG |
2 |
0.2679 |
Tier II |
Other clones with same target sequence:
(none)
