Construct: sgRNA BRDN0001162525

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGCCAAACCCACGACCTCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUAK1 (9891)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75578

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 106067716 - NUAK1 NNNCAAACCCACGACCTCTG NGG 0 1.0 Tier I
2 NC_000013.11 13 107191173 - NALF1 NNNCAAACCAATGACCTCTG NGG 2 0.4667 Tier II
3 NC_000021.9 21 34835141 + RUNX1 NNNCAAACCCACAGCCTCTG NGG 2 0.4308 Tier II
4 NC_000003.12 3 99865637 - FILIP1L NNNCAAACCCACCACCTCTG NGG 1 0.4211 Tier II
5 NC_000003.12 3 99865637 - CMSS1 NNNCAAACCCACCACCTCTG NGG 1 0.4211 Tier II
6 NC_000003.12 3 158812237 + MFSD1 NNNTAAACCCAGGACCTCTG NGG 2 0.3556 Tier II
7 NC_000002.12 2 119944309 + PTPN4 NNNCAAATCCACGACTTCTG NGG 2 0.2692 Tier II
8 NC_000010.11 10 43472313 + ZNF487 NNNCAAACCCACAACCTCTG NAG 2 0.2393 Tier II
9 NC_000019.10 19 46974739 - ARHGAP35 NNNCCCACCCACGACCTCTG NGG 2 0.2273 Tier II
10 NC_000011.10 11 132904847 - OPCML NNNCAAACTCACGACCTCTG NAG 2 0.2269 Tier II
11 NC_000014.9 14 74021005 + BBOF1 NNNCAAACCCATTACCTCTG NGG 2 0.1615 Tier II
12 NC_000002.12 2 8805344 - KIDINS220 NNNCTAACCCACCACCTCTG NGG 2 0.1531 Tier II
13 NC_000007.14 7 66144934 - CRCP NNNCAAACCCTCGACCTCAG NGG 2 0.0879 Tier II
14 NC_000007.14 7 1678704 - ELFN1 NNNCACACCCACGACCGCTG NGG 2 0.0535 Tier II
15 NC_000001.11 1 1341849 - DVL1 NNNCAAACCCATGACCTCTG NGA 2 0.0374 Tier II
16 NC_000010.11 10 84435969 - CCSER2 NNNCAAACCCACAACCTCTG NGT 2 0.0149 Tier II
17 NC_000012.12 12 109755561 + FAM222A NNNCAAACCCACGACCTCCG NTG 2 0.0107 Tier II
18 NC_000003.12 3 99865637 - LOC105374010 NNNCAAACCCACCACCTCTG NGG 1 0.4211 Tier III
19 NC_000014.9 14 19340081 - LINC01297-DUXAP10-NBEAP6 NNNCACACCCAGGACCTCTG NGG 2 0.202 Tier III
20 NC_000015.10 15 81749644 - LOC124903541 NNNCAAACCCACAATCTCTG NGG 2 0.0615 Tier III
21 NC_000008.11 8 87789637 - SOX5P1 NNNCAAACCCAAGACCTCTG NTG 2 0.0278 Tier III
22 NC_000005.10 5 24303254 - LOC105374689 NNNCAAACCCATGACCTCTG NTG 2 0.021 Tier III
23 NC_000007.14 7 35344231 + LOC401324 NNNCAAACCCACTATCTCTG NGG 2 0.02 Tier III
24 NC_000012.12 12 121089362 + LOC105378258 NNNCAAACCCACGTCCTCTG NGC 2 0.0119 Tier III
25 NC_000012.12 12 109755561 + FAM222A-AS1 NNNCAAACCCACGACCTCCG NTG 2 0.0107 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 29071106 - Lep NNNCAAAGCCACCACCTCTG NGG 2 0.2707 Tier I
2 NC_000083.6 17 75505160 - Rasgrp3 NNNCAAACCCAAGACATCTG NGG 2 0.7143 Tier II
3 NC_000070.6 4 44705914 + Pax5 NNNAAAACCCAAGACCTCTG NGG 2 0.6015 Tier II
4 NC_000083.6 17 10449020 + Pacrg NNNCAAACCCAAGACCTTTG NGG 2 0.4592 Tier II
5 NC_000068.7 2 52469753 - Cacnb4 NNNCAAAACCACGACCTTTG NGG 2 0.4179 Tier II
6 NC_000075.6 9 72983282 + Ccpg1os NNNCAAACTCAGGACCTCTG NGG 2 0.3889 Tier II
7 NC_000075.6 9 64999094 + Hacd3 NNNCAAACCCAAGTCCTCTG NGG 2 0.381 Tier II
8 NC_000076.6 10 121280573 + Tbc1d30 NNNCAACACCACGACCTCTG NGG 2 0.2844 Tier II
9 NC_000077.6 11 106402573 - Ern1 NNNCAAACCCAGGTCCTCTG NGG 2 0.237 Tier II
10 NC_000083.6 17 12189222 - Agpat4 NNNCAAACCCAGGTCCTCTG NGG 2 0.237 Tier II
11 NC_000075.6 9 114069767 - Susd5 NNNCCACCCCACGACCTCTG NGG 2 0.2188 Tier II
12 NC_000085.6 19 55397416 - Vti1a NNNCAAACCCAGGACCGCTG NGG 2 0.0523 Tier II
13 NC_000085.6 19 40260624 + Pdlim1 NNNCAAACCCAGGACCTCTG NCG 2 0.0476 Tier II
14 NC_000067.6 1 20509910 - Pkhd1 NNNCAAACCCAGGACCTCTG NGA 2 0.0309 Tier II
15 NC_000075.6 9 104840994 - Cpne4 NNNCAAACCCAGGACCTCTG NGA 2 0.0309 Tier II
16 NC_000078.6 12 91254784 + Cep128 NNNCAAACCCTCGACCTCTG NGC 2 0.0068 Tier II
17 NC_000073.6 7 102049242 + Rnf121 NNNCAAACCCACGACCTCTG NAA 2 0.0 Tier II
18 NC_000073.6 7 144554048 + Gm35626 NNNAAAACCCAAGACCTCTG NGG 2 0.6015 Tier III
19 NC_000077.6 11 96892987 + Gm38961 NNNCAAACCCAGGACCCCTG NGG 2 0.3137 Tier III
20 NC_000074.6 8 34436796 - Gm33910 NNNCAAACCCACGACCTCTG NAG 1 0.2593 Tier III
21 NC_000081.6 15 100444499 - 5330439K02Rik NNNCAAACCCAGGTCCTCTG NGG 2 0.237 Tier III
22 NC_000086.7 X 88756272 - Gm8876 NNNCACACCCACGGCCTCTG NGG 2 0.2121 Tier III
23 NC_000077.6 11 107007341 - Gm39424 NNNCAAACTCACGACCTCTG NGA 2 0.0608 Tier III
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Other clones with same target sequence:

(none)