Construct: sgRNA BRDN0001162535
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCGACGCACAGCTTCCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PTK6 (5753)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76493
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
63535033 |
+ |
PTK6 |
NNNGACGCACAGCTTCCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
98212866 |
+ |
BHLHA15 |
NNNGATCCACAGCTTCCGAG |
NGG |
2 |
0.6384 |
Tier I |
| 3 |
NC_000009.12 |
9 |
114473639 |
- |
WHRN |
NNNGAAGCACAGCTTCCCAG |
NGG |
2 |
0.4422 |
Tier II |
| 4 |
NC_000004.12 |
4 |
168398742 |
+ |
DDX60L |
NNNGACGACCAGCTTCCGAG |
NGG |
2 |
0.3714 |
Tier II |
| 5 |
NC_000017.11 |
17 |
42062602 |
+ |
ZNF385C |
NNNGACCCACAGATTCCGAG |
NGG |
2 |
0.2644 |
Tier II |
| 6 |
NC_000015.10 |
15 |
68782415 |
+ |
ANP32A |
NNNGACACACAGCTTGCGAG |
NGG |
2 |
0.1538 |
Tier II |
| 7 |
NC_000011.10 |
11 |
64184025 |
- |
LOC124902686 |
NNNGAGGCACAGCTGCCGAG |
NGG |
2 |
0.025 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
34815556 |
- |
Mylk |
NNNGACGCAGAGGTTCCGAG |
NGG |
2 |
0.053 |
Tier I |
| 2 |
NC_000071.6 |
5 |
51894315 |
- |
Ppargc1a |
NNNCACGCACAGCTTCCGAA |
NGG |
2 |
0.4963 |
Tier II |
| 3 |
NC_000067.6 |
1 |
75467097 |
- |
Asic4 |
NNNGCCGCACAGCTTCAGAG |
NGG |
2 |
0.2333 |
Tier II |
| 4 |
NC_000073.6 |
7 |
46472480 |
+ |
Sergef |
NNNGACGCACAGCTTCAGAG |
NAG |
2 |
0.121 |
Tier II |
| 5 |
NC_000072.6 |
6 |
145462001 |
+ |
Gm38475 |
NNNGGCGCACAGCTTCTGAG |
NGG |
2 |
0.336 |
Tier III |
Other clones with same target sequence:
(none)
