Construct: sgRNA BRDN0001162553
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGAGCAACTCACCGTAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERN2 (10595)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75847
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
23695962 |
- |
ERN2 |
NNNAGCAACTCACCGTAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
89670975 |
- |
MDN1 |
NNNAGCAACTCACCGTAGTA |
NTG |
2 |
0.0365 |
Tier I |
| 3 |
NC_000012.12 |
12 |
64043925 |
- |
SRGAP1 |
NNNAGCATCTCACAGTAGTG |
NGG |
2 |
0.15 |
Tier II |
| 4 |
NC_000014.9 |
14 |
99224004 |
- |
BCL11B |
NNNAGGAACTCACCGTGGTG |
NGG |
2 |
0.0882 |
Tier II |
| 5 |
NC_000006.12 |
6 |
89670975 |
- |
MDN1-AS1 |
NNNAGCAACTCACCGTAGTA |
NTG |
2 |
0.0365 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
58651435 |
+ |
Edar |
NNNAGGAACTCACCGGAGTG |
NGG |
2 |
0.1731 |
Tier I |
| 2 |
NC_000067.6 |
1 |
152658565 |
+ |
Rgl1 |
NNNAGCTACTCACCGTAGTG |
NGG |
1 |
0.4375 |
Tier II |
| 3 |
NC_000071.6 |
5 |
128864004 |
+ |
Rimbp2 |
NNNAGGAACTCACCGTGGTG |
NGG |
2 |
0.0882 |
Tier II |
| 4 |
NC_000083.6 |
17 |
87364503 |
+ |
Ttc7 |
NNNAGCAACTGACAGTAGTG |
NGG |
2 |
0.0875 |
Tier II |
| 5 |
NC_000070.6 |
4 |
132312827 |
- |
Trnau1ap |
NNNAGCAACTCAGCGTGGTG |
NGG |
2 |
0.0241 |
Tier II |
| 6 |
NC_000083.6 |
17 |
15516968 |
- |
Tbp |
NNNAGCAACTCACAGTAGTG |
NTG |
2 |
0.0136 |
Tier II |
| 7 |
NC_000068.7 |
2 |
110374378 |
- |
Gm30319 |
NNNAACAACTCACTGTAGTG |
NGG |
2 |
0.6356 |
Tier III |
| 8 |
NC_000068.7 |
2 |
163928034 |
- |
Gm35886 |
NNNAGGAACTCACAGTAGTG |
NGG |
2 |
0.175 |
Tier III |
| 9 |
NC_000083.6 |
17 |
87364503 |
+ |
LOC102632541 |
NNNAGCAACTGACAGTAGTG |
NGG |
2 |
0.0875 |
Tier III |
Other clones with same target sequence:
(none)
