Construct: sgRNA BRDN0001162558
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTGGTGCTTCCCGACCCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PKN1 (5585)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76278
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
14441429 |
+ |
PKN1 |
NNNGGTGCTTCCCGACCCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
27457896 |
+ |
IFT172 |
NNNGGTGCTTCCCTACCAGG |
NGG |
2 |
0.1436 |
Tier I |
| 3 |
NC_000003.12 |
3 |
53758605 |
+ |
CACNA1D |
NNNGGTGCTTCCTGACCCTG |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000003.12 |
3 |
45995554 |
+ |
FYCO1 |
NNNGGTGCTTCCCGGCCCGC |
NGG |
2 |
0.2786 |
Tier II |
| 5 |
NC_000014.9 |
14 |
102545892 |
+ |
LINC02323 |
NNNGGTGCTTCCCGACCCTG |
NTG |
2 |
0.026 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
85026932 |
- |
Trir |
NNNGGCGCCTCCCGACCCGG |
NGG |
2 |
0.8392 |
Tier I |
| 2 |
NC_000077.6 |
11 |
115814833 |
+ |
Tsen54 |
NNNGGTGCTACCCGGCCCGG |
NGG |
2 |
0.5571 |
Tier I |
| 3 |
NC_000085.6 |
19 |
28325775 |
+ |
Glis3 |
NNNGGTTCTTCCCGACCCGG |
NGG |
1 |
0.5714 |
Tier II |
| 4 |
NC_000083.6 |
17 |
74047402 |
+ |
Srd5a2 |
NNNGGTGCTTCCCAACCCTG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000071.6 |
5 |
66405938 |
- |
Apbb2 |
NNNGGTGCTTCTCGACCCGT |
NGG |
2 |
0.3769 |
Tier II |
| 6 |
NC_000074.6 |
8 |
107294235 |
- |
Nfat5 |
NNNGGTGATTCCCGAGCCGG |
NGG |
2 |
0.1 |
Tier II |
| 7 |
NC_000076.6 |
10 |
26486241 |
+ |
Gm30034 |
NNNGGTGCTTCCAGACCCTG |
NGG |
2 |
0.2564 |
Tier III |
| 8 |
NC_000071.6 |
5 |
137348042 |
+ |
Gm52791 |
NNNGGTGCTTCCTGACCCGG |
NGA |
2 |
0.0486 |
Tier III |
Other clones with same target sequence:
(none)
