Construct: sgRNA BRDN0001162560

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CGTCCTCAAAGTACACATCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEF2K (29904)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77856

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 22251241 - EEF2K NNNCCTCAAAGTACACATCC NGG 0 1.0 Tier I
2 NC_000002.12 2 223998183 + SERPINE2 NNNCCTCAAAGTTCACATTC NGG 2 0.1385 Tier I
3 NC_000011.10 11 8618931 + TRIM66 NNNCCTCAAAGAACACTTCC NGG 2 0.1067 Tier I
4 NC_000020.11 20 49233315 - DDX27 NNNCCTCAAAGTACTCATCC NGC 2 0.0044 Tier I
5 NC_000001.11 1 207909796 + CD34 NNNCCCCAAAGCACACATCC NGG 2 0.8612 Tier II
6 NC_000007.14 7 29159709 + CHN2 NNNCCTCACAGCACACATCC NGG 2 0.5414 Tier II
7 NC_000007.14 7 29159709 + CPVL NNNCCTCACAGCACACATCC NGG 2 0.5414 Tier II
8 NC_000011.10 11 16973143 - PLEKHA7 NNNCATAAAAGTACACATCC NGG 2 0.4286 Tier II
9 NC_000007.14 7 2816029 + GNA12 NNNCGTCTAAGTACACATCC NGG 2 0.2571 Tier II
10 NC_000011.10 11 133381523 - OPCML NNNCCTCAAGCTACACATCC NGG 2 0.2381 Tier II
11 NC_000018.10 18 35051638 + MAPRE2 NNNCCTCAAAGTGCACAGCC NGG 2 0.2174 Tier II
12 NC_000009.12 9 15191559 + TTC39B NNNCCTCAAAGCCCACATCC NGG 2 0.1994 Tier II
13 NC_000009.12 9 132455867 - CFAP77 NNNCCTCAAAGCCCACATCC NGG 2 0.1994 Tier II
14 NC_000018.10 18 48556513 + CTIF NNNCCTCAAAGTACACACTC NGG 2 0.1978 Tier II
15 NC_000005.10 5 45687794 + HCN1 NNNCCTCAATGTACACATGC NGG 2 0.1103 Tier II
16 NC_000016.10 16 6968967 - RBFOX1 NNNCCTCAAAGTACACATCC NGA 1 0.0694 Tier II
17 NC_000007.14 7 70401336 - AUTS2 NNNCCTCAAACTACACATGC NGG 2 0.0536 Tier II
18 NC_000004.12 4 65245471 + LOC124900852 NNNCCTCAAAGAATACATCC NGG 2 0.5867 Tier III
19 NC_000014.9 14 50962242 + LOC400212 NNNCCTCAAGATACACATCC NGG 2 0.5556 Tier III
20 NC_000002.12 2 104403308 + LOC100287010 NNNCACCAAAGTACACATCC NGG 2 0.5195 Tier III
21 NC_000001.11 1 94820105 + SLC44A3-AS1 NNNCGTCAAAGTGCACATCC NGG 2 0.3913 Tier III
22 NC_000020.11 20 48558340 - LOC105372646 NNNCCTCAAAGTGCACATTC NGG 2 0.301 Tier III
23 NC_000002.12 2 39936394 + SLC8A1-AS1 NNNCCTCAAAGTACAAATCC NAG 2 0.2593 Tier III
24 NC_000014.9 14 97743933 + LOC105370651 NNNCCTCAAAGTACACATCA NGC 2 0.0111 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 7254445 + Egflam NNNCCTCAAAGGATACATCC NGG 2 0.3667 Tier I
2 NC_000067.6 1 75491011 + Obsl1 NNNCCTCAAGGTGCACATCC NGG 2 0.3623 Tier I
3 NC_000070.6 4 128604354 - Zscan20 NNNCCTCAAAGAACAGATCC NGG 2 0.1231 Tier I
4 NC_000067.6 1 90931523 - Mlph NNNCCTCAAAGTCCACATCT NGG 2 0.0632 Tier I
5 NC_000086.7 X 159366692 - Rps6ka3 NNNGCTCAAAGTATACATCC NGG 2 0.3667 Tier II
6 NC_000076.6 10 3823976 + Plekhg1 NNNCCCCAAATTACACATCC NGG 2 0.3497 Tier II
7 NC_000075.6 9 9180944 + Arhgap42 NNNCCTCACAGTACACATCA NGG 2 0.2857 Tier II
8 NC_000084.6 18 44538297 - Mcc NNNCCTCAGAGTACACACCC NGG 2 0.2857 Tier II
9 NC_000075.6 9 13570312 + Maml2 NNNCCTCAAAGCTCACATCC NGG 2 0.2842 Tier II
10 NC_000067.6 1 74775942 - Wnt6 NNNCATCAAAGTACACATTC NGG 2 0.2637 Tier II
11 NC_000069.6 3 142819543 - Pkn2 NNNCTTCAAAGTAAACATCC NGG 2 0.2227 Tier II
12 NC_000079.6 13 47210653 + Rnf144b NNNCCTCAAAGTACACACCA NGG 2 0.2143 Tier II
13 NC_000077.6 11 97217743 + Npepps NNNCCTCTAAGTACACATCC NAG 2 0.1111 Tier II
14 NC_000069.6 3 142740110 + Kyat3 NNNCCTCAAAGGACAGATCC NGG 2 0.0769 Tier II
15 NC_000074.6 8 113734236 + Adamts18 NNNCCTCAAAGTCCATATCC NGG 2 0.0648 Tier II
16 NC_000076.6 10 73397125 - Pcdh15 NNNCCTCAAAGTAAACTTCC NGG 2 0.0467 Tier II
17 NC_000081.6 15 82931547 - Tcf20 NNNCCTCAAAGTTCACTTCC NGG 2 0.04 Tier II
18 NC_000085.6 19 10972834 + Ms4a10 NNNCCTCAAAGTCCACCTCC NGG 2 0.0372 Tier II
19 NC_000076.6 10 43247179 - Pdss2 NNNCCTCAAAGTCCACTTCC NGG 2 0.0281 Tier II
20 NC_000081.6 15 30186333 + Ctnnd2 NNNCCTCAAAGTACACATTC NGT 2 0.0074 Tier II
21 NC_000082.6 16 14103276 + Bmerb1 NNNCCTCAAAGTCCACATCC NGT 2 0.0034 Tier II
22 NC_000077.6 11 82857807 + Rffl NNNCCTCAAAGTACACATCC NAA 2 0.0 Tier II
23 NC_000080.6 14 120362464 + Mbnl2 NNNCCTGAAAGTAGACATCC NGG 2 0.0 Tier II
24 NC_000069.6 3 64739279 + Vmn2r-ps11 NNNCCTAAAAGGACACATCC NGG 2 0.375 Tier III
25 NC_000076.6 10 127842929 - Rdh18-ps NNNCCTAAAAGTACACATTC NGG 2 0.3462 Tier III
26 NC_000084.6 18 44538297 - Gm52347 NNNCCTCAGAGTACACACCC NGG 2 0.2857 Tier III
27 NC_000067.6 1 74775942 - Gm29539 NNNCATCAAAGTACACATTC NGG 2 0.2637 Tier III
28 NC_000067.6 1 73145784 - Gm31197 NNNCCTCAAAGTTAACATCC NGG 2 0.105 Tier III
29 NC_000067.6 1 73145784 - Gm29185 NNNCCTCAAAGTTAACATCC NGG 2 0.105 Tier III
30 NC_000081.6 15 30186333 + Gm31953 NNNCCTCAAAGTACACATTC NGT 2 0.0074 Tier III
31 NC_000073.6 7 120841794 + Gm36449 NNNCCTCAAAGTACACATGC NTG 2 0.0049 Tier III
32 NC_000070.6 4 56712426 + Gm34687 NNNCCTCAAAGTTCACATCC NGT 2 0.0048 Tier III
33 NC_000080.6 14 120362464 + Gm26679 NNNCCTGAAAGTAGACATCC NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)