Construct: sgRNA BRDN0001162561
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGGCCCTCAGGTGTCCGGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDC42BPG (55561)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77527
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
64832721 |
- |
CDC42BPG |
NNNCCCTCAGGTGTCCGGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
3046288 |
+ |
MRPS26 |
NNNCCCTCAGGTGTGCGGCC |
NGG |
2 |
0.0214 |
Tier I |
| 3 |
NC_000019.10 |
19 |
48451245 |
- |
GRWD1 |
NNNCCCCCAGGTGTCCGGCT |
NGG |
2 |
0.4812 |
Tier II |
| 4 |
NC_000017.11 |
17 |
62478584 |
+ |
TLK2 |
NNNCCCTCAGCCGTCCGGCG |
NGG |
2 |
0.406 |
Tier II |
| 5 |
NC_000009.12 |
9 |
345736 |
+ |
DOCK8 |
NNNCCTTCAGGTGTCCTGCG |
NGG |
2 |
0.2321 |
Tier II |
| 6 |
NC_000010.11 |
10 |
5933556 |
+ |
FBH1 |
NNNCCCTCAGGGGTCCTGCG |
NGG |
2 |
0.125 |
Tier II |
| 7 |
NC_000020.11 |
20 |
36220221 |
- |
EPB41L1 |
NNNCCCTCAGGTGTCCTGCC |
NGG |
2 |
0.1071 |
Tier II |
| 8 |
NC_000012.12 |
12 |
460674 |
+ |
B4GALNT3 |
NNNCCCTCAGGTGCCCGGCG |
NCG |
2 |
0.0306 |
Tier II |
| 9 |
NC_000016.10 |
16 |
2955282 |
- |
LOC105371055 |
NNNCCCACAGGTGTCAGGCG |
NGG |
2 |
0.875 |
Tier III |
| 10 |
NC_000020.11 |
20 |
36220221 |
- |
LOC105372602 |
NNNCCCTCAGGTGTCCTGCC |
NGG |
2 |
0.1071 |
Tier III |
| 11 |
NC_000015.10 |
15 |
31224129 |
- |
LINC03034 |
NNNCCCTCAGGTGTCTCGCG |
NGG |
2 |
0.0724 |
Tier III |
| 12 |
NC_000015.10 |
15 |
31224129 |
- |
LINC02352 |
NNNCCCTCAGGTGTCTCGCG |
NGG |
2 |
0.0724 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
6317398 |
+ |
Cdc42bpg |
NNNCCCTCAGGTGTCCGTCG |
NGG |
1 |
0.6667 |
Tier I |
| 2 |
NC_000077.6 |
11 |
65221035 |
+ |
Myocd |
NNNCCCTCGGGTGTCCGGCG |
NTG |
2 |
0.026 |
Tier II |
Other clones with same target sequence:
(none)
