Construct: sgRNA BRDN0001162568

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCGCACTCACTCACCCACGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKX (5613)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78013

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 3713091 + PRKX NNNCACTCACTCACCCACGG NGG 0 1.0 Tier I
2 NC_000007.14 7 75986239 - POR NNNCACTCACTCACCCACAG NCG 2 0.0765 Tier I
3 NC_000006.12 6 169234737 + THBS2 NNNCCCTCACTCACCCACGG NGC 2 0.0111 Tier I
4 NC_000003.12 3 48649475 + CELSR3 NNNCACACACTCACCCACGT NGG 2 0.6125 Tier II
5 NC_000002.12 2 134151757 + MGAT5 NNNCACTCACTCATCCACTG NGG 2 0.4889 Tier II
6 NC_000002.12 2 177989533 + PDE11A NNNCACTCACTGACCCACTG NGG 2 0.2963 Tier II
7 NC_000002.12 2 50198834 - NRXN1 NNNCAGTCACTCACCCAAGG NGG 2 0.2692 Tier II
8 NC_000005.10 5 176458454 + FAF2 NNNCACTCTCTCACCCACGC NGG 2 0.2571 Tier II
9 NC_000006.12 6 163253274 - PACRG NNNCACTCTCTCACCCACGC NGG 2 0.2571 Tier II
10 NC_000016.10 16 25252892 - ZKSCAN2 NNNCACTCTGTCACCCACGG NGG 2 0.2333 Tier II
11 NC_000005.10 5 54069692 + ARL15 NNNCACTCAGTCACCCACGC NGG 2 0.1667 Tier II
12 NC_000019.10 19 47551902 + ZNF541 NNNCACTCAGTCACCCACGC NGG 2 0.1667 Tier II
13 NC_000013.11 13 112397692 + SPACA7 NNNCGCTCACTCACACACGG NGG 2 0.16 Tier II
14 NC_000009.12 9 37736830 - FRMPD1 NNNCACTCACTCACACACAG NGG 2 0.1587 Tier II
15 NC_000012.12 12 123898867 - DNAH10 NNNCCCTCACTCACCTACGG NGG 2 0.1538 Tier II
16 NC_000018.10 18 45519541 + SLC14A2 NNNCACTCACTCACCCCTGG NGG 2 0.1134 Tier II
17 NC_000020.11 20 20395021 + RALGAPA2 NNNCACCCACTCACCCAGGG NGG 2 0.0917 Tier II
18 NC_000005.10 5 79318046 + JMY NNNCACTCTCTCACCCAGGG NGG 2 0.08 Tier II
19 NC_000006.12 6 13105353 - PHACTR1 NNNCACTCTCTCACCCAGGG NGG 2 0.08 Tier II
20 NC_000007.14 7 107545754 - COG5 NNNCACTCTCTCACCCAGGG NGG 2 0.08 Tier II
21 NC_000011.10 11 84244227 + DLG2 NNNCACTCTCTCACCCAGGG NGG 2 0.08 Tier II
22 NC_000023.11 X 32706538 - DMD NNNCACTCTCTCACCCAGGG NGG 2 0.08 Tier II
23 NC_000003.12 3 13002521 - IQSEC1 NNNCACTCACTCACCCAGGC NGG 2 0.0571 Tier II
24 NC_000009.12 9 114438209 - WHRN NNNCACTCACTCACCCAGGC NGG 2 0.0571 Tier II
25 NC_000010.11 10 95706073 - ENTPD1 NNNCACTCACTCACCCAGGC NGG 2 0.0571 Tier II
26 NC_000010.11 10 96877498 - LCOR NNNCACTCACTCACCCAGGC NGG 2 0.0571 Tier II
27 NC_000012.12 12 118378526 + SUDS3 NNNCACTCACTCACCCAGGC NGG 2 0.0571 Tier II
28 NC_000021.9 21 32590214 - CFAP298-TCP10L NNNCACTCACTCACCCAGGC NGG 2 0.0571 Tier II
29 NC_000013.11 13 71484206 + DACH1 NNNCACTCAGTCACCCAGGG NGG 2 0.0519 Tier II
30 NC_000020.11 20 4965941 - SLC23A2 NNNCACTCAGTCACCCAGGG NGG 2 0.0519 Tier II
31 NC_000011.10 11 126726949 + KIRREL3 NNNCACTCACTCACACAGGG NGG 2 0.0296 Tier II
32 NC_000009.12 9 474138 - KANK1 NNNCACTCACTCACCCACTG NTG 2 0.026 Tier II
33 NC_000010.11 10 12371218 + CAMK1D NNNCACTCACTCACCCAGGG NGA 2 0.0093 Tier II
34 NC_000004.12 4 140359276 + SCOC NNNCACTCACACAGCCACGG NGG 2 0.0 Tier II
35 NC_000002.12 2 46729672 - SOCS5 NNNCACTCACTCACCCACGG NAA 2 0.0 Tier II
36 NC_000004.12 4 186922777 + LOC102723906 NNNCACTCATTCACCCACTG NGG 2 0.6275 Tier III
37 NC_000009.12 9 103261297 + LINC01492 NNNCACTCTATCACCCACGG NGG 2 0.52 Tier III
38 NC_000012.12 12 131459457 + LOC101929974 NNNCACCCACCCACCCACGG NGG 2 0.4583 Tier III
39 NC_000017.11 17 27987571 - LINC01992 NNNCACTCTGTCACCCACGG NGG 2 0.2333 Tier III
40 NC_000013.11 13 112397692 + LOC105370372 NNNCGCTCACTCACACACGG NGG 2 0.16 Tier III
41 NC_000005.10 5 79318046 + LOC102724530 NNNCACTCTCTCACCCAGGG NGG 2 0.08 Tier III
42 NC_000023.11 X 3843344 + LOC389906 NNNCACTCACTCACCCACGG NGA 1 0.0694 Tier III
43 NC_000023.11 X 3881768 + FAM239A NNNCACTCACTCACCCACGG NGA 1 0.0694 Tier III
44 NC_000023.11 X 3920197 + FAM239B NNNCACTCACTCACCCACGG NGA 1 0.0694 Tier III
45 NC_000022.11 22 26685184 - MIATNB NNNCACTCACTCACCCAGGC NGG 2 0.0571 Tier III
46 NC_000019.10 19 29381262 - VSTM2B-DT NNNCACTCAGTCACCCAGGG NGG 2 0.0519 Tier III
47 NC_000024.10 Y 7274476 - PRKY NNNCACTCACTCACCCATGG NGA 2 0.0446 Tier III
48 NC_000010.11 10 3236221 - LINC02668 NNNCACTCACTCACCCACCG NGA 2 0.0311 Tier III
49 NC_000017.11 17 73748679 + LOC100134391 NNNCACTCACTCACTCACGG NAG 2 0.0173 Tier III
50 NC_000006.12 6 169234737 + THBS2-AS1 NNNCCCTCACTCACCCACGG NGC 2 0.0111 Tier III
51 NC_000013.11 13 34454559 + LINC00457 NNNCACTCACTCACCCATGG NGT 2 0.0104 Tier III
52 NC_000013.11 13 34454559 + LINC02343 NNNCACTCACTCACCCATGG NGT 2 0.0104 Tier III
53 NC_000011.10 11 65216494 - SLC22A20P NNNCACTCACTCACCCACCG NGC 2 0.01 Tier III
54 NC_000004.12 4 140359276 + SCOC-AS1 NNNCACTCACACAGCCACGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 29232165 + Kcnk6 NNNCACTCACTTACCCACGG NGG 1 0.5385 Tier I
2 NC_000086.7 X 77795551 + Prkx NNNTTCTCACTCACCCACGG NGG 2 0.2909 Tier I
3 NC_000076.6 10 77918087 + Trpm2 NNNCACTCACTCTCCCACGG NGT 2 0.0048 Tier I
4 NC_000075.6 9 67220861 - Tln2 NNNCACTGACTCACCCACGG NGG 1 0.6429 Tier II
5 NC_000081.6 15 83846108 - Mpped1 NNNCACTCACACACCCACTG NGG 2 0.5 Tier II
6 NC_000070.6 4 155997813 - Sdf4 NNNCACTCACCCACCCACAG NGG 2 0.4762 Tier II
7 NC_000080.6 14 62350776 - Rnaseh2b NNNCAGACACTCACCCACGG NGG 2 0.4375 Tier II
8 NC_000070.6 4 44799215 + Zcchc7 NNNCACGCACTCACCCACGT NGG 2 0.4118 Tier II
9 NC_000080.6 14 52279797 + Tox4 NNNCGCTCACTCACCCACCG NGG 2 0.3228 Tier II
10 NC_000067.6 1 58431940 + Ppil3 NNNCACGCACGCACCCACGG NGG 2 0.2353 Tier II
11 NC_000069.6 3 51546535 - Setd7 NNNCACACACTCACACACGG NGG 2 0.1944 Tier II
12 NC_000082.6 16 20619227 + Camk2n2 NNNCACACACTCACACACGG NGG 2 0.1944 Tier II
13 NC_000082.6 16 20619227 + Eef1akmt4-ece2 NNNCACACACTCACACACGG NGG 2 0.1944 Tier II
14 NC_000074.6 8 111392977 + Fa2h NNNCACTCACTCACCCACAG NAG 2 0.1852 Tier II
15 NC_000067.6 1 182594802 - Capn8 NNNCACTCACACACACACGG NGG 2 0.1667 Tier II
16 NC_000075.6 9 97770688 - Clstn2 NNNCACTCACACACACACGG NGG 2 0.1667 Tier II
17 NC_000086.7 X 53144667 - Plac1 NNNCACTCACACACACACGG NGG 2 0.1667 Tier II
18 NC_000067.6 1 105807632 + Tnfrsf11a NNNCACTCACTCACCCCCAG NGG 2 0.1261 Tier II
19 NC_000083.6 17 45543929 + Tmem151b NNNCACTCACTCACCCCTGG NGG 2 0.1134 Tier II
20 NC_000071.6 5 140127888 + Mad1l1 NNNCACTCACTCACACACGC NGG 2 0.0952 Tier II
21 NC_000081.6 15 77751752 - Apol8 NNNCACTCACTCACTCACGG NGG 1 0.0667 Tier II
22 NC_000070.6 4 46673104 - Gabbr2 NNNCACACACTCACTCACGG NGG 2 0.0583 Tier II
23 NC_000081.6 15 98819250 + Prkag1 NNNCACTCACTCACTCACAG NGG 2 0.0476 Tier II
24 NC_000078.6 12 75143685 + Kcnh5 NNNCACTCACTCACTCACTG NGG 2 0.0444 Tier II
25 NC_000067.6 1 162042222 + Dnm3 NNNCACTCACTCACTCATGG NGG 2 0.0429 Tier II
26 NC_000069.6 3 95130167 + Scnm1 NNNCACTCACTCACCTTCGG NGG 2 0.041 Tier II
27 NC_000072.6 6 137829246 - Dera NNNCACTCACTCACACTCGG NGG 2 0.0296 Tier II
28 NC_000076.6 10 30782674 + Ncoa7 NNNCACTCACTCACCCACTG NTG 2 0.026 Tier II
29 NC_000070.6 4 135931222 + Fuca1 NNNCACTCACTCACTCACGG NAG 2 0.0173 Tier II
30 NC_000083.6 17 86492762 - Prkce NNNCACTCACTCACTCGCGG NGG 2 0.0118 Tier II
31 NC_000080.6 14 27302788 - Arhgef3 NNNCACTCACTCACCCACAG NGT 2 0.0115 Tier II
32 NC_000074.6 8 14283529 - Dlgap2 NNNCACTCACTCACACACGG NGT 2 0.0036 Tier II
33 NC_000080.6 14 118896333 + Dzip1 NNNCACTCACTCACCCTCGG NGC 2 0.003 Tier II
34 NC_000081.6 15 83846108 - Gm41370 NNNCACTCACACACCCACTG NGG 2 0.5 Tier III
35 NC_000067.6 1 90253899 - Gm34930 NNNCACCCACCCACCCACGG NGG 2 0.4583 Tier III
36 NC_000070.6 4 120234366 - Gm36134 NNNCACTCACTCACCCACAC NGG 2 0.3061 Tier III
37 NC_000069.6 3 37172619 - Gm36000 NNNCACTCACACACACACGG NGG 2 0.1667 Tier III
38 NC_000086.7 X 53144667 - Gm35988 NNNCACTCACACACACACGG NGG 2 0.1667 Tier III
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Other clones with same target sequence:

(none)