Construct: sgRNA BRDN0001162576
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGAGTGTCACCACACGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK9 (1025)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77170
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
127788344 |
- |
CDK9 |
NNNGAGTGTCACCACACGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
1117131 |
- |
SBNO2 |
NNNGAGTGTGACCACACAGT |
NGG |
2 |
0.2692 |
Tier II |
| 3 |
NC_000006.12 |
6 |
118184185 |
+ |
SLC35F1 |
NNNGAGTGTCTCCCCACGGT |
NGG |
2 |
0.0659 |
Tier II |
| 4 |
NC_000015.10 |
15 |
64096445 |
- |
SNX1 |
NNNGAGTGTCACCACACTGC |
NGG |
2 |
0.0606 |
Tier II |
| 5 |
NC_000002.12 |
2 |
110794694 |
+ |
ACOXL |
NNNGAGTGTCACCAAACGGT |
NTG |
2 |
0.0087 |
Tier II |
| 6 |
NC_000001.11 |
1 |
10679770 |
+ |
CASZ1 |
NNNGAGTGTCACCACAGGGT |
NCG |
2 |
0.0063 |
Tier II |
| 7 |
NC_000002.12 |
2 |
146228830 |
+ |
LOC105373667 |
NNNGAGTGTCACCACAGAGT |
NGG |
2 |
0.0407 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
32709815 |
+ |
Cdk9 |
NNNCAATGTCACCACACGGT |
NGG |
2 |
0.5294 |
Tier I |
| 2 |
NC_000070.6 |
4 |
44641907 |
+ |
Pax5 |
NNNAAGTGTCACCACACAGT |
NGG |
2 |
0.6231 |
Tier II |
| 3 |
NC_000070.6 |
4 |
156214058 |
- |
Perm1 |
NNNCAATGTCACCACACGGT |
NGG |
2 |
0.5294 |
Tier II |
| 4 |
NC_000068.7 |
2 |
112502923 |
- |
Aven |
NNNGAGTGGCACCACACAGT |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000068.7 |
2 |
112502923 |
- |
Chrm5 |
NNNGAGTGGCACCACACAGT |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000085.6 |
19 |
46870101 |
- |
Cnnm2 |
NNNCAGTGTCACCACACAGT |
NGG |
2 |
0.3665 |
Tier II |
| 7 |
NC_000084.6 |
18 |
12203970 |
+ |
Npc1 |
NNNTGGTGTCACCACACGGT |
NGG |
2 |
0.2618 |
Tier II |
| 8 |
NC_000070.6 |
4 |
156214058 |
- |
Gm46877 |
NNNCAATGTCACCACACGGT |
NGG |
2 |
0.5294 |
Tier III |
Other clones with same target sequence:
(none)
