Construct: sgRNA BRDN0001162582

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAAGTTTGGGAAAATCCTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDPK1 (5170)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77450

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 2557941 + PDPK1 NNNGTTTGGGAAAATCCTTG NGG 0 1.0 Tier I
2 NC_000016.10 16 77190359 - LOC124903725 NNNGTTTGGGAAAAGGCTTG NGG 2 0.0077 Tier I
3 NC_000016.10 16 16093859 + ABCC1 NNNGTTTGGGAGAATCCTTG NGG 1 0.7222 Tier II
4 NC_000023.11 X 11559524 - ARHGAP6 NNNGTTTGGGAAAAAACTTG NGG 2 0.5789 Tier II
5 NC_000002.12 2 133472693 - NCKAP5 NNNGTTTGGGAAAATATTTG NGG 2 0.4667 Tier II
6 NC_000019.10 19 55287672 - BRSK1 NNNGTTTGGGAAGATCCTTT NGG 2 0.4565 Tier II
7 NC_000006.12 6 12895840 - PHACTR1 NNNGTTCTGGAAAATCCTTG NGG 2 0.4297 Tier II
8 NC_000012.12 12 50635465 - DIP2B NNNGTTTGGGAAAAACCTTT NGG 2 0.4053 Tier II
9 NC_000012.12 12 80352837 - OTOGL NNNGTTTTAGAAAATCCTTG NGG 2 0.4018 Tier II
10 NC_000002.12 2 221545631 - EPHA4 NNNGTTTCAGAAAATCCTTG NGG 2 0.3956 Tier II
11 NC_000014.9 14 37531710 + MIPOL1 NNNGTTTTTGAAAATCCTTG NGG 2 0.3333 Tier II
12 NC_000014.9 14 37532619 - MIPOL1 NNNGTTTCTGAAAATCCTTG NGG 2 0.3282 Tier II
13 NC_000020.11 20 15139796 + MACROD2 NNNGTTTGGGAAAATTCTTG NGG 1 0.3077 Tier II
14 NC_000020.11 20 18024347 + OVOL2 NNNGTGTGTGAAAATCCTTG NGG 2 0.3048 Tier II
15 NC_000002.12 2 85395803 - CAPG NNNGTTTGGGAAAATTCTTA NGG 2 0.2885 Tier II
16 NC_000023.11 X 15780138 + CA5B NNNGTTTGGGGAAATCCTTC NGG 2 0.2786 Tier II
17 NC_000001.11 1 115287412 + NGF NNNGTTTGGCAAAAACCTTG NGG 2 0.2316 Tier II
18 NC_000019.10 19 14583669 + CLEC17A NNNGATTGGCAAAATCCTTG NGG 2 0.2 Tier II
19 NC_000019.10 19 14587926 + CLEC17A NNNGATTGGCAAAATCCTTG NGG 2 0.2 Tier II
20 NC_000019.10 19 14592594 + CLEC17A NNNGATTGGCAAAATCCTTG NGG 2 0.2 Tier II
21 NC_000014.9 14 80805134 - CEP128 NNNGTTTGGGATAAACCTTG NGG 2 0.193 Tier II
22 NC_000006.12 6 161365723 - PRKN NNNGTTTGGGGAAATCCTAG NGG 2 0.1857 Tier II
23 NC_000007.14 7 78136582 - MAGI2 NNNGTTTGGTAAACTCCTTG NGG 2 0.1741 Tier II
24 NC_000003.12 3 10876710 + SLC6A11 NNNGTTTGGGAAAATCCAGG NGG 2 0.1667 Tier II
25 NC_000002.12 2 42445561 - KCNG3 NNNCTTTGGGAAAATCCTAG NGG 2 0.1513 Tier II
26 NC_000015.10 15 40535047 + CCDC32 NNNGTTTGGGAAAGTCCTGG NGG 2 0.1167 Tier II
27 NC_000004.12 4 46257394 + GABRA2 NNNGTTTGGGAAAATGATTG NGG 2 0.0718 Tier II
28 NC_000003.12 3 68169078 - TAFA1 NNNGTTTGGGAACATCCTGG NGG 2 0.0526 Tier II
29 NC_000001.11 1 178815742 - RALGPS2 NNNGTTTGGGAAAATGCTTG NAG 2 0.0399 Tier II
30 NC_000014.9 14 102189943 + WDR20 NNNGGTTGGGAAAATCCTTG NTG 2 0.0249 Tier II
31 NC_000011.10 11 39932766 + LOC105376637 NNNGTTTGGAAAAATCATTG NGG 2 0.4356 Tier III
32 NC_000016.10 16 2642252 - FLJ42627 NNNGTTTGGGAAAATTCTTG NGG 1 0.3077 Tier III
33 NC_000016.10 16 2642252 - PDPK2P NNNGTTTGGGAAAATTCTTG NGG 1 0.3077 Tier III
34 NC_000023.11 X 15780138 + CA5BP1-CA5B NNNGTTTGGGGAAATCCTTC NGG 2 0.2786 Tier III
35 NC_000001.11 1 115287412 + NGF-AS1 NNNGTTTGGCAAAAACCTTG NGG 2 0.2316 Tier III
36 NC_000010.11 10 31997548 + LOC107984219 NNNGTTTGGGAATTTCCTTG NGG 2 0.16 Tier III
37 NC_000001.11 1 219848417 + LOC105372926 NNNGTTTGGGAAAATTTTTG NGG 2 0.1436 Tier III
38 NC_000023.11 X 22580321 - PTCHD1-AS NNNATTTGGGAAAATCCTTG NTG 2 0.0351 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 24110871 - Pdpk1 NNNGTTTGGGAAAATTCTTG NCG 2 0.033 Tier I
2 NC_000069.6 3 68494248 + Schip1 NNNGTTTGGGAGAATCCTTG NGG 1 0.7222 Tier II
3 NC_000069.6 3 68494248 + Iqschfp NNNGTTTGGGAGAATCCTTG NGG 1 0.7222 Tier II
4 NC_000072.6 6 60793638 - Snca NNNCTTTAGGAAAATCCTTG NGG 2 0.5294 Tier II
5 NC_000074.6 8 14974085 + Arhgef10 NNNCTTTGGGAAAATCCTTA NGG 2 0.4963 Tier II
6 NC_000070.6 4 150101415 + Gpr157 NNNGTTGGGGAGAATCCTTG NGG 2 0.4248 Tier II
7 NC_000070.6 4 76053991 + Ptprd NNNCTTTTGGAAAATCCTTG NGG 2 0.3309 Tier II
8 NC_000067.6 1 33790850 + Zfp451 NNNGTTTGGAAATATCCTTG NGG 2 0.28 Tier II
9 NC_000083.6 17 36110618 - H2-T-ps NNNTTTGGGGAAAATCCTTG NGG 2 0.2139 Tier II
10 NC_000072.6 6 116114536 - Tmcc1 NNNGTTTGGGGAAATTCTTG NGG 2 0.2 Tier II
11 NC_000071.6 5 81743597 + Adgrl3 NNNGTTTGGGGACATCCTTG NGG 2 0.1368 Tier II
12 NC_000080.6 14 25726215 + Zcchc24 NNNGTTTGGGAAAGTGCTTG NGG 2 0.0718 Tier II
13 NC_000068.7 2 84993950 + Prg3 NNNGTTTGGGAAAATCCTGG NAG 2 0.0648 Tier II
14 NC_000070.6 4 102792268 + Sgip1 NNNGTTTGGGAAAATCCTAG NGA 2 0.0198 Tier II
15 NC_000082.6 16 55473560 - Gm45968 NNNGATTGGAAAAATCCTTG NGG 2 0.4667 Tier III
16 NC_000067.6 1 119905569 + Gm28209 NNNGTTTGGGAAAGTCCTTT NGG 2 0.3267 Tier III
17 NC_000086.7 X 153068021 + 9530051G07Rik NNNGTTTGGGAGAATCCTTG NGA 2 0.0502 Tier III
18 NC_000080.6 14 103765792 + Gm35197 NNNGTTTAGGAAAATCCTTG NTG 2 0.039 Tier III
19 NC_000077.6 11 118978783 - Gm35710 NNNCTTTGGGAAAATCCTTG NGT 2 0.0085 Tier III
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Other clones with same target sequence:

(none)