Construct: sgRNA BRDN0001162589
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCTCTGGCCTTGTAAACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK7 (1022)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76079
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
69235404 |
- |
CDK7 |
NNNTCTGGCCTTGTAAACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
85582450 |
- |
CCN1 |
NNNTCTGGCCTTGTAAAGGG |
NTG |
2 |
0.0052 |
Tier I |
| 3 |
NC_000016.10 |
16 |
86582968 |
- |
FOXL1 |
NNNTCTGTCCTTGTGAACGG |
NGG |
2 |
0.4063 |
Tier II |
| 4 |
NC_000007.14 |
7 |
50783636 |
- |
GRB10 |
NNNTGTGGCCTTGTAAACGG |
NAG |
2 |
0.1556 |
Tier II |
| 5 |
NC_000017.11 |
17 |
57521946 |
- |
MSI2 |
NNNTCTGGCCTTGAAAAGGG |
NGG |
2 |
0.0825 |
Tier II |
| 6 |
NC_000002.12 |
2 |
157211463 |
+ |
CDK7P1 |
NNNTCTGGCCTTGTAAACCG |
NGG |
1 |
0.4483 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
36958393 |
- |
Pknox2 |
NNNTCTGACCTTGTAAACAG |
NGG |
2 |
0.7143 |
Tier II |
| 2 |
NC_000086.7 |
X |
83849359 |
+ |
Dmd |
NNNTCTGTCCTTGTAAACTG |
NGG |
2 |
0.4167 |
Tier II |
| 3 |
NC_000068.7 |
2 |
102081640 |
+ |
Ldlrad3 |
NNNTGTGGCCTTGTAAAAGG |
NGG |
2 |
0.3231 |
Tier II |
| 4 |
NC_000081.6 |
15 |
85781856 |
+ |
Ppara |
NNNTCTTGCCTTGTAAAAGG |
NGG |
2 |
0.3077 |
Tier II |
| 5 |
NC_000068.7 |
2 |
5372990 |
+ |
Camk1d |
NNNTCTGGCTTTGTAAACGG |
NGC |
2 |
0.0209 |
Tier II |
| 6 |
NC_000068.7 |
2 |
128433448 |
+ |
Gm36154 |
NNNTCAGGCCGTGTAAACGG |
NGG |
2 |
0.3467 |
Tier III |
| 7 |
NC_000084.6 |
18 |
69172034 |
- |
Gm41768 |
NNNTCTGTCCTTTTAAACGG |
NGG |
2 |
0.1875 |
Tier III |
Other clones with same target sequence:
(none)
