Construct: sgRNA BRDN0001162589

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATCTCTGGCCTTGTAAACGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK7 (1022)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76079

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 69235404 - CDK7 NNNTCTGGCCTTGTAAACGG NGG 0 1.0 Tier I
2 NC_000001.11 1 85582450 - CCN1 NNNTCTGGCCTTGTAAAGGG NTG 2 0.0052 Tier I
3 NC_000016.10 16 86582968 - FOXL1 NNNTCTGTCCTTGTGAACGG NGG 2 0.4063 Tier II
4 NC_000007.14 7 50783636 - GRB10 NNNTGTGGCCTTGTAAACGG NAG 2 0.1556 Tier II
5 NC_000017.11 17 57521946 - MSI2 NNNTCTGGCCTTGAAAAGGG NGG 2 0.0825 Tier II
6 NC_000002.12 2 157211463 + CDK7P1 NNNTCTGGCCTTGTAAACCG NGG 1 0.4483 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 36958393 - Pknox2 NNNTCTGACCTTGTAAACAG NGG 2 0.7143 Tier II
2 NC_000086.7 X 83849359 + Dmd NNNTCTGTCCTTGTAAACTG NGG 2 0.4167 Tier II
3 NC_000068.7 2 102081640 + Ldlrad3 NNNTGTGGCCTTGTAAAAGG NGG 2 0.3231 Tier II
4 NC_000081.6 15 85781856 + Ppara NNNTCTTGCCTTGTAAAAGG NGG 2 0.3077 Tier II
5 NC_000068.7 2 5372990 + Camk1d NNNTCTGGCTTTGTAAACGG NGC 2 0.0209 Tier II
6 NC_000068.7 2 128433448 + Gm36154 NNNTCAGGCCGTGTAAACGG NGG 2 0.3467 Tier III
7 NC_000084.6 18 69172034 - Gm41768 NNNTCTGTCCTTTTAAACGG NGG 2 0.1875 Tier III
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Other clones with same target sequence:

(none)