Construct: sgRNA BRDN0001162594

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTCACACCAAAGTCAACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP4K4 (9448)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76264

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 101831721 - MAP4K4 NNNCACACCAAAGTCAACTG NGG 0 1.0 Tier I
2 NC_000011.10 11 64801184 + MAP4K2 NNNCACCCCAAAGTCAGCTG NGG 2 0.0772 Tier I
3 NC_000003.12 3 100950947 + ABI3BP NNNCACACTAAAGTCAACTG NGG 1 0.875 Tier II
4 NC_000012.12 12 127080580 + LOC107984449 NNNCACATCAGAGTCAACTG NGG 2 0.5688 Tier II
5 NC_000003.12 3 173460851 - NLGN1 NNNGACATCAAAGTCAACTG NGG 2 0.4375 Tier II
6 NC_000003.12 3 194646303 + LSG1 NNNCACAACAAAGACAACTG NGG 2 0.4024 Tier II
7 NC_000013.11 13 32178023 + FRY NNNCACACCAAGGTCAAATG NGG 2 0.3889 Tier II
8 NC_000011.10 11 19809406 + NAV2 NNNCACACCATAGTCAACTA NGG 2 0.2885 Tier II
9 NC_000016.10 16 66801217 + TERB1 NNNCACATCAAAGTCACCTG NGG 2 0.1544 Tier II
10 NC_000023.11 X 17518750 + NHS NNNCACACCAGAGTCACCTG NGG 2 0.1147 Tier II
11 NC_000021.9 21 37660085 - KCNJ6 NNNCACACCAAAGCCACCTG NGG 2 0.0504 Tier II
12 NC_000004.12 4 176691571 - VEGFC NNNCACACCAAAGTCAAGTG NAG 2 0.0346 Tier II
13 NC_000021.9 21 14536938 + SAMSN1 NNNAACACCAAAGTCAACTG NGC 2 0.0187 Tier II
14 NC_000008.11 8 141245612 + SLC45A4 NNNCACACCAAAGTCACCTG NTG 2 0.0069 Tier II
15 NC_000012.12 12 19137723 - PLEKHA5 NNNCACAACAAAGTCTACTG NGG 2 0.0 Tier II
16 NC_000013.11 13 21170860 + SKA3 NNNCACACCAAAGTCAACTG NAT 2 0.0 Tier II
17 NC_000009.12 9 98830207 - GALNT12 NNNCACACCAAAGTCCCCTG NGG 2 0.0 Tier II
18 NC_000023.11 X 19797934 + SH3KBP1 NNNCACACGAAAGGCAACTG NGG 2 0.0 Tier II
19 NC_000003.12 3 194646303 + TMEM44-AS2 NNNCACAACAAAGACAACTG NGG 2 0.4024 Tier III
20 NC_000003.12 3 117750541 + LOC101926953 NNNCACCACAAAGTCAACTG NGG 2 0.2844 Tier III
21 NC_000002.12 2 219922897 + LOC105373890 NNNCACACCAAAATCACCTG NGG 2 0.1629 Tier III
22 NC_000001.11 1 234713349 + LOC124904556 NNNCACACTAAAGTCATCTG NGG 2 0.1167 Tier III
23 NC_000021.9 21 37660085 - KCNJ6-AS1 NNNCACACCAAAGCCACCTG NGG 2 0.0504 Tier III
24 NC_000014.9 14 44035772 + LINC02307 NNNCACACCAAAGTCAACTG NTG 1 0.039 Tier III
25 NC_000004.12 4 176691571 - HAFML NNNCACACCAAAGTCAAGTG NAG 2 0.0346 Tier III
26 NC_000021.9 21 14536938 + LOC124905053 NNNAACACCAAAGTCAACTG NGC 2 0.0187 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 88973649 - Olfr1213 NNNCACACCAAAGGTAACTG NGG 2 0.0 Tier I
2 NC_000078.6 12 102344010 + Rin3 NNNCACACAAAGGTCAACTG NGG 2 0.619 Tier II
3 NC_000079.6 13 45741747 - Atxn1 NNNCACAGCAAAGACAACTG NGG 2 0.398 Tier II
4 NC_000083.6 17 13749330 + Tcte2 NNNCACATCAAAGTCAACTC NGG 2 0.375 Tier II
5 NC_000069.6 3 66080947 - Veph1 NNNGACACCAAAGACAACTG NGG 2 0.3095 Tier II
6 NC_000085.6 19 28647054 + Glis3 NNNCAGACCAAAGTCAAATG NGG 2 0.2692 Tier II
7 NC_000067.6 1 17616092 - Pi15 NNNAACACCATAGTCAACTG NGG 2 0.2591 Tier II
8 NC_000079.6 13 112316147 - Ankrd55 NNNCACACCAGATTCAACTG NGG 2 0.195 Tier II
9 NC_000085.6 19 6354853 + Map4k2 NNNCAGACCCAAGTCAACTG NGG 2 0.1667 Tier II
10 NC_000083.6 17 49706591 - Kif6 NNNCACACCAAAGTAAATTG NGG 2 0.1429 Tier II
11 NC_000067.6 1 153140819 + Lamc2 NNNCACACCAGAGTCATCTG NGG 2 0.0867 Tier II
12 NC_000069.6 3 86514252 - Lrba NNNCACACCATAGGCAACTG NGG 2 0.0 Tier II
13 NC_000073.6 7 91118061 - Dlg2 NNNCACGCCAAAGTCTACTG NGG 2 0.0 Tier II
14 NC_000078.6 12 46003532 - Gm33811 NNNGACACCAAAATCAACTG NGG 2 0.4615 Tier III
15 NC_000067.6 1 88984185 - Gm34194 NNNCAGGCCAAAGTCAACTG NGG 2 0.3529 Tier III
16 NC_000067.6 1 17616092 - Gm28154 NNNAACACCATAGTCAACTG NGG 2 0.2591 Tier III
17 NC_000069.6 3 149082285 - Gm30575 NNNCACACCAAAGCCAACTG NGC 2 0.0063 Tier III
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Other clones with same target sequence:

(none)