Construct: sgRNA BRDN0001162594
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTCACACCAAAGTCAACTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- MAP4K4 (9448)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000002.12 | 2 | 101831721 | - | MAP4K4 | NNNCACACCAAAGTCAACTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000011.10 | 11 | 64801184 | + | MAP4K2 | NNNCACCCCAAAGTCAGCTG | NGG | 2 | 0.0772 | Tier I |
3 | NC_000003.12 | 3 | 100950947 | + | ABI3BP | NNNCACACTAAAGTCAACTG | NGG | 1 | 0.875 | Tier II |
4 | NC_000012.12 | 12 | 127080580 | + | LOC107984449 | NNNCACATCAGAGTCAACTG | NGG | 2 | 0.5688 | Tier II |
5 | NC_000003.12 | 3 | 173460851 | - | NLGN1 | NNNGACATCAAAGTCAACTG | NGG | 2 | 0.4375 | Tier II |
6 | NC_000003.12 | 3 | 194646303 | + | LSG1 | NNNCACAACAAAGACAACTG | NGG | 2 | 0.4024 | Tier II |
7 | NC_000013.11 | 13 | 32178023 | + | FRY | NNNCACACCAAGGTCAAATG | NGG | 2 | 0.3889 | Tier II |
8 | NC_000011.10 | 11 | 19809406 | + | NAV2 | NNNCACACCATAGTCAACTA | NGG | 2 | 0.2885 | Tier II |
9 | NC_000016.10 | 16 | 66801217 | + | TERB1 | NNNCACATCAAAGTCACCTG | NGG | 2 | 0.1544 | Tier II |
10 | NC_000023.11 | X | 17518750 | + | NHS | NNNCACACCAGAGTCACCTG | NGG | 2 | 0.1147 | Tier II |
11 | NC_000021.9 | 21 | 37660085 | - | KCNJ6 | NNNCACACCAAAGCCACCTG | NGG | 2 | 0.0504 | Tier II |
12 | NC_000004.12 | 4 | 176691571 | - | VEGFC | NNNCACACCAAAGTCAAGTG | NAG | 2 | 0.0346 | Tier II |
13 | NC_000021.9 | 21 | 14536938 | + | SAMSN1 | NNNAACACCAAAGTCAACTG | NGC | 2 | 0.0187 | Tier II |
14 | NC_000008.11 | 8 | 141245612 | + | SLC45A4 | NNNCACACCAAAGTCACCTG | NTG | 2 | 0.0069 | Tier II |
15 | NC_000012.12 | 12 | 19137723 | - | PLEKHA5 | NNNCACAACAAAGTCTACTG | NGG | 2 | 0.0 | Tier II |
16 | NC_000013.11 | 13 | 21170860 | + | SKA3 | NNNCACACCAAAGTCAACTG | NAT | 2 | 0.0 | Tier II |
17 | NC_000009.12 | 9 | 98830207 | - | GALNT12 | NNNCACACCAAAGTCCCCTG | NGG | 2 | 0.0 | Tier II |
18 | NC_000023.11 | X | 19797934 | + | SH3KBP1 | NNNCACACGAAAGGCAACTG | NGG | 2 | 0.0 | Tier II |
19 | NC_000003.12 | 3 | 194646303 | + | TMEM44-AS2 | NNNCACAACAAAGACAACTG | NGG | 2 | 0.4024 | Tier III |
20 | NC_000003.12 | 3 | 117750541 | + | LOC101926953 | NNNCACCACAAAGTCAACTG | NGG | 2 | 0.2844 | Tier III |
21 | NC_000002.12 | 2 | 219922897 | + | LOC105373890 | NNNCACACCAAAATCACCTG | NGG | 2 | 0.1629 | Tier III |
22 | NC_000001.11 | 1 | 234713349 | + | LOC124904556 | NNNCACACTAAAGTCATCTG | NGG | 2 | 0.1167 | Tier III |
23 | NC_000021.9 | 21 | 37660085 | - | KCNJ6-AS1 | NNNCACACCAAAGCCACCTG | NGG | 2 | 0.0504 | Tier III |
24 | NC_000014.9 | 14 | 44035772 | + | LINC02307 | NNNCACACCAAAGTCAACTG | NTG | 1 | 0.039 | Tier III |
25 | NC_000004.12 | 4 | 176691571 | - | HAFML | NNNCACACCAAAGTCAAGTG | NAG | 2 | 0.0346 | Tier III |
26 | NC_000021.9 | 21 | 14536938 | + | LOC124905053 | NNNAACACCAAAGTCAACTG | NGC | 2 | 0.0187 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000068.7 | 2 | 88973649 | - | Olfr1213 | NNNCACACCAAAGGTAACTG | NGG | 2 | 0.0 | Tier I |
2 | NC_000078.6 | 12 | 102344010 | + | Rin3 | NNNCACACAAAGGTCAACTG | NGG | 2 | 0.619 | Tier II |
3 | NC_000079.6 | 13 | 45741747 | - | Atxn1 | NNNCACAGCAAAGACAACTG | NGG | 2 | 0.398 | Tier II |
4 | NC_000083.6 | 17 | 13749330 | + | Tcte2 | NNNCACATCAAAGTCAACTC | NGG | 2 | 0.375 | Tier II |
5 | NC_000069.6 | 3 | 66080947 | - | Veph1 | NNNGACACCAAAGACAACTG | NGG | 2 | 0.3095 | Tier II |
6 | NC_000085.6 | 19 | 28647054 | + | Glis3 | NNNCAGACCAAAGTCAAATG | NGG | 2 | 0.2692 | Tier II |
7 | NC_000067.6 | 1 | 17616092 | - | Pi15 | NNNAACACCATAGTCAACTG | NGG | 2 | 0.2591 | Tier II |
8 | NC_000079.6 | 13 | 112316147 | - | Ankrd55 | NNNCACACCAGATTCAACTG | NGG | 2 | 0.195 | Tier II |
9 | NC_000085.6 | 19 | 6354853 | + | Map4k2 | NNNCAGACCCAAGTCAACTG | NGG | 2 | 0.1667 | Tier II |
10 | NC_000083.6 | 17 | 49706591 | - | Kif6 | NNNCACACCAAAGTAAATTG | NGG | 2 | 0.1429 | Tier II |
11 | NC_000067.6 | 1 | 153140819 | + | Lamc2 | NNNCACACCAGAGTCATCTG | NGG | 2 | 0.0867 | Tier II |
12 | NC_000069.6 | 3 | 86514252 | - | Lrba | NNNCACACCATAGGCAACTG | NGG | 2 | 0.0 | Tier II |
13 | NC_000073.6 | 7 | 91118061 | - | Dlg2 | NNNCACGCCAAAGTCTACTG | NGG | 2 | 0.0 | Tier II |
14 | NC_000078.6 | 12 | 46003532 | - | Gm33811 | NNNGACACCAAAATCAACTG | NGG | 2 | 0.4615 | Tier III |
15 | NC_000067.6 | 1 | 88984185 | - | Gm34194 | NNNCAGGCCAAAGTCAACTG | NGG | 2 | 0.3529 | Tier III |
16 | NC_000067.6 | 1 | 17616092 | - | Gm28154 | NNNAACACCATAGTCAACTG | NGG | 2 | 0.2591 | Tier III |
17 | NC_000069.6 | 3 | 149082285 | - | Gm30575 | NNNCACACCAAAGCCAACTG | NGC | 2 | 0.0063 | Tier III |