GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001162594

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: ACTCACACCAAAGTCAACTG

Original Target:

Taxon:: Homo sapiens (human)
Gene:: MAP4K4 (9448)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 76264

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000002.12	2	101831721	-	MAP4K4	NNNCACACCAAAGTCAACTG	NGG	0	1.0	Tier I
2	NC_000011.10	11	64801184	+	MAP4K2	NNNCACCCCAAAGTCAGCTG	NGG	2	0.0772	Tier I
3	NC_000003.12	3	100950947	+	ABI3BP	NNNCACACTAAAGTCAACTG	NGG	1	0.875	Tier II
4	NC_000012.12	12	127080580	+	LOC107984449	NNNCACATCAGAGTCAACTG	NGG	2	0.5688	Tier II
5	NC_000003.12	3	173460851	-	NLGN1	NNNGACATCAAAGTCAACTG	NGG	2	0.4375	Tier II
6	NC_000003.12	3	194646303	+	LSG1	NNNCACAACAAAGACAACTG	NGG	2	0.4024	Tier II
7	NC_000013.11	13	32178023	+	FRY	NNNCACACCAAGGTCAAATG	NGG	2	0.3889	Tier II
8	NC_000011.10	11	19809406	+	NAV2	NNNCACACCATAGTCAACTA	NGG	2	0.2885	Tier II
9	NC_000016.10	16	66801217	+	TERB1	NNNCACATCAAAGTCACCTG	NGG	2	0.1544	Tier II
10	NC_000023.11	X	17518750	+	NHS	NNNCACACCAGAGTCACCTG	NGG	2	0.1147	Tier II
11	NC_000021.9	21	37660085	-	KCNJ6	NNNCACACCAAAGCCACCTG	NGG	2	0.0504	Tier II
12	NC_000004.12	4	176691571	-	VEGFC	NNNCACACCAAAGTCAAGTG	NAG	2	0.0346	Tier II
13	NC_000021.9	21	14536938	+	SAMSN1	NNNAACACCAAAGTCAACTG	NGC	2	0.0187	Tier II
14	NC_000008.11	8	141245612	+	SLC45A4	NNNCACACCAAAGTCACCTG	NTG	2	0.0069	Tier II
15	NC_000012.12	12	19137723	-	PLEKHA5	NNNCACAACAAAGTCTACTG	NGG	2	0.0	Tier II
16	NC_000013.11	13	21170860	+	SKA3	NNNCACACCAAAGTCAACTG	NAT	2	0.0	Tier II
17	NC_000009.12	9	98830207	-	GALNT12	NNNCACACCAAAGTCCCCTG	NGG	2	0.0	Tier II
18	NC_000023.11	X	19797934	+	SH3KBP1	NNNCACACGAAAGGCAACTG	NGG	2	0.0	Tier II
19	NC_000003.12	3	194646303	+	TMEM44-AS2	NNNCACAACAAAGACAACTG	NGG	2	0.4024	Tier III
20	NC_000003.12	3	117750541	+	LOC101926953	NNNCACCACAAAGTCAACTG	NGG	2	0.2844	Tier III
21	NC_000002.12	2	219922897	+	LOC105373890	NNNCACACCAAAATCACCTG	NGG	2	0.1629	Tier III
22	NC_000001.11	1	234713349	+	LOC124904556	NNNCACACTAAAGTCATCTG	NGG	2	0.1167	Tier III
23	NC_000021.9	21	37660085	-	KCNJ6-AS1	NNNCACACCAAAGCCACCTG	NGG	2	0.0504	Tier III
24	NC_000014.9	14	44035772	+	LINC02307	NNNCACACCAAAGTCAACTG	NTG	1	0.039	Tier III
25	NC_000004.12	4	176691571	-	HAFML	NNNCACACCAAAGTCAAGTG	NAG	2	0.0346	Tier III
26	NC_000021.9	21	14536938	+	LOC124905053	NNNAACACCAAAGTCAACTG	NGC	2	0.0187	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000068.7	2	88973649	-	Olfr1213	NNNCACACCAAAGGTAACTG	NGG	2	0.0	Tier I
2	NC_000078.6	12	102344010	+	Rin3	NNNCACACAAAGGTCAACTG	NGG	2	0.619	Tier II
3	NC_000079.6	13	45741747	-	Atxn1	NNNCACAGCAAAGACAACTG	NGG	2	0.398	Tier II
4	NC_000083.6	17	13749330	+	Tcte2	NNNCACATCAAAGTCAACTC	NGG	2	0.375	Tier II
5	NC_000069.6	3	66080947	-	Veph1	NNNGACACCAAAGACAACTG	NGG	2	0.3095	Tier II
6	NC_000085.6	19	28647054	+	Glis3	NNNCAGACCAAAGTCAAATG	NGG	2	0.2692	Tier II
7	NC_000067.6	1	17616092	-	Pi15	NNNAACACCATAGTCAACTG	NGG	2	0.2591	Tier II
8	NC_000079.6	13	112316147	-	Ankrd55	NNNCACACCAGATTCAACTG	NGG	2	0.195	Tier II
9	NC_000085.6	19	6354853	+	Map4k2	NNNCAGACCCAAGTCAACTG	NGG	2	0.1667	Tier II
10	NC_000083.6	17	49706591	-	Kif6	NNNCACACCAAAGTAAATTG	NGG	2	0.1429	Tier II
11	NC_000067.6	1	153140819	+	Lamc2	NNNCACACCAGAGTCATCTG	NGG	2	0.0867	Tier II
12	NC_000069.6	3	86514252	-	Lrba	NNNCACACCATAGGCAACTG	NGG	2	0.0	Tier II
13	NC_000073.6	7	91118061	-	Dlg2	NNNCACGCCAAAGTCTACTG	NGG	2	0.0	Tier II
14	NC_000078.6	12	46003532	-	Gm33811	NNNGACACCAAAATCAACTG	NGG	2	0.4615	Tier III
15	NC_000067.6	1	88984185	-	Gm34194	NNNCAGGCCAAAGTCAACTG	NGG	2	0.3529	Tier III
16	NC_000067.6	1	17616092	-	Gm28154	NNNAACACCATAGTCAACTG	NGG	2	0.2591	Tier III
17	NC_000069.6	3	149082285	-	Gm30575	NNNCACACCAAAGCCAACTG	NGC	2	0.0063	Tier III

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Other clones with same target sequence:

(none)