Construct: sgRNA BRDN0001162598
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGTCAAGCGGGCCACCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NUAK1 (9891)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75577
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
106138435 |
- |
NUAK1 |
NNNTCAAGCGGGCCACCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
76043481 |
+ |
STYXL1 |
NNNCCAAGAGGGCCACCGAG |
NGG |
2 |
0.5546 |
Tier II |
| 3 |
NC_000002.12 |
2 |
238860125 |
- |
TWIST2 |
NNNTGAAGCGGTCCACCGAG |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000022.11 |
22 |
46776085 |
+ |
TBC1D22A |
NNNCCAAGCGGGCCACGGAG |
NGG |
2 |
0.0381 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
84440136 |
- |
Nuak1 |
NNNTCAAGAGAGCCACCGAG |
NGG |
2 |
0.8571 |
Tier I |
| 2 |
NC_000077.6 |
11 |
97666939 |
+ |
Mllt6 |
NNNTCAAGGTGGCCACCGAG |
NGG |
2 |
0.503 |
Tier II |
| 3 |
NC_000073.6 |
7 |
82335501 |
- |
Adamtsl3 |
NNNACAAGCGGGCCACCGAG |
NCG |
2 |
0.051 |
Tier II |
| 4 |
NC_000079.6 |
13 |
9017404 |
- |
Gm36264 |
NNNTCAAGCGGGCCACTGAG |
NGC |
2 |
0.0104 |
Tier III |
Other clones with same target sequence:
(none)
