Construct: sgRNA BRDN0001487044
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGCTGGAACCGTAAGGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EXOSC10 (5394)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77988
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
11091493 |
- |
EXOSC10 |
NNNGCTGGAACCGTAAGGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
124815046 |
+ |
SCARB1 |
NNNGCTGGAACTGGAAGGTG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000008.11 |
8 |
1356312 |
- |
DLGAP2 |
NNNGTTGAAACCGTAAGGTG |
NGG |
2 |
0.6364 |
Tier II |
| 4 |
NC_000016.10 |
16 |
5028379 |
+ |
NAGPA |
NNNGCTGTAACCGTAAGGTG |
NGC |
2 |
0.0139 |
Tier II |
| 5 |
NC_000002.12 |
2 |
45611270 |
- |
SRBD1 |
NNNGCGGGAACCGGAAGGTG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000020.11 |
20 |
63776999 |
- |
ZBTB46 |
NNNGCTGGAACCGTATTGTG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000008.11 |
8 |
1356312 |
- |
LOC124901869 |
NNNGTTGAAACCGTAAGGTG |
NGG |
2 |
0.6364 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
67752369 |
- |
Lama1 |
NNNGCTGGAAGAGTAAGGTG |
NGG |
2 |
0.1786 |
Tier II |
| 2 |
NC_000081.6 |
15 |
27895092 |
+ |
Trio |
NNNGCTGGAACCCTAACGTG |
NGG |
2 |
0.0991 |
Tier II |
| 3 |
NC_000080.6 |
14 |
25846451 |
+ |
Anxa11 |
NNNGCTGGAACCTTAAGGTG |
NAG |
2 |
0.0778 |
Tier II |
| 4 |
NC_000068.7 |
2 |
31688434 |
+ |
Abl1 |
NNNGCTGGAACCCGAAGGTG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000083.6 |
17 |
5201723 |
- |
Arid1b |
NNNGCTGGAACCGGAAGGTG |
NGC |
2 |
0.0 |
Tier II |
| 6 |
NC_000068.7 |
2 |
31688434 |
+ |
Gm13427 |
NNNGCTGGAACCCGAAGGTG |
NGG |
2 |
0.0 |
Tier III |
| 7 |
NC_000083.6 |
17 |
5201723 |
- |
Gm34854 |
NNNGCTGGAACCGGAAGGTG |
NGC |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
