Construct: sgRNA BRDN0001487045

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATATCCAACATAAAAGCTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GK2 (2712)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77911

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 79407941 - GK2 NNNTCCAACATAAAAGCTGT NGG 0 1.0 Tier I
2 NC_000004.12 4 165279386 - GK3 NNNTCCAACATAAAAGCTAT NGG 1 0.7143 Tier I
3 NC_000001.11 1 65491095 + LEPR NNNTCCAAAATAAAAACTGT NGG 2 0.8571 Tier II
4 NC_000011.10 11 16047907 - SOX6 NNNTCCAAAATAAAAGCTGT NGG 1 0.8571 Tier II
5 NC_000014.9 14 50269750 - L2HGDH NNNTCCAAATTAAAAGCTGT NGG 2 0.7563 Tier II
6 NC_000004.12 4 165279386 - KLHL2 NNNTCCAACATAAAAGCTAT NGG 1 0.7143 Tier II
7 NC_000019.10 19 57162505 - DUXA NNNTCAAACAAAAAAGCTGT NGG 2 0.6964 Tier II
8 NC_000011.10 11 119303690 - CBL NNNTCCAACATAAAAACAGT NGG 2 0.6667 Tier II
9 NC_000006.12 6 125222617 + TPD52L1 NNNTTCAAAATAAAAGCTGT NGG 2 0.5455 Tier II
10 NC_000009.12 9 99968121 + STX17 NNNTGCAACATAAAAGCTTT NGG 2 0.4 Tier II
11 NC_000009.12 9 32517356 + RIGI NNNTCCAAGGTAAAAGCTGT NGG 2 0.3439 Tier II
12 NC_000022.11 22 24027533 - CABIN1 NNNTCCAACATATAAACTGT NGG 2 0.3 Tier II
13 NC_000002.12 2 241196153 + ANO7 NNNTCCAACATTAAAGCTTT NGG 2 0.2222 Tier II
14 NC_000022.11 22 20530194 + MED15 NNNTCCCACATACAAGCTGT NGG 2 0.0921 Tier II
15 NC_000012.12 12 65945213 + HMGA2 NNNTCTAACATAAAAGGTGT NGG 2 0.0546 Tier II
16 NC_000007.14 7 148759822 + CUL1 NNNTCCATCATAAAAGCTGT NTG 2 0.0167 Tier II
17 NC_000010.11 10 107043346 - SORCS1 NNNTCCAACATAAAACCTGC NGG 2 0.0 Tier II
18 NC_000003.12 3 114849034 + ZBTB20 NNNTCCAACATAAAACCTGT NGA 2 0.0 Tier II
19 NC_000001.11 1 60601348 + LINC01748 NNNTCAAAAATAAAAGCTGT NGG 2 0.7959 Tier III
20 NC_000004.12 4 119394698 - GK6P NNNTCCAACATAAAAGCTAT NGG 1 0.7143 Tier III
21 NC_000004.12 4 119394698 - LOC107986193 NNNTCCAACATAAAAGCTAT NGG 1 0.7143 Tier III
22 NC_000023.11 X 101780281 - GK4P NNNTCCAACATAAAAGCTAT NGG 1 0.7143 Tier III
23 NC_000004.12 4 189066514 - LOC105377612 NNNTCTAACATGAAAGCTGT NGG 2 0.6706 Tier III
24 NC_000011.10 11 57264260 + LOC107984364 NNNTCCAAAATAAAAGCTAT NGG 2 0.6122 Tier III
25 NC_000005.10 5 1724574 + LOC105374614 NNNTCCAACATGAAAGCCGT NGG 2 0.3095 Tier III
26 NC_000009.12 9 85415287 + LOC105376121 NNNAGCAACATAAAAGCTGT NGG 2 0.2857 Tier III
27 NC_000015.10 15 51047099 - MIR4713HG NNNTCCAACATAAAAACTGC NGG 2 0.0909 Tier III
28 NC_000012.12 12 65945213 + LOC124902955 NNNTCTAACATAAAAGGTGT NGG 2 0.0546 Tier III
29 NC_000004.12 4 111331406 - RNU6-289P NNNTCCAACATAAAATCTGT NGT 2 0.0 Tier III
30 NC_000003.12 3 177749345 - LINC00578 NNNTCCAACATAAAATCTTT NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 52693982 + Gykl1 NNNTCCAACATAAAAGCTAT NGG 1 0.7143 Tier I
2 NC_000071.6 5 97456718 - Gk2 NNNTCCAACATTAAAGCTAT NGG 2 0.2381 Tier I
3 NC_000068.7 2 121025824 - Epb42 NNNTCCAACAGAAAAGATGT NGG 2 0.1867 Tier I
4 NC_000070.6 4 48391014 - Invs NNNTCTGACATAAAAGCTGT NGG 2 0.6555 Tier II
5 NC_000068.7 2 172373605 - Cstf1 NNNTCAAACATAAAGGCTGT NGG 2 0.6036 Tier II
6 NC_000072.6 6 21626041 + Kcnd2 NNNTCCAACTCAAAAGCTGT NGG 2 0.5882 Tier II
7 NC_000073.6 7 57379622 + Gabrg3 NNNTCCAATACAAAAGCTGT NGG 2 0.5833 Tier II
8 NC_000071.6 5 137153641 - Muc3 NNNTCAAACATAAAAGCTGA NGG 2 0.5223 Tier II
9 NC_000082.6 16 66854418 - Cadm2 NNNTCCAACATGGAAGCTGT NGG 2 0.471 Tier II
10 NC_000072.6 6 46703501 - Cntnap2 NNNTTCAACATAAAAGCTTT NGG 2 0.4242 Tier II
11 NC_000082.6 16 17682878 - Med15 NNNTCACACATAAAAGCTGT NGG 2 0.4062 Tier II
12 NC_000081.6 15 20994910 + Cdh12 NNNACCAACATGAAAGCTGT NGG 2 0.3439 Tier II
13 NC_000075.6 9 10529401 + Cntn5 NNNTCCAGCATAAGAGCTGT NGG 2 0.3422 Tier II
14 NC_000076.6 10 70252514 - Slc16a9 NNNTCCAGCATAAGAGCTGT NGG 2 0.3422 Tier II
15 NC_000076.6 10 116470185 - Kcnmb4 NNNTTCAACATAAAAGATGT NGG 2 0.297 Tier II
16 NC_000069.6 3 18231846 - Cyp7b1 NNNACCTACATAAAAGCTGT NGG 2 0.2083 Tier II
17 NC_000083.6 17 11376221 - Prkn NNNTCCAAGATCAAAGCTGT NGG 2 0.1629 Tier II
18 NC_000073.6 7 91335248 - Dlg2 NNNTCCAACATAAAAGCTCT NAG 2 0.1162 Tier II
19 NC_000079.6 13 59461396 + Agtpbp1 NNNTCCACCATCAAAGCTGT NGG 2 0.1128 Tier II
20 NC_000072.6 6 18257220 - Cftr NNNTCCAACAGCAAAGCTGT NGG 2 0.1053 Tier II
21 NC_000079.6 13 67745940 - Zfp85 NNNTCCAACATATATGCTGT NGG 2 0.06 Tier II
22 NC_000076.6 10 34091724 + Calhm5 NNNTCCAACTTAAAAGGTGT NGG 2 0.0519 Tier II
23 NC_000076.6 10 34091724 + Trappc3l NNNTCCAACTTAAAAGGTGT NGG 2 0.0519 Tier II
24 NC_000080.6 14 76440850 - Tsc22d1 NNNTCCAACTTAAAAGGTGT NGG 2 0.0519 Tier II
25 NC_000085.6 19 37108336 + Cpeb3 NNNTCCAACATGAAAGCTGT NGA 2 0.0502 Tier II
26 NC_000074.6 8 108485719 - Zfhx3 NNNTCCAACATCAAAGCTGT NGA 2 0.0183 Tier II
27 NC_000076.6 10 28492189 + Ptprk NNNTACAACATAAAAGCTGT NGT 2 0.0092 Tier II
28 NC_000078.6 12 85963559 - Ttll5 NNNTCCAACATAAAAGCTGT NTT 2 0.0 Tier II
29 NC_000072.6 6 88621217 - Kbtbd12 NNNTCCAACATAAAATCTGT NAG 2 0.0 Tier II
30 NC_000080.6 14 68523400 - Adam7 NNNTTCAACATAAAATCTGT NGG 2 0.0 Tier II
31 NC_000071.6 5 97456718 - 4930467D21Rik NNNTCCAACATTAAAGCTAT NGG 2 0.2381 Tier III
32 NC_000076.6 10 19160771 - Gm20139 NNNTCCAACATTGAAGCTGT NGG 2 0.2174 Tier III
33 NC_000079.6 13 33514018 + Gm11387 NNNTCCACCATAAAAGTTGT NGG 2 0.2 Tier III
34 NC_000069.6 3 69808906 + Gm19055 NNNTCCAACATATAGGCTGT NGG 2 0.195 Tier III
35 NC_000072.6 6 145517386 - Gm38475 NNNTCCAACATCAAAGCCGT NGG 2 0.1128 Tier III
36 NC_000079.6 13 67745940 - Zfp85os NNNTCCAACATATATGCTGT NGG 2 0.06 Tier III
Download CSV

Other clones with same target sequence:

(none)