Construct: sgRNA BRDN0001487045
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATATCCAACATAAAAGCTGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GK2 (2712)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77911
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000084.6 |
18 |
52693982 |
+ |
Gykl1 |
NNNTCCAACATAAAAGCTAT |
NGG |
1 |
0.7143 |
Tier I |
2 |
NC_000071.6 |
5 |
97456718 |
- |
Gk2 |
NNNTCCAACATTAAAGCTAT |
NGG |
2 |
0.2381 |
Tier I |
3 |
NC_000068.7 |
2 |
121025824 |
- |
Epb42 |
NNNTCCAACAGAAAAGATGT |
NGG |
2 |
0.1867 |
Tier I |
4 |
NC_000070.6 |
4 |
48391014 |
- |
Invs |
NNNTCTGACATAAAAGCTGT |
NGG |
2 |
0.6555 |
Tier II |
5 |
NC_000068.7 |
2 |
172373605 |
- |
Cstf1 |
NNNTCAAACATAAAGGCTGT |
NGG |
2 |
0.6036 |
Tier II |
6 |
NC_000072.6 |
6 |
21626041 |
+ |
Kcnd2 |
NNNTCCAACTCAAAAGCTGT |
NGG |
2 |
0.5882 |
Tier II |
7 |
NC_000073.6 |
7 |
57379622 |
+ |
Gabrg3 |
NNNTCCAATACAAAAGCTGT |
NGG |
2 |
0.5833 |
Tier II |
8 |
NC_000071.6 |
5 |
137153641 |
- |
Muc3 |
NNNTCAAACATAAAAGCTGA |
NGG |
2 |
0.5223 |
Tier II |
9 |
NC_000082.6 |
16 |
66854418 |
- |
Cadm2 |
NNNTCCAACATGGAAGCTGT |
NGG |
2 |
0.471 |
Tier II |
10 |
NC_000072.6 |
6 |
46703501 |
- |
Cntnap2 |
NNNTTCAACATAAAAGCTTT |
NGG |
2 |
0.4242 |
Tier II |
11 |
NC_000082.6 |
16 |
17682878 |
- |
Med15 |
NNNTCACACATAAAAGCTGT |
NGG |
2 |
0.4062 |
Tier II |
12 |
NC_000081.6 |
15 |
20994910 |
+ |
Cdh12 |
NNNACCAACATGAAAGCTGT |
NGG |
2 |
0.3439 |
Tier II |
13 |
NC_000075.6 |
9 |
10529401 |
+ |
Cntn5 |
NNNTCCAGCATAAGAGCTGT |
NGG |
2 |
0.3422 |
Tier II |
14 |
NC_000076.6 |
10 |
70252514 |
- |
Slc16a9 |
NNNTCCAGCATAAGAGCTGT |
NGG |
2 |
0.3422 |
Tier II |
15 |
NC_000076.6 |
10 |
116470185 |
- |
Kcnmb4 |
NNNTTCAACATAAAAGATGT |
NGG |
2 |
0.297 |
Tier II |
16 |
NC_000069.6 |
3 |
18231846 |
- |
Cyp7b1 |
NNNACCTACATAAAAGCTGT |
NGG |
2 |
0.2083 |
Tier II |
17 |
NC_000083.6 |
17 |
11376221 |
- |
Prkn |
NNNTCCAAGATCAAAGCTGT |
NGG |
2 |
0.1629 |
Tier II |
18 |
NC_000073.6 |
7 |
91335248 |
- |
Dlg2 |
NNNTCCAACATAAAAGCTCT |
NAG |
2 |
0.1162 |
Tier II |
19 |
NC_000079.6 |
13 |
59461396 |
+ |
Agtpbp1 |
NNNTCCACCATCAAAGCTGT |
NGG |
2 |
0.1128 |
Tier II |
20 |
NC_000072.6 |
6 |
18257220 |
- |
Cftr |
NNNTCCAACAGCAAAGCTGT |
NGG |
2 |
0.1053 |
Tier II |
21 |
NC_000079.6 |
13 |
67745940 |
- |
Zfp85 |
NNNTCCAACATATATGCTGT |
NGG |
2 |
0.06 |
Tier II |
22 |
NC_000076.6 |
10 |
34091724 |
+ |
Calhm5 |
NNNTCCAACTTAAAAGGTGT |
NGG |
2 |
0.0519 |
Tier II |
23 |
NC_000076.6 |
10 |
34091724 |
+ |
Trappc3l |
NNNTCCAACTTAAAAGGTGT |
NGG |
2 |
0.0519 |
Tier II |
24 |
NC_000080.6 |
14 |
76440850 |
- |
Tsc22d1 |
NNNTCCAACTTAAAAGGTGT |
NGG |
2 |
0.0519 |
Tier II |
25 |
NC_000085.6 |
19 |
37108336 |
+ |
Cpeb3 |
NNNTCCAACATGAAAGCTGT |
NGA |
2 |
0.0502 |
Tier II |
26 |
NC_000074.6 |
8 |
108485719 |
- |
Zfhx3 |
NNNTCCAACATCAAAGCTGT |
NGA |
2 |
0.0183 |
Tier II |
27 |
NC_000076.6 |
10 |
28492189 |
+ |
Ptprk |
NNNTACAACATAAAAGCTGT |
NGT |
2 |
0.0092 |
Tier II |
28 |
NC_000078.6 |
12 |
85963559 |
- |
Ttll5 |
NNNTCCAACATAAAAGCTGT |
NTT |
2 |
0.0 |
Tier II |
29 |
NC_000072.6 |
6 |
88621217 |
- |
Kbtbd12 |
NNNTCCAACATAAAATCTGT |
NAG |
2 |
0.0 |
Tier II |
30 |
NC_000080.6 |
14 |
68523400 |
- |
Adam7 |
NNNTTCAACATAAAATCTGT |
NGG |
2 |
0.0 |
Tier II |
31 |
NC_000071.6 |
5 |
97456718 |
- |
4930467D21Rik |
NNNTCCAACATTAAAGCTAT |
NGG |
2 |
0.2381 |
Tier III |
32 |
NC_000076.6 |
10 |
19160771 |
- |
Gm20139 |
NNNTCCAACATTGAAGCTGT |
NGG |
2 |
0.2174 |
Tier III |
33 |
NC_000079.6 |
13 |
33514018 |
+ |
Gm11387 |
NNNTCCACCATAAAAGTTGT |
NGG |
2 |
0.2 |
Tier III |
34 |
NC_000069.6 |
3 |
69808906 |
+ |
Gm19055 |
NNNTCCAACATATAGGCTGT |
NGG |
2 |
0.195 |
Tier III |
35 |
NC_000072.6 |
6 |
145517386 |
- |
Gm38475 |
NNNTCCAACATCAAAGCCGT |
NGG |
2 |
0.1128 |
Tier III |
36 |
NC_000079.6 |
13 |
67745940 |
- |
Zfp85os |
NNNTCCAACATATATGCTGT |
NGG |
2 |
0.06 |
Tier III |
Other clones with same target sequence:
(none)